Role of vitamin D_3 on differentiation of bone marrow-derived stromal cells.

维生素D_3对骨髓基质细胞分化的作用。

• 批准号: 03670873
• 负责人: SHINKI Toshimasa
• 金额: $ 0.32万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670873/
• 关键词: adipocyte; adipogenesis; osteoporosis; phospholipids; preadipocyte; triacylglycerol; vitamin D derivatives; デキサメタゾン; 活性型ビタミンD; 脂質代謝; 骨髄間質細胞; ホスファチジルコリン

MC3T3-G2/PA6 cells established from newborn mouse calvaria are preadipocytic stromal cells, which differentiate into adipocytes in response to glucocorticoids. We examined the effects of lalpha, 25-dihydroxyvitamin D_3 [lalpha, 25(OH) _2D_3] on adipgenesis in PA6 cells. When PA6 cells were cultured with 10^<-8> M dexamethasone, adipocytes contatining oil red O-positive droplets first appeared on day 7 (3 days after confluence was attained) and the maximal synthesis of neutral lipids occurred on day 12.Simultanieous addition of lalpha, 25(OH) _2D_3 at 10^<-9> M completely blocked this dexamethasone-induced neutral lipid synthesis throughout the 14-day culture period. Dose-response studies of vitamin D_3 derivatives showed that lalpha, 25(OH) _2D_3 was the most potent in inhibiting neutral lipid synthesis in PA6 and 24, 25-dihydroxyvitamin D_3, in that order. Dexamethasone greatly enhanced incorporation of [^<14>C]-acetic acid into triglycerol in PA6 cells. The incorporation was markedly inhibited by the addition of 10^<-9> M lalpha, 25(OH)_2D_3. Instead, lalpha, 25(OH) _2D_3 greatly increased incorporation of [^<14>C]-acetic acid into phospholipids, such as phosphatidylcholine and phosphatidylthanolamine, irrespective of the presence or absence of dexamethasone. These results suggest that 1alpha, 25(OH) _2D_3 modulation of lipid metabolism in bone marrow stromal cells is receptor mediated.

MC3T3-G2/PA6细胞是一种前脂肪细胞基质细胞，在糖皮质激素的作用下向脂肪细胞分化。我们研究了α， 25-二羟基维生素D_3 [α， 25(OH) _2D_3]对PA6细胞脂变性的影响。用10^<-8> M地塞米松培养PA6细胞时，第7天（汇合后3天）首次出现含有油红o阳性液滴的脂肪细胞，第12天中性脂合成量最大。在10^<-9> M的浓度下同时添加α， 25(OH) _2D_3完全阻断了地塞米松诱导的中性脂合成。维生素D_3衍生物的剂量反应研究表明，α, 25(OH) _2D_3对PA6和24,25 -二羟基维生素D_3中性脂合成的抑制作用最强。地塞米松大大增强了PA6细胞中[^<14>C]-乙酸与甘油三酯的结合。加入10^<-9> M α, 25(OH)_2D_3可明显抑制其掺入。相反，无论是否存在地塞米松，α, 25(OH) _2D_3都大大增加了[^<14 b> C]-乙酸与磷脂（如磷脂酰胆碱和磷脂酰乙醇胺）的结合。这些结果表明1α， 25(OH) _2D_3对骨髓基质细胞脂质代谢的调节是受体介导的。

项目成果

Cheng He Jin, et al.: "lalpha, 25-Dihydroxyvitamin D_3 regulates in vivo production of the third component of complement (C3) in bone." Endocrinology. 131. 2468-2475 (1992)

Cheng He Jin 等人：“lalpha, 25-二羟基维生素 D_3 调节骨骼中补体第三种成分 (C3) 的体内产生。”

Shionome,M.: "1α,25ーDihydroxyvitamin D_3 modulation in lipid metabolism in established bone marrowーderived stromal cells,MC3T3ーG2/PA6." J.Cell.Biochem.,. (1992)

Shionome, M.：“1α,25-二羟基维生素 D_3 对已建立的骨髓来源基质细胞脂质代谢的调节，MC3T3-G2/PA6。”（J.Cell.Biochem.，1992）

Shionome,M.: "1α,25-Dihydroxyvitamin D_3 modulation inlipid metabolism in established bone marrowderived stromal cells,MC3T3-G2/PA6." J.Cell.Biochem.48. 424-430 (1992)

Shionome，M.：“1α,25-二羟基维生素 D_3 调节已建立的骨髓源性基质细胞中的脂质代谢，MC3T3-G2/PA6。”J.Cell.Biochem.424-430（1992）。

Shionome,M.: "1 ,25-Dihydroxyvitamin D_3 modulation inlipid metabolism in established bone marrow-derived stromal cells,MC3T3-G2/PA6." J.Cell.Biochem.48. 424-430 (1992)

Shionome,M.：“1,25-二羟基维生素 D_3 调节已建立的骨髓源性基质细胞 MC3T3-G2/PA6 中的脂质代谢。”

Jin,C.H.: "1α,25-Dihydroxyvitamin D_3 regulates in vivo production of the third component of complement (C3) in bone." Endocrinology. 131. 2468-2475 (1992)

Jin, C.H.：“1α,25-二羟基维生素 D_3 调节骨骼中补体第三种成分 (C3) 的体内产生。” 131. 2468-2475 (1992)