Various types of hemostasis for a rat esophageal varices bleeding model

1.首次采用奥林巴斯超薄硬质自视镜建立大鼠食道系统。大鼠食道内窥镜检查宜使用直径1.7 mm的硬质自体内窥镜SES1711D或SES1717D。通过使用视频内窥镜，内窥镜图像的分辨率和色彩性能明显更好。与自窥镜相连的视频图像可以被数字编码并存储在照相介质和U-马季奇录像带上。2.通过导管对大鼠胃进行持续充气，对大鼠食道进行长时间的内窥镜检查。3.体重约300g的Wistar ST大鼠部分门静脉结扎后，大鼠的食道静脉曲张发生率较高。Wistar ST大鼠20号门静脉结扎后2周建立大鼠食道静脉曲张模型是有效和经济的。4.大鼠尾静脉注射1000U肝素后，用30号EIS针刺入大鼠EIS管，造成大鼠食道静脉曲张出血模型。通过建立大鼠食道静脉曲张出血模型，对不同止血方法的止血效果进行比较。(1)腔内注射硬化疗法(EIS)是最有效的止血方法。对于硬化剂，EO和AS均有效。(2)EIS优于电凝和加温探针法。(3)对于血管反应性药物，心得安和加压素均有效，但加压素比心得安更有效。(4)EIS与加压素或心得安联合治疗对大鼠食道静脉曲张出血模型的止血效果无统计学意义。(5)凝血酶分散法优于其他方法，但止血方法简单。6.本方案可为临床治疗静脉曲张破裂出血提供参考。

1.We set up first the rat esophageal system using the Olympus thin, rigid Selfoscope. For rat esophageal endoscopy it is suitable to use the rigid Selfoscope, SES1711D or SES1717D with 1.7 mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photograficmedium and U-matic video tape.2.Long time endoscopic examination for rat esophagus was performed by continuous air feeding by piping of rat stomach through the catheter.3.The production of esophageal varices in the rat is greater in Wistar ST rats weighing about 300g with 20-gauge partial portal vein ligation. It is efficient and economical for Wistar ST rats 2-weeks afer 20-gauge portal vein ligation to be used a rat esophageal varices model.4.The rat esophageal varices bleeding model was produced by stinging from the rat EIS tube by a 30-gauge EIS needle after intra-caudalvenous injection of 1000U heparin. The estimate of the grade of rat esophageal bleeding was decided the time from start of the bleeding to hemostasis.5.Hemostasis of various types of methods was performed using this rat esophageal varices bleeding model.(1)Endoscopic injection sclerotherapy(EIS) is the most efficient hemostatic method. For sclerosing agents, EO and AS were both effective.(2)EIS was superior than electrocoagulation and heater probe method.(3)For vasoreactive drugs, propranolol and vasopressin were both effective, but vasopressin was more effevtive than propranolol, significantlly.(4)Combination therapy with EIS and vasopressin or propranolol was not statistically significant for hemostasis of rat esophageal varices bleeding model.(5)Scattering of thrombin is inferior than others but it is easy hemostatic method.6.This project was useful for clinical methods hemostasis for variceal ruputure bleeding.