Treatment of esophageal varices by vasoactive drugs on rat esophageal varices model and Ito's cells.

1.采用奥林巴斯超薄硬质自视镜(A7001)建立了改良的大鼠食道内窥镜系统。在大鼠食道内窥镜检查中，宜使用其新型硬质自体内窥镜A7001，内径1.2 mm。通过使用视频内窥镜，内窥镜图像的分辨率和色彩性能明显更好。2.用Macintosh8600/250和Photoshop ver3.OJ(Adobe Inc.)对大鼠食道静脉曲张模型进行内窥镜分级。计算方法是在内窥镜图像区域的印象区(ROI)上估计静脉曲张面积。它的方法与通常使用数字平面仪的方法完全相同。3.心得安、加压素、尼拉地洛、奥曲肽对食道静脉曲张的作用与对照组比较，差异有统计学意义。其比值(V/ROI)分别为加压素=0.653，心得安=0.690，尼普拉地洛=0.793，奥曲肽=0.711，对照组=0.992。4.伊藤细胞培养采用Niki法，伊藤细胞收缩分级与食道静脉曲张相同。收缩分级用血管收缩指数(C/ROI)表示。5.内皮缩血管指数(V/ROI)为0.834。但除心得安为0.931.6外，其他血管活性药物无明显变化。结论：血管活性药物治疗食道静脉曲张疗效确切，尤其是硝普地洛或奥曲肽治疗效果更佳。

1.We set up revisedly rat esophageal endoscopic system using the Olympus thin, rigid Selfoscope (A7001). For rat esophageal endoscopy it is suitable to use its new rigid Selfoscope, A7001 with 1.2mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photografic medium and U-matic video tape.2.Endoscopic grading of esophageal varices of rat model was performed by using personal computer (Macintosh, 8600/250) and Photoshop ver3.OJ (Adobe Inc.). The method of calculating is estimated variceal area on region of impression (ROI) of endoscopic image field. Its method was exactrly same to the ordinally one using digital planimetor. The grade of varices was indicated by Ratio (V/ROI).3.The effect of various vasoactive drugs esophageal varices was significantlly shown by propranolol, vasopressin, nipradilol and octreotide compared with controls. The ratio of Ratio (V/ROI) was vasopressin=0.653, propranolol=0.690, nipradilol=0.793, octreotide=0.711 and control=0.992, respectively.4.The culture of Ito's cell was performed by Niki's method, The grading of contraction of Ito's cells was done the same method of that of esophageal varices. Its grading of contraction indicated by Ratio (C/ROI).5.The ratio of Ratio (V/ROI) on endothelin was 0.834. But various vasoactive drugs was not change ratio except that of propranolol=0.931.6.In conclusion, the treatment of esophageal varices with vasoactive drugs was usefull and effective, especially using nipradilol or octreotide.