Various types of hemostasis for a rat esophageal varices bleeding model

1.我们首先用奥林巴斯薄刚性自窥镜建立了大鼠食管系统。大鼠食管内窥镜宜选用刚性Selfoscope、SES1711D或直径1.7 mm的SES1717D。使用视频内窥镜，内窥镜图像的分辨率和色彩性能都有明显提高。与自窥镜相连的视屏图像可以进行数字编码，并存储在摄影介质和U-matic录像带上。采用大鼠胃管经导管连续充气的方法，对大鼠食管进行长时间内镜检查。体重约300g的Wistar ST大鼠在20径部分门静脉结扎后产生的食管静脉曲张更大。Wistar ST大鼠在20号门静脉结扎2周后用于大鼠食管静脉曲张模型是有效和经济的。采用尾静脉注射1000U肝素后，用30号EIS针穿刺大鼠食管静脉曲张出血模型。大鼠食管出血的分级以出血开始至止血的时间为判断标准。采用各种止血方法对大鼠食管静脉曲张出血模型进行止血。(1)内镜下注射硬化疗法（EIS）是最有效的止血方法。对于硬化剂，EO和AS均有效。(2)EIS法优于电凝法和热探针法。(3)对于血管反应性药物，心得安和利尿加压素均有效，但利尿加压素比心得安更有效，且效果显著。(4) EIS联合加压素或心得安对食管静脉曲张出血模型大鼠止血效果无统计学意义。(5)凝血酶散射较其他止血方法差，但简便易行。该方案对静脉曲张破裂出血的临床止血有一定的指导意义。

1.We set up first the rat esophageal system using the Olympus thin, rigid Selfoscope. For rat esophageal endoscopy it is suitable to use the rigid Selfoscope, SES1711D or SES1717D with 1.7 mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photograficmedium and U-matic video tape.2.Long time endoscopic examination for rat esophagus was performed by continuous air feeding by piping of rat stomach through the catheter.3.The production of esophageal varices in the rat is greater in Wistar ST rats weighing about 300g with 20-gauge partial portal vein ligation. It is efficient and economical for Wistar ST rats 2-weeks afer 20-gauge portal vein ligation to be used a rat esophageal varices model.4.The rat esophageal varices bleeding model was produced by stinging from the rat EIS tube by a 30-gauge EIS needle after intra-caudalvenous injection of 1000U heparin. The estimate of the grade of rat esophageal bleeding was decided the time from start of the bleeding to hemostasis.5.Hemostasis of various types of methods was performed using this rat esophageal varices bleeding model.(1)Endoscopic injection sclerotherapy(EIS) is the most efficient hemostatic method. For sclerosing agents, EO and AS were both effective.(2)EIS was superior than electrocoagulation and heater probe method.(3)For vasoreactive drugs, propranolol and vasopressin were both effective, but vasopressin was more effevtive than propranolol, significantlly.(4)Combination therapy with EIS and vasopressin or propranolol was not statistically significant for hemostasis of rat esophageal varices bleeding model.(5)Scattering of thrombin is inferior than others but it is easy hemostatic method.6.This project was useful for clinical methods hemostasis for variceal ruputure bleeding.