Dentin Biomodification for Optimization of Bioadhesive Dental Restorations

牙本质生物改性优化生物粘附性牙齿修复体

• 批准号: 10874883
• 负责人: Ana Karina B Bedran-Russo
• 金额: $ 49.6万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-24 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10874883
• 关键词: 3-Dimensional; Adhesions; Affect; Anxiety; Binding; Biocompatible Materials; Biomimetics; Chronic Disease; Clinical; Complex; Composite Dental Resin; Composite Resins; Dental; Dental Care; Dental Enamel; Dental Pulp; Dental caries; Dentin; Dentistry; Development; Economics; Exposure to; Extracellular Matrix; Failure; Formulation; Foundations; Goals; Health; Health Care Costs; Homeostasis; Impairment; Incidence; Industrial Waste; Intervention; Left; Life; Ligand Binding; Ligands; Longevity; Mechanics; Mediating; Minerals; Modification; Natural Products; Oral health; Outcome; Pathologic; Patients; Peptide Hydrolases; Performance; Pharmacologic Substance; Phase; Physiological; Plant Resins; Plants; Play; Proanthocyanidins; Procedures; Process; Prognosis; Property; Research; Role; Services; Source; Standardization; Structure; Structure-Activity Relationship; Testing; Tissues; Tooth Loss; Tooth structure; Treatment Cost; Work; common treatment; composite restoration; cost; cost effective; dental resin; effectiveness study; improved; innovation; interfacial; mechanical properties; molecular shape; pain reduction; polyphenol; pre-clinical; response; restoration; restorative dentistry; seal; stereochemistry; tissue regeneration

SUMMARY The most conservative and common treatment of missing dental tissue is direct resin composite restoration. Its failure rate is high, lasting an average of 6 years. The primary reason for failure is the development of secondary caries. An estimated 50% of resin composite interventions replaces failed restorations, leading to a vicious restorative cycle with increasing complexity, poor prognosis for the tooth, and high treatment costs. Resin-based restorations rely on micro-mechanical adhesion to enamel and dentin structures. Dentin is of particular importance as it is the bulk of the tooth and tightly connected with the pulp tissue. It is well known that components of the dentin extracellular matrix play major roles in the formation and sustainability of the dentin-resin bonds. Bioinspired by natural dentin toughening mechanisms, our group identified refined mixtures and isolated proanthocyanidins (PACs), a 3D structurally diverse class of biosynthetic polyphenols that can mimic dentin natural processes. These molecules elicit enhancement to the mechanical properties and reduce matrix biodegradability, collectively termed dentin biomodification. Additionally, we have revealed that PACs can play multi-functional roles at the inherently wet dentin-resin interfaces. Therefore, PACs represent new biomaterials with promising impact in the broader field of restorative/reparative dentistry. Notably, the PAC sources of this project are renewable industrial waste and/or by-products, respectively, making them highly sustainable from both economic and environmental perspectives. The ultimate goal is to develop a mechanistically based and clinically feasible strategy to modulate permanent physico-mechanical properties of the dentin matrix, to create more stable dentin-resin bioadhesion, and thus increase the longevity of resin composite restorations. This will be accomplished by identifying features of specific molecules, ligand-PACs, that mediate stable biomodification and durable dentin-resin interfaces. More specifically, this project will define the structure activity relationships of ligand-PACs (a) to modulate the main components of dentin (extracellular matrix and mineral) sustainably; (b) to establish and optimize bioadhesion mechanisms at the dentin-PAC- resin interfaces; and (c) to tailor interfacial responses that directly affect performance and (pre-)clinical usage. The Specific Aims are: (Aim 1) Define distinct mechanisms of interactions of ligand-PACs with the extracellular matrix, mineral phase, and altered forms of dentin. (Aim 2) Elucidate and tailor ligand-PACs to produce robust biointerfaces. (Aim 3) Determine the stability of the tooth-PAC-resin interfaces in relevant microenvironments. The sustainable biomodification of the dental tissue will overcome clinical pitfalls associated with failure of tooth-resin interfaces, particularly dentin breakdown. The ultimate outcome is the development of a PAC-based intervention approach that can revolutionize dental restoration.

总结

项目成果

Unveiling structure-activity relationships of proanthocyanidins with dentin collagen.

• DOI: 10.1016/j.dental.2021.08.013
• 发表时间: 2021-11
• 期刊: Dental materials : official publication of the Academy of Dental Materials
• 作者: Reis M;Zhou B;Alania Y;Leme-Kraus AA;Jing S;McAlpine JB;Chen SN;Pauli GF;Bedran-Russo AK
• 通讯作者: Bedran-Russo AK

Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion.

• DOI: 10.1016/j.jdent.2020.103354
• 发表时间: 2020-08
• 期刊: Journal of dentistry
• 影响因子: 4.4
• 作者: Trevelin LT;Alania Y;Mathew M;Phansalkar R;Chen SN;Pauli GF;Bedran-Russo AK
• 通讯作者: Bedran-Russo AK

Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications.

• DOI: 10.1021/acsabm.0c01507
• 发表时间: 2021-03-15
• 期刊: ACS applied bio materials
• 影响因子: 4.7
• 作者: Bim-Júnior O;Curylofo-Zotti F;Reis M;Alania Y;Lisboa-Filho PN;Bedran-Russo AK
• 通讯作者: Bedran-Russo AK

B-type Proanthocyanidins with Dentin Biomodification Activity from Cocoa (Theobroma cacao).

• DOI: 10.1021/acs.jafc.2c04288
• 发表时间: 2022-10-05
• 期刊: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
• 影响因子: 6.1
• 作者: Jing, Shu-Xi;Reis, Mariana;Alania, Yvette;McAlpine, James B.;Chen, Shao-Nong;Bedran-Russo, Ana K.;Pauli, Guido F.
• 通讯作者: Pauli, Guido F.

Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin.

Galloyl基序在原核蛋白与富含胶原蛋白的牙本质的相互作用中的矛盾作用。

• DOI: 10.1002/jbm.a.37276
• 发表时间: 2022-01
• 期刊: Journal of biomedical materials research. Part A
• 作者: Alania Y;Zhou B;Reis M;Leme-Kraus AA;McAlpine JB;Chen SN;Pauli GF;Bedran-Russo AK
• 通讯作者: Bedran-Russo AK