权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Treatment of esophageal varices by vasoactive drugs on rat esophageal varices model and Ito's cells.

血管活性药物对大鼠食管静脉曲张模型和伊藤氏细胞治疗食管静脉曲张的影响。

基本信息

批准号：
07670631
负责人：
INOUE Ryoichi
金额：
$ 1.34万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670631/
关键词：
Rat esophageal varices model Endoscopic grading of esophageal varices using personal computer Ito's cells vasoactive drugs vasopressin propranolol nipradilol octreotide バソプレシン食道静脈瘤薬物療法

项目摘要

1.We set up revisedly rat esophageal endoscopic system using the Olympus thin, rigid Selfoscope (A7001). For rat esophageal endoscopy it is suitable to use its new rigid Selfoscope, A7001 with 1.2mm in diameter. By using videoendoscopy, the resolution capacity and color performance of the endoscopic images is significantly better. The videoebdoscopic image linked to the Selfoscope can be digitally coded and stored on a photografic medium and U-matic video tape.2.Endoscopic grading of esophageal varices of rat model was performed by using personal computer (Macintosh, 8600/250) and Photoshop ver3.OJ (Adobe Inc.). The method of calculating is estimated variceal area on region of impression (ROI) of endoscopic image field. Its method was exactrly same to the ordinally one using digital planimetor. The grade of varices was indicated by Ratio (V/ROI).3.The effect of various vasoactive drugs esophageal varices was significantlly shown by propranolol, vasopressin, nipradilol and octreotide compared with controls. The ratio of Ratio (V/ROI) was vasopressin=0.653, propranolol=0.690, nipradilol=0.793, octreotide=0.711 and control=0.992, respectively.4.The culture of Ito's cell was performed by Niki's method, The grading of contraction of Ito's cells was done the same method of that of esophageal varices. Its grading of contraction indicated by Ratio (C/ROI).5.The ratio of Ratio (V/ROI) on endothelin was 0.834. But various vasoactive drugs was not change ratio except that of propranolol=0.931.6.In conclusion, the treatment of esophageal varices with vasoactive drugs was usefull and effective, especially using nipradilol or octreotide.

1.采用Olympus超薄型自聚焦显微镜（A7001），改良建立大鼠食管内窥镜系统。大鼠食管内窥镜检查宜选用直径为1.2mm的A7001硬性内镜。通过使用视频内窥镜，内窥镜图像的分辨率能力和颜色性能明显更好。2.采用Macintosh 8600/250计算机和Photoshop 3.OJ版软件对大鼠食管静脉曲张模型进行内镜分级。计算方法是在内窥镜图像场的感兴趣区域（ROI）上估计静脉曲张面积。其方法与普通数字求积仪的方法完全相同。用V/ROI比值表示曲张程度。3.各种血管活性药物对食管静脉曲张的作用以普萘洛尔、血管加压素、尼普拉地洛和奥曲肽明显。Ratio（V/ROI）分别为：加压素=0.653，普萘洛尔=0.690，尼普拉洛=0.793，奥曲肽=0.711，对照组=0.992。4. Ito细胞培养采用Niki法，Ito细胞收缩分级采用食管静脉曲张分级法。其收缩分级用比值（C/ROI）表示。5.内皮素的比值（V/ROI）为0.834。而各种血管活性药物的比值除普萘洛尔为0.93 ± 1.6外，其余均无变化。因此，血管活性药物治疗食管静脉曲张是有效的，尤其是尼普拉洛或奥曲肽。