The interaction between RHO/ROCK and NO in the urothelium and the role of muscarinic receptor subtype for detrusor contraction

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Cumulative concentration-response curves (CRCs) to cholinergic agonists (carbachol and pilocarpine) were obtained in the human and porcine detrusor tissues with and without urothelium. After the first curve, the second CRCs to cholinergic agonists were obtained in the presence of ROCK inhibitors (Y27632、fasudil). After administration ofROCK inhibitors decrease of maximum contraction (Emax) was greater in detrusor tissues with urothelium than those without urothelium. Then CRCs to carbachol were obtained in the presence of 30 μM L-NAME plus 30 μM Y-27632. There was a significant difference with regard to Emax between the control and tissues in the presence of Y-27632 and in the presence of L-NAME plus Y-27632. However no significant difference was noted between Emax in tissues with Y-27632 and in that with L-NAME plus Y-27632.