Characterizatin of rdw Rat as a New Animal Model for Hereditary Dwarfism

rdw 大鼠作为遗传性侏儒症新动物模型的表征

• 批准号: 03680042
• 负责人: FURUDATE Senichi
• 金额: $ 0.9万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680042/
• 关键词: rdw rat; Dwarfism; Pituitary; Pit-l; Thyroid; Pituitary hormones; Thyroid hormones; Thyrotropin (TSH); pit-1; 侏儒症(dwarfism); mRNA; Pitー1; ラット

The rdw rat (gene symbol; rdw) with hereditary dwarfism has been shown immunohistochemically to have subnormal numbers not only of growth hormone (GH) but also of prolactin and thyrotrophin-positive cells. To characterize the dwarfism of this strain, the expression of pituitary hormone mRNAs was examined by Northern hybridization. The pituitary gland in the rdw rat expressed 30-100 times fewer GH and prolactin mRNAs than that of the normal controls, whereas mRNAs for proopiomelanocortin and the alpha subunit of rat glycoprotein hormone revealed a significant increase. There was a slight difference in rat luteinizing hormone-beta subunit and follicle-stimulating hormone-b subunit between normal and rdw rats. The suppressed expression of a pituitary-specific transcription factor, pit-1, is considered to cause hereditary dwarfism in mouse strains, Snell and Jackson, whose phenotypes resemble those of rdw rat. In this study, however, no difference in mRNA expression for pit-l was found between rdw rats and controls. This work indicates that rdw rat may not have the same genotype as the phenotypically similar dwarf mice, the snell, Jackson and Ames.

遗传性侏儒症的RDW大鼠(基因符号；RDW)免疫组织化学显示，不仅生长激素(GH)的数量低于正常，催乳素和促甲状腺激素阳性细胞的数量也低于正常。为了鉴定该品系的矮化特性，采用Northern杂交技术检测了该品系中垂体激素基因的表达。RDW大鼠脑垂体腺GH和催乳素mRNAs的表达比正常对照组减少30-100倍，而原阿片黑素皮质素和大鼠糖蛋白激素α亚基的mRNAs则显著增加。正常组和RDW组大鼠促黄体生成素-β亚基和卵泡刺激素-b亚基略有差异。垂体特异性转录因子PIT-1的抑制表达被认为是导致小鼠品系Snell和Jackson的遗传性侏儒症，它们的表型与RDW大鼠相似。然而，在本研究中，RDW大鼠和对照组之间没有发现PIT-L基因表达的差异。这项工作表明，RDW大鼠可能与表型相似的矮小鼠Snell、Jackson和Ames具有不同的基因型别。

项目成果

K.Shibayama,Y.Ohyama,M.Ono,and S.Furadate: "Expression of messenger vibonucleic acid coding for pitwitaryhormonens and pitwitaryーspecific transcription factor,Pitー1,in the pitwifary of rdw rat wita hereditary dwarfism." Journal of Endocrinology.

K. Shibayama、Y. Ohyama、M. Ono 和 S. Furadate：“编码pitwitary激素和pitwitary特异性转录因子Pit-1的信使振动核酸在rdw大鼠遗传性侏儒症的pitwifary中的表达”。内分泌学。

柴山 啓子: "遺伝性侏儒症ラット(rdw)下垂体における下垂体ホルモン並びに下垂体特異的転写因子Pit-1mRNAの発現" 日本内分泌学会雑誌. 68. 395- (1992)

Keiko Shibayama：“遗传性犯罪 (RDW) 大鼠垂体中垂体激素和垂体特异性转录因子 Pit-1 mRNA 的表达”日本内分泌学会杂志 68. 395- (1992)。