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The usefulness of rat incisor dentin in evaluating pharmacological effects on mineralization in hard tissues

大鼠切牙牙本质在评估硬组织矿化药理作用中的用途

基本信息

批准号：
03670883
负责人：
MATSUMOTO Shosei
金额：
$ 1.28万
依托单位：
Aichi-Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

We reported several experiments showing the advantages of using rat incisor dentin for examining the pharmacological and toxicological effects of drugs on mineralization in hard tissues. The degree of mineralization in the incisor dentin was evaluated by contact microradiography (CMR) and by the hematoxylin stainability of demineralized transverse sections. In some experiments, parathyroidectomized rats, in which mineralization of the incisor dentin had already been suppressed, were used to examine the promoter action of drugs on the mineralization of dentin. This action could be used as an indicator for evaluating the medicinal effect of various calcium salts, vitamin D metabolites, and parathyroid hormone on dentin mineralization. Our experimental model seemed to be suitable for pharmacologically evaluating effects upon cellular activity in dentin formation, in vivo. The degree of inhibition of mineralization in the incisor dentin of normal rats could be regarded as an indicator for evaluating the toxicity of drugs on hard tissue. To determine the site of action, drug toxicity was evaluated as described above, as well as by examining the serum calcium levels, the physicochemical actions of drugs on remineralization, and findings in vitro. Furthermore, the pharmacological effectiveness on simultaneous evaluation of the mineralization in bone and dentin was shown in ovariectomized rats. These results suggest the usefulness of dentin in studying the effects of drugs on the mineralization of a collagenous matrix.

我们报道了几个实验，显示出使用大鼠切牙牙本质的优势，以检查药物对硬组织矿化的药理学和毒理学效应。通过接触显微放射照相术（CMR）和脱矿横切面的苏木素染色评价切牙牙本质的矿化程度。在一些实验中，甲状旁腺切除大鼠，其中切牙牙本质的矿化已经被抑制，用于检查药物对牙本质矿化的促进作用。该作用可作为评价各种钙盐、维生素D代谢产物和甲状旁腺激素对牙本质矿化作用的指标。我们的实验模型似乎是合适的，以评价在牙本质形成的细胞活性的影响，在体内。正常大鼠切牙牙本质矿化抑制程度可作为评价药物对硬组织毒性的指标。为了确定作用部位，如上所述以及通过检查血清钙水平、药物对再矿化的物理化学作用和体外结果来评价药物毒性。此外，在卵巢切除大鼠中显示了同时评价骨和牙本质矿化的药理学有效性。这些结果表明，在研究药物对胶原基质矿化的影响时，牙本质是有用的。