DEVELOPMENT OF HIGHLY SENSITIVE PHYTOCHROME DETERMINATION EQUIPMENT BASED ON THE FLUORESCENCE SPECTRA CHANGES CONCOMITANT WITH PHOTOTRANSFORMATION.

基于光转换荧光光谱变化的高灵敏度光敏色素测定设备的开发。

• 批准号: 03554026
• 负责人: INOUE Yasunori
• 金额: $ 8万
• 依托单位: THE SCIENCE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03554026/
• 关键词: Fluorescence spectrum; Phototransformation intermediate; Phototransformation; Phytochrome; 光反応中間体; 光変換過程

Highly sensitive phytochrome determination equipment based on the fluorescence spectra changes concomitant with phototransformation was developed in this research.All phototransformation intermediates formed at low temperature transformed to Pr by light irradiation with isosbestic point. Fluorescence spectra changes which were induced by the phototransformation of Pr to the intermediate suggested that only lumi-R which was the intermediate observed at 84K emitted fluorescence having a peak at 700nm. Other intermediates never emitted fluorescence.Newly developed microfluorometer could detect the phytochrome solution which concentration was 0.0005 in the absorbance at 666nm at -20C.Volume of sample was only 3 ul. Determination which was done at -140C improved the sensitibity to 0.0001 in the absorbance at 666nm. This limit was 10 times higher than the usual absorption method.

在这项研究中，开发了基于荧光光谱变化与光转化的荧光光谱变化相关的高度敏感的植物色素测定设备。在低温下形成的所有光转化中间体通过光照射与同性生育点转化为PR。通过PR向中间物的光转化引起的荧光光谱变化表明，只有在700nm处峰值的84K发射荧光下观察到的中间体。其他中间体从未发出荧光。新开发的微荧光仪可以检测到植物色素溶液，在-20c时，在666nm的吸光度下，浓度为0.0005。样品的量仅为3 ul。确定在-140C时完成的确定在666nm时的吸光度下将敏感性提高到0.0001。该极限是通常的吸收方法的10倍。

项目成果

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Kagawa,D.,Kadota,A.,Wada,M.: "The junction between the plasnamembrane and the Cell Wall in fern protozemal cells,asvisualized after plasmolysis,and its retendence on allays of cortical microtubule." Proto plasma. 170. 186-190 (1992)

Kakawa,D.、Kadota,A.、Wada,M.：“蕨类植物细胞质膜和细胞壁之间的连接，在质壁分离后可见，及其在皮质微管上的保留。”

Kagawa,T.: "The junction between the plasma membrane and the cell wall in fern protonemal cells,as visualized after plasmolysis,and its dependence on arrays of cortical microtubles" Protoplasma. 170. 186-190 (1992)

Kakawa,T.：“蕨类植物原细胞中质膜和细胞壁之间的连接处，在质壁分离后可见，及其对皮质微管阵列的依赖性”原生质体。

Kagawa, T., Kadota, A.& Wada, M.: "The junction between the plasma membrane and the cell wall in fern protonemal cells, as visualized after plasmolysis, and its dependence on arrays of cortical microtubules." Protoplasma. 170. 186-190 (1992)

香川，T.，角田，A.

Iwakami, S., Yoshizawa, N., Hamaguchi, H., Inoue, Y.& Manabe, K.: "Spectral properties and photoativities of intermediates in the photoconversion from the red-light absorbing to the far-red-light absorbing form of pea phytochrome." Photochem.Photobiol.(to

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