Effects of inorganic ions on the detrusor contraction induced by electro field stimulation

无机离子对电场刺激逼尿肌收缩的影响

• 批准号: 03670751
• 负责人: KONDO Atsuo
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670751/
• 关键词: Intramural nerve stimulation; Detrusor contraction; Magnesium ion; Human neurogenic bladder; 利尿筋収縮; 壁在神経刺激

The effect of magnesium ions on detrusor contraction was investigated in vitro using the rat detrusor muscle strips. Detrusor contractility caused by intramural nerve stimulation increased in a magnesium-free solution but decreased in a high magnesium solution. Cumulative addition of MgCl(-)2(-) suppressed the electrically induced contractile response of the detrusor dose-dependently. Mg-ion also suppressed atropine resistant detrusor contraction in a dose-dependent manner. Mg ion suppressed the detrusor contraction induced by carbachol and potassium. The effect of Mg ion on detrusor contraction was enhanced by verapamil, but was inhibited by Bay-K8644. Procaine increased the inhibitory effect of Mg ion on detrusor contractility. However, caffeine did not modify the effect of Mg ion. These results suggest that Mg ion suppresses detrusor muscle contractility through an inhibitory mechanism acting on calcium channels in the smooth muscle cell membrane. We also investigated similary effec … More ts of Mg ion on rabbit whole bladder.The detrusor contraction induced by intramural nerve stimulation in human neurogenic bladders was investigated in comparison with control bladders. The response of neurogenic bladders induced by intramural stimulation was significantly stronger than that of control bladders. Tetrodotoxin almost completely inhibited the detrusor contraction in both groups. After application of atropine remaining contraction of neurogenic bladders was significantly greater than that of control bladders. The subthreshold KCL ehanced the detrusor contractility significantly more in control bladders compared to neurogenic bladders. The contraction of neurogenic bladders in Ca-free solution persisted longer than that of controls. In conclusion, though there was no intrinsic difference in detrusor contractility, contractile response to electrical field stimulation, atropine resistance, enhancement of response by pottasium, and contractility in Ca-free solution were significantly different between neurogenic and control bladders. Less

采用大鼠逼尿肌条体外实验研究了镁离子对逼尿肌收缩的影响。在无镁溶液中，由外壁神经刺激引起的逼尿肌收缩力增加，而在高镁溶液中则下降。MgCl(-)2（-）的累积添加抑制了逼尿肌的电诱导收缩反应。镁离子也以剂量依赖的方式抑制阿托品抗性逼尿肌收缩。Mg离子抑制氯乙醇和钾诱导的逼尿肌收缩。维拉帕米能增强Mg离子对逼尿肌收缩的影响，而Bay-K8644能抑制Mg离子对逼尿肌收缩的影响。普鲁卡因增强了Mg离子对逼尿肌收缩力的抑制作用。然而，咖啡因并没有改变镁离子的作用。这些结果表明，镁离子通过作用于平滑肌细胞膜钙通道的抑制机制抑制逼尿肌收缩力。我们还研究了Mg离子对家兔全膀胱的类似作用。研究了人神经源性膀胱内神经刺激引起的逼尿肌收缩，并与对照膀胱进行比较。神经源性膀胱对内源性刺激的反应明显强于对照膀胱。河豚毒素几乎完全抑制了两组的逼尿肌收缩。应用阿托品后，神经源性膀胱的剩余收缩明显大于对照膀胱。与神经源性膀胱相比，阈下KCL显著增强了对照膀胱的逼尿肌收缩力。无ca溶液中神经源性膀胱的收缩持续时间比对照组长。综上所述，神经源性膀胱和对照组膀胱的逼尿肌收缩力虽然没有内在差异，但对电场刺激的收缩反应、阿托品抵抗、钾对反应的增强以及无ca溶液中的收缩力在神经源性膀胱和对照组膀胱之间存在显著差异。少

项目成果

斉藤 政彦: "ヒト神経因性膀胱の筋切片を利用した壁内神経刺激に対する反応性" 日本泌尿器科学会雑誌. (1993)

Masahiko Saito：“使用人神经源性膀胱肌肉切片对壁内神经刺激的反应”，日本泌尿外科协会杂志（1993 年）。

Masahiko Saito: "Response of the human neurogenic bladder induced by intramural nerve stimulation" Japan Journal of Urology.

Masahiko Saito：“壁内神经刺激引起的人类神经源性膀胱的反应”日本泌尿学杂志。