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Molecular mechanisms of ion transport and clinical application

离子转运的分子机制及临床应用

基本信息

批准号：
10044262
负责人：
HAYAKAWA Tetsuo
金额：
$ 2.69万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Pancreatic HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion were studied by monitoring luminal pH and luminal volume simultaneously in interlobular duct segments (diameter〜100mm) is isolated from guinea-pig pancreas. We found that HCOィイD23ィエD2ィイD1-ィエD1 uptake across the basolateral membrane is achieved largely by NaィイD1+ィエD1-HCOィイD23ィエD2ィイD1-ィエD1 cotransport, and that HCOィイD23ィエD2ィイD1-ィエD1 efflux and fluid secretion across the luminal membrane involve HCOィイD23ィエD2ィイD1-ィエD1 conductance. The location of purinoceptors in pancreatic duct cells and their role in regulating HCOィイD23ィエD2ィイD1-ィエD1 and fluid secretion was studied in microperfused duct segments. We found that both apical and basolateral nucleotides inhibit it. The efficients of arginine vasopressin (AVP) on ductal secretion were studied in isolated vascularly perfused ductal fluid secretion via VィイD21ィエD2 receptors.2. We have adopted molecular methodologies to study water transport and cloned a new aquaporin from rat liver with high … More amino-acid sequence identify to human AQP9.3. We also conduct study of water and electrolytes transport in vivo. We found that guanylin and heat-stable enterotoxin administered into the lumen of rat jejunum caused fluid secretion. Intravenous guanylin induced mucus secretion from goblet cells in rat duodenal crypts.4. We have succeeded in a continuous and simultaneous measurement of pancreatic blood flow and pancreatic secretion in conscious dog. We found that pituitary adenylate cyclase activating peptide (PACAP) and vasoactive intestinal peptide (VIP) induced vasodilatation comparable to that observed after a meal.5. We have started to investigate the relationships between mutations in cystic fibrosis transmembrane conductance regulator (CFTR) and chronic pancreatitis in Japan.6. We examined the effects of phospholipase AィイD22ィエD2 (PLAィイD22ィエD2) inhibitors on CaィイD12+ィエD1 oscillations and amylase secretion in rat pancreatic acinar cells. We found that type IV (cytosolic)PLAィイD22ィエD2 regulates CaィイD12+ィエD1 signaling and enzyme secretion. Less

1. 胰腺HCOィイc15ィエD2ィイD1 -ィエD1和液体分泌研究通过监测腔的pH值和小叶间导管腔的体积同时段(~ 100毫米)直径从豚鼠胰腺是孤立的。我们发现HCOィイc15ィエD2ィイD1 -ィエD1在基底外侧膜吸收是主要由NaィイD1 +ィエD1-HCOィイc15ィエD2ィイD1 -ィエD1协同转运,这HCOィイc15ィエD2ィイD1 -ィエD1射流和液体分泌腔的膜包括HCOィイc15ィエD2ィイD1 -ィエD1电导。purinoceptors在胰管细胞的位置和他们的角色在调节HCOィイc15ィエD2ィイD1 -ィエD1和液体分泌microperfused管部分进行了研究。我们发现顶端和底侧核苷酸都抑制它。本文研究了精氨酸抗利尿素（AVP）对离体血管灌注经V - γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ的影响。采用分子方法研究了水的转运，从大鼠肝脏中克隆了一个新的水通道蛋白，其氨基酸序列与人类AQP9.3氨基酸序列一致。我们还进行了体内水和电解质运输的研究。我们发现观音林和热稳定型肠毒素进入大鼠空肠管腔后引起液体分泌。静脉注射观音碱诱导大鼠十二指肠隐窝杯状细胞分泌粘液。我们已经成功地在有意识的狗的胰腺血流量和胰腺分泌的连续和同时测量。我们发现垂体腺苷酸环化酶激活肽（PACAP）和血管活性肠肽（VIP）诱导的血管舒张与饭后观察的血管舒张相似。在日本，我们已经开始研究囊性纤维化跨膜传导调节因子（CFTR）突变与慢性胰腺炎之间的关系。我们研究了磷脂酶A γ γ γ γ γ γ (PLA γ γ γ γ γ γ)抑制剂对大鼠胰腺腺泡细胞Ca γ γ γ γ γ γ + γ γ γ γ γ γ振荡和淀粉酶分泌的影响。我们发现IV型（细胞质）PLA D22 D2调节Ca D12+ D1信号和酶分泌。少