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Immunohistochemical study of Lewis antigen in the urological malignancy

Lewis抗原在泌尿系统恶性肿瘤中的免疫组织化学研究

基本信息

批准号：
03670763
负责人：
OKADA Kiyoki
金额：
$ 1.34万
依托单位：
Nihon University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670763/
关键词：
Lewis antigen Renal cell carcinoma Bladder tumor Immunohistochemistry Lewis抗原腎組織

项目摘要

The significance of Lewis antigens has been already demonstrated in various organs by immunochemical and histochemical studies, but little is known regarding the localization of Lewis antigens in the urological tissue. Accordingly, immunohistochemical and cytochemical studies were performed to determine the degree of the expression of Lewis antigens in renal and bladder including normal and pathological conditions and to demonstrate the ultrastructural localization. The expressions of Lewis a, b, x, y were investigated by Avidin-Biotin Complex(ABC) method with the use of Lewis monoclonal antibodies.The spcimens were obtained at the urological operations of either renal or bladder extraction. Light microscopic immunohistochemical study was carried out with use of frozen sections with dry ice as well as paraffin sections fixed with formaline. Thin sections were incubated with Lewis monoclonal antibodies a, b, x, y as a first antibody, consequently then were reacted with use of ABC method … More as a second antibody. The fine structural localization of Lewis x antigen was investigated because of the high positive rate in light microscopy. For the ultracytochemical study, the tissues were prefixed with in 2% cold glutaraldehyde, embedded in paraffin. The sections of 6um in thickness were reacted with the same procedure as in light microscopy.Among 4 types of Lewis antigens, Lewis x and b were markedly present in normal renal tissue. Secretory status showed high positive rate. No Lewis antigens were observed in malignant tissues of renal cell carcinoma. In normal tissues, positive reaction products of Lewia x appeared in the renal tubular cells, especially in the apex of proximal tubule devoid of glomerular cells. The ultrastructural localizations of Lewis x was demonstrated in microvilli of proximal tubular cell. On the other hand, bladder transitional epithelia showed strongly positive reactions against Lewis a, b, x, y. They appeared to be present on the apical cell membrane as well as apical vesicles with the reaction of Lewia b. In the malignant tissues, they tended to decrease its reactivities except Lewis x. The reactions of Lewis a and b showed slightly decreased at the cell membrane, and no reaction of Lewis y was seen in the malignant tissue. However, Lewia x showed strong reaction even in the malignant diseases. According to the present investigation, Lewia antigen seemed to be disappeared in the renal tissue in accordance with the malignant transformation. The fact that Lewis x is still present in the bladder cancer would be speculated that bladder cancer epithelia still has a function similar to that of normal bladder epithelia. Less

免疫化学和组织化学研究已经证明Lewis抗原在各种器官中的重要性，但对Lewis抗原在泌尿系统组织中的定位知之甚少。应用免疫组织化学和细胞化学方法检测Lewis抗原在正常和病理状态下肾和膀胱中的表达程度，并对其超微结构进行定位。应用抗Lewis单抗，用亲和素-生物素复合体(ABC)法检测Lewis a、b、x、y的表达。用冰冻切片和福尔马林固定的石蜡切片进行光镜免疫组织化学研究。将薄片与刘易斯单抗a、b、x、y孵育为一抗，然后用abc法…反应。更多的是作为第二种抗体。由于在光镜下的高阳性率，对Lewis x抗原的精细结构定位进行了研究。在超微细胞化学研究中，组织被2%冷戊二醛预包埋，石蜡包埋。6微米厚的切片与光学显微镜反应相同。在4种Lewis抗原中，Lewis x和B在正常肾组织中明显存在。分泌状态阳性率较高。肾癌恶性组织中未见Lewis抗原表达。在正常组织中，Lewia x阳性反应产物出现在肾小管上皮细胞，尤其是近端小管尖，无肾小球细胞。近端肾小管上皮细胞微绒毛中有Lewis x的超微结构定位。另一方面，膀胱移行上皮对Lewis a、b、x、y呈强阳性反应，出现在顶端细胞膜和顶端囊泡上，呈Lewia b阳性反应。在恶性组织中，除Lewis x外，其反应性呈下降趋势，Lewis a和B在细胞膜上呈轻度减少，在恶性组织中未见Lewis y反应。然而，即使在恶性疾病中，Lewia x也表现出强烈的反应。根据目前的调查，Lewia抗原在肾组织中似乎消失了，与恶变相一致。膀胱癌组织中仍有Lewis x的存在，推测膀胱癌上皮细胞仍具有与正常膀胱上皮相似的功能。较少