Antitumor effects of anitiandrogen on experimental esophageal carcinoma induced by N-Amyl-N-methlnitrosamine(AMN) in rats.

抗雄激素对N-戊基-N-甲基亚硝胺（AMN）诱导的大鼠实验性食管癌的抗肿瘤作用。

• 批准号: 03670642
• 负责人: KONDOH Yasumasa
• 金额: $ 1.34万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670642/
• 关键词: androgen; flutamide; esophageal cancer; AMN; experimental esophageal cancer; 男性ホルモン

It is well known that incidences of esophageal carcinoma in men are higher than those in women. Human skin is an androgen target organ and the androgen binding capacity of human skin cytosol was measured. We speculated that androgen might play an important role in carcinogenesis of the esophageal mucosa. Male 7-week-old rats of Sprague-Dawlew(SD) strain were used. AMN was dissolved in drinking water at a concentration of 0.003% and Flutamide(SCH13521 Shering Corporation), nonsteroidal antiandrogen agent, was administered as mixed into the solid diet in a daily dose of 20mg. The esophageal tissue were prepared to examine histopathologically. The incidences of carcinoma in intact and castrated rats administered AMN with Flutamide were significantly lower than those of intact rats administered AMN alone, and the incidences of carcinoma in intact and castrated rats that were given Flutamide after administration of AMN were significantly lower than that of intact rats were not given Flutamide after administration of AMN.These findings suggest that Flutamide has antitumor effects in experimental esophageal carcinoma induced by AMN in rats.

众所周知，男性食管癌的发病率高于女性。人皮肤是雄激素的靶器官，测定了人皮肤胞液的雄激素结合能力。我们推测雄激素可能在食管粘膜癌变过程中起重要作用。使用Sprague-Dawlew（SD）系雄性7周龄大鼠。将AMN以0.003%的浓度溶解在饮用水中，并以20 mg的日剂量将非甾体抗雄激素剂Fluoride（SCH 13521 Shering Corporation）混合到固体饮食中给药。取食管组织行组织病理学检查。AMN联合Fluorescent给药的完整大鼠和去势大鼠的癌发生率显著低于单独给药AMN的完整大鼠，给药后给予氟尿嘧啶的正常大鼠和去势大鼠的癌发生率均显著低于给药后未给予氟尿嘧啶的正常大鼠，提示氟尿嘧啶对实验性食管癌有一定的抗肿瘤作用。AMN诱发大鼠肝癌。

项目成果

近藤泰理: "非ステロイド性抗アンドロゲン剤FlutamideのAMN(N-Amyl-N-methylnitrosamine)ラット実験食道癌における発癌抑制について" 第52回日本癌学会総会記事. 22 (1993)

Yasuri Kondo：“非甾体抗雄激素氟他胺对大鼠实验性食管癌中 AMN（N-戊基-N-甲基亚硝胺）的致癌作用”，日本癌症协会第 52 届年会 22 (1993)。

近藤泰理: "N-Amyl-N-methylnitrosamine(AMN)誘発ラット実験食道癌における抗アンドロゲン剤の発癌抑制効果" 第51回日本癌学会総会記事. 65 (1992)

Yasuri Kondo：“抗雄激素对 N-戊基-N-甲基亚硝胺（AMN）诱导的实验大鼠食道癌的致癌作用”，日本癌症协会第 51 届年会文章 65（1992）。

Yasumasa Kondoh, Kyoji Ogoshi, Hiroyasu Makuuchi, Tomoo Tajima and Toshio Mitomi: "Antitumor effets of antiandrogen on experimental esophageal carcinoma induced by N-Amyl-N-methl-nitrosamine(AMN) in rats." The Journal of Japan Society for Cancer Therapy.V

Yasumasa Kondoh、Kyoji Ogoshi、Hiroyasu Makuuchi、Tomoo Tajima 和 Toshio Mitomi：“抗雄激素对 N-戊基-N-甲基-亚硝胺 (AMN) 诱导的大鼠实验性食管癌的抗肿瘤作用。”

近藤 泰理: "N-Auryl-N-wethyluitrosamine(AMN)誘発ラット実験食道癌における抗アンドロゲン剤の発癌抑制効果" 日本癌学会総会記事. 51. 65- (1992)

Yasuri Kondo：“抗雄激素对 N-Auryl-N-乙基亚胺（AMN）诱导的实验性大鼠食道癌的致癌抑制作用”日本癌症协会大会文章。 51. 65-（1992）

近藤 泰理,上條 あけみ,生越 喬二,幕内 博康,田島 知郎,三富 利夫: "抗アレドロゲン剤のNーAmylーNーmethylnitrosamine(AMN)ラット実験 食道癌における治療効果について" 日本癌学会総会記事. 50. 55 (1991)

Yasuri Kondo、Akemi Kamijo、Kyoji Ikugoshi、Hiroyasu Makuuchi、Tomoro Tajima、Toshio Mitomi：“抗过敏原 N-Amy-N-甲基亚硝胺 (AMN) 对食道癌治疗效果的大鼠实验” 日本癌症大会社会文章。 50. 55 (1991)