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再潅流性肺血管障害発生初期における活性酸素の作用と白血球系細胞の役割

活性氧的作用和白细胞在再灌注诱发的肺血管疾病早期的作用

基本信息

批准号：
03670663
负责人：
蘇原泰則
金额：
$ 1.09万
依托单位：
Jichi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至无数据
项目状态：
已结题

项目摘要

研究目的:体外循環後の肺臓や心臓、冠動脈再建後の心臓、そして臓器移植後の心臓、肺臓、腎臓、肝臓などで、再潅流に起因すると思われる様々の血管傷害が発生し大きな問題となっている。本研究では、我々の開発した生体肺微小循環観察法を用い、再潅流性肺血管障害への活性酵素と白血球の関与について検討を加えた。研究方法:体重200ー300gのwistar系ラットを用い、左主肺動脈に血流遮断と開放の可能なタ-ニケイを巻き付け、左肺横隔膜部を我々の開発した生体肺微小循環観察法で観察した。次に2群の実験を行った。(1)再潅流性肺血管障害群;まず肺微小血管での白血球付着数と血漿蛋白浸出状況を観察する。次に生理的食塩水を静注し20分に渡って左肺動脈血流を遮断する。遮断解除後10分と30分に肺微小血管での白血球付着数と血漿蛋白の浸出を観察した。(2)SOD投与再潅流群;生理的食塩水の代わりsuperoxide dismutaseを1000単位投与し、あとは再潅流性肺血管障害群と同様の実験を行った。研究成績と結論:再潅流性肺血管障害群では、再潅流後時間経過に従い肺毛細血管への白血球付着数が増加し、肺細静脈からの血漿給白浸出が増加した。これに反し、SOD投与再潅流群では白血球付着はみられず、血漿蛋白の浸出もみられなかった。以上より再潅流性肺血管障害では、まず再潅流により肺微小血管に活性酵素が作られ、血管内皮が傷害され白血球の付着しやすい状況が生まれる。この際、白血球は血流が遅く血管径の細い肺毛細血管にまず付着する。付着した白血球は血管内皮を傷害し、血管傷害物質を産成放出させ、下流で血管内皮接合部の最も脆弱な肺細剤脈を傷害し血漿蛋白の浸出を起こさせる。これが再潅流性肺血管障害の発生機序であろうと考えた。

Objective: to investigate the causes of heart disease, lung disease, heart disease, liver disease and reflow after the reconstruction of coronary arteries in vitro. In this study, we began to use the method of microcirculation in vivo and lung to detect the activity of enzymes, white blood cells and blood cells in patients with pulmonary vascular disease. Methods: the body weight was 200 ~ 300g, the body weight was 200 ~ 300g, the blood flow of the left main lung was cut off, and the diaphragm of the left lung was opened. In the second half of the year, there are two groups of people. The main results were as follows: (1) reflow pulmonary vascular dysfunction group, pulmonary microvessel count, white blood cell count and blood protein leaching were observed. After 20 minutes of intravenous injection, the blood flow of the left lung was blocked by intravenous injection of secondary physiological water. Ten minutes to 30 minutes after the occlusion was lifted, the pulmonary microvessels and white blood cells were leached with blood albumin. (2) the SOD was injected into the reflux group, and the physiological superoxide dismutase 1000 position was injected into the flow pulmonary vascular disease group. The results of the study were as follows: the number of white blood cells in pulmonary capillary blood vessels was increased in the time after recirculation, and the number of white blood cells was increased after reflow. Anti-virus, SOD and reflow group, white blood cells were collected, blood protein was leached, and white blood cells were leached. The flow pulmonary vascular dysfunction, pulmonary microvascular active enzymes, vascular endothelial cells, white blood cells, blood cells, blood vessels, pulmonary microvascular active enzymes, vascular endothelium, white blood cells, blood vessels, pulmonary microvascular active enzymes, vascular endothelial cells and white blood cells were detected. The blood flow of international and white blood cells, the diameter of blood vessels, the blood vessels of lung hair, the blood vessels of lung, the blood flow of white blood cells and the blood vessels of the lungs. Pay "white blood cells" vascular endothelial damage ", vascular" harmful substances "into the release of" vascular endothelial junction ", the most vulnerable" lung cancer "," damage "blood protein" leaching ". The sequence of pathogenesis of recurrent pulmonary vascular disease was investigated.