New quantitative and qualitative evaluations on tooth mineral changes after de-and remineralization

对脱矿和再矿化后牙齿矿物质变化的新定量和定性评估

• 批准号: 03670975
• 负责人: KAWASAKI Koji
• 金额: $ 1.34万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670975/
• 关键词: Enamel; mineral; de-and remineralization; digital subtraction; hydroxyapatite; TCP; high-resolution electron microscopy; 象牙質; 脱灰; 再石灰化; 画像解析; デジタルサブトラクション; 化学分析; 歯牙; ミネラル; サブトラクション

In order to evaluate tooth mineral changes after de-and remineralization quantitatively, we developed two types of the methods. In addition by means of high-resolution electron microscopy we evaluated the changed mineral qualitatively after demineralization or laser irradiation.1)Quantitative evaluations(1)Digital subtraction methodSubtraction image between two digitized microradiograms of the same enamel specimen before and after demineralization made it possible to visualized the changed mineral area by demineralization clearly. We also evaluated the changed mineral quantitatively using regression formula between gray level and alminum thickness.(2)CCD camera methodWe developed quantitative mineral profile evaluation method using CCD camera. The microradiograms of tooth root specimen after de-and remineralization were input as a digital image through CCD camera to a computer. The gray level of each pixel was transposed to the equivalent of alminium thickness according to the regression formula between alminium thickness and its gray level. By means of this method we can show the profile of the mineral vol% in an interested area on the screen simultaneously.2)Qualitative evaluationCrystllographic analysis with high-resolution electron microscopy suggested that enamel treated by demineralization andlaser could change from hydroxyapatite to alpha-or beta-TCP(tricalciumphosphate).

为了定量评价去矿化和再矿化后牙矿物质的变化，我们开发了两种方法。此外，我们还通过高分辨电子显微镜对脱矿或激光照射后的矿物变化进行了定性评价。1）定量评价（1）数字减影法将同一釉质样品脱矿前后的两张数字化显微X线照片之间的图像进行减影，可以清楚地显示脱矿后矿物变化的区域。利用灰度值与铝厚度的回归公式对变化的矿物进行了定量评价。（2）CCD相机法我们提出了利用CCD相机进行矿物剖面定量评价的方法。将牙根标本去矿化后的显微X线照片通过CCD摄像机以数字图像形式输入计算机。根据铝膜厚度与其灰度值的回归公式，将每个像素的灰度值转换为铝膜厚度的当量值。2）定性评价高分辨电子显微镜下的晶体学分析表明，经脱矿和激光处理的牙釉质可由羟基磷灰石转变为α-或β-磷酸三钙（TCP）。

项目成果

川崎浩二: "高分解能電子顕微鏡によるレーザー被照射脱灰エナメル質の微細構造" 口腔衛生学会雑誌. 45. 103-109 (1995)

Koji Kawasaki：“使用高分辨率电子显微镜的激光照射脱矿牙釉质的精细结构”口腔健康协会杂志 45. 103-109 (1995)。

川崎 浩二,飯島 洋一,高木 興氏: "ディジタルサブトラクションによるエナメル質ミネラル量変化の定量評価" 口腔衛生学会雑誌. 41. 592-593 (1991)

Koji Kawasaki、Yoichi Iijima、Oki Takagi：“通过数字减法定量评估牙釉质矿物质含量的变化”口腔健康协会杂志 41. 592-593 (1991)。

稲葉大輔: "根面再石灰化の初期過程におけるミネラル量の変化-単一切片法と画像処理法による評価-" 口腔衛生学会雑誌. 42. 393-398 (1992)

Daisuke Inaba：“牙根表面再矿化初始过程中矿物质含量的变化 - 通过单切片法和图像处理法进行评估”口腔健康学会杂志 42. 393-398 (1992)。

Daisuke INABA: "Changes in Mineral Volume in Root Surfaces during Early Stages of Remineralization : Evaluation by a Single-section and Videodensitometric Method." J.Dent.Health. vol.42, No.3. 393-398 (1992)

Daisuke Inaba：“再矿化早期阶段根部表面矿物质量的变化：通过单断面和视频密度法进行评估。”

Koji KAWASAKI: "Ultrastructure of Lased-demineralized Enamel Visualized by High-resolution Electron Microscopy." J.Dent.Health. vol.45, No.1. 103-109 (1995)

Koji KAWASAKI：“通过高分辨率电子显微镜观察激光脱矿牙釉质的超微结构。”