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Various types of hemostasis for a rat esophageal varices bleeding model

大鼠食管静脉曲张出血模型的各种止血方法

基本信息

批准号：
03670358
负责人：
INOUE Ryoichi
金额：
$ 1.34万
依托单位：
Kinki University (1992-1993)Kyoto University (1991)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

1.We set up first the rat esophageal system using the Olympus thin, rigid Selfoscope. For rat esophageal endoscopy it is suitable to use the rigid Selfoscope, SES1711D or SES1717D with 1.7 mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photograficmedium and U-matic video tape.2.Long time endoscopic examination for rat esophagus was performed by continuous air feeding by piping of rat stomach through the catheter.3.The production of esophageal varices in the rat is greater in Wistar ST rats weighing about 300g with 20-gauge partial portal vein ligation. It is efficient and economical for Wistar ST rats 2-weeks afer 20-gauge portal vein ligation to be used a rat esophageal varices model.4.The rat esophageal varices bleeding model was produced by stinging from the rat EIS tube by a 30-gauge EIS needle after intra-caudalvenous injection of 1000U heparin. The estimate of the grade of rat esophageal bleeding was decided the time from start of the bleeding to hemostasis.5.Hemostasis of various types of methods was performed using this rat esophageal varices bleeding model.(1)Endoscopic injection sclerotherapy(EIS) is the most efficient hemostatic method. For sclerosing agents, EO and AS were both effective.(2)EIS was superior than electrocoagulation and heater probe method.(3)For vasoreactive drugs, propranolol and vasopressin were both effective, but vasopressin was more effevtive than propranolol, significantlly.(4)Combination therapy with EIS and vasopressin or propranolol was not statistically significant for hemostasis of rat esophageal varices bleeding model.(5)Scattering of thrombin is inferior than others but it is easy hemostatic method.6.This project was useful for clinical methods hemostasis for variceal ruputure bleeding.

1.我们首先使用奥林巴斯薄型刚性自镜建立大鼠食管系统。对于大鼠食管内窥镜检查，适合使用直径为 1.7 mm 的硬质自检镜 SES1711D 或 SES1717D。通过使用视频内窥镜，内窥镜图像的分辨率和色彩表现明显更好。连接到Selfscope的视频内窥镜图像可以数字编码并存储在照相介质和U-matic录像带上。2.通过导管通过大鼠胃的管道连续供气来对大鼠食管进行长时间内窥镜检查。3.在体重约300g的Wistar ST大鼠中，采用20号门静脉部分结扎时，大鼠食管静脉曲张的产生更大。 Wistar ST大鼠20号门静脉结扎后2周建立大鼠食管静脉曲张模型是高效、经济的。4.尾静脉注射1000U肝素后用30号EIS针刺穿大鼠EIS管制作大鼠食管静脉曲张出血模型。以出血开始至止血的时间来判断大鼠食管出血的程度。5.利用该大鼠食管静脉曲张出血模型进行多种止血方法。(1)内镜下注射硬化剂治疗(EIS)是最有效的止血方法。对于硬化剂，EO和AS均有效。(2)EIS优于电凝和加热探针法。(3)对于血管反应性药物，普萘洛尔和加压素均有效，但加压素比普萘洛尔更有效。(4)EIS与加压素或普萘洛尔联合治疗，无统计学意义。大鼠食管静脉曲张破裂出血模型的止血。(5)凝血酶的分散性较其他方法差，但为简便的止血方法。6.本课题对临床治疗静脉曲张破裂出血的止血方法有一定的参考价值。