Various types of hemostasis for a rat esophageal varices bleeding model

1.我们首先使用Olympus薄的刚性Selfoscope建立大鼠食管系统。对于大鼠食管内窥镜检查，适合使用直径1.7 mm的刚性Selfoscope、SES 1711 D或SES 1717 D。通过使用视频内窥镜，内窥镜图像的分辨率能力和颜色性能明显更好。2.大鼠食管内窥镜检查采用胃管持续充气法进行。3.体重约300g的Wistar ST大鼠，结扎20号门静脉后，食管静脉曲张的发生率较高。20G门静脉结扎后2周，Wistar ST大鼠作为食管静脉曲张模型是有效和经济的。4.尾静脉注射1000U肝素后，用30G EIS针刺入大鼠EIS管，建立大鼠食管静脉曲张出血模型。以出血至止血时间作为判断食管静脉曲张出血程度的指标。5.采用大鼠食管静脉曲张出血模型，进行不同止血方法的止血。（1）内镜注射硬化疗法（EIS）是最有效的止血方法。对于硬化剂，EO和AS都是有效的。（2）电化学阻抗法上级电凝法和加热探头法。（3）血管活性药物中，普萘洛尔和加压素均有效，但加压素的作用明显强于普萘洛尔。（4）EIS与加压素或心得安联合应用对大鼠食管静脉曲张出血模型的止血作用无统计学意义。（5）凝血酶的分散性较差，但止血方法简便。（6）该方案对食管静脉曲张破裂出血的临床止血方法有一定的参考价值。

1.We set up first the rat esophageal system using the Olympus thin, rigid Selfoscope. For rat esophageal endoscopy it is suitable to use the rigid Selfoscope, SES1711D or SES1717D with 1.7 mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photograficmedium and U-matic video tape.2.Long time endoscopic examination for rat esophagus was performed by continuous air feeding by piping of rat stomach through the catheter.3.The production of esophageal varices in the rat is greater in Wistar ST rats weighing about 300g with 20-gauge partial portal vein ligation. It is efficient and economical for Wistar ST rats 2-weeks afer 20-gauge portal vein ligation to be used a rat esophageal varices model.4.The rat esophageal varices bleeding model was produced by stinging from the rat EIS tube by a 30-gauge EIS needle after intra-caudalvenous injection of 1000U heparin. The estimate of the grade of rat esophageal bleeding was decided the time from start of the bleeding to hemostasis.5.Hemostasis of various types of methods was performed using this rat esophageal varices bleeding model.(1)Endoscopic injection sclerotherapy(EIS) is the most efficient hemostatic method. For sclerosing agents, EO and AS were both effective.(2)EIS was superior than electrocoagulation and heater probe method.(3)For vasoreactive drugs, propranolol and vasopressin were both effective, but vasopressin was more effevtive than propranolol, significantlly.(4)Combination therapy with EIS and vasopressin or propranolol was not statistically significant for hemostasis of rat esophageal varices bleeding model.(5)Scattering of thrombin is inferior than others but it is easy hemostatic method.6.This project was useful for clinical methods hemostasis for variceal ruputure bleeding.