Identification of root hair inducing substance which was derived from shoot apex and analysis of the role of this substance in low pH induced root hair formatin.

茎尖来源的根毛诱导物质的鉴定并分析该物质在低pH诱导根毛形成素中的作用。

• 批准号: 14540601
• 负责人: INOUE Yasunori
• 金额: $ 2.43万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540601/
• 关键词: lettuce; low pH; root hair formation; shoot; shoot apex; unknown substance; 根毛; 根毛形成誘導物質; 酸性条件; クロロゲン酸; 生活活性物質

High density of root hairs were induced on a lettuce root when a lettuce seedling was cultured at pH 4.In this phenomenon, supply of unknown substance which should be derived form shoot apex was essential.In this study, identification and characterization of this substance was performed.At first, proper plant materials were determined.Not only young seedling but also aged grown plant contained this substance.Therefore, 1 month old grown seedlings were chosen as a starting materials.Next, extraction methods were investigated. Acetone extraction was about 30 times eWcientx to compare with water extraction.For the bioassay, tiny acrylic resin culturing wares were constructed.Activity of acetone extracts was not influenced by heat treatment(100 C, 10 mm).This results ggested that the active substance was heat stable.Result of solvent fractionation using water-ethyl acetate indicated that the substance was fractionated in water phase.Next, resultant of solvent fractionation was partially purified by C18 pre-column.When the substance was solved in pH6 water, the substance could not be adsorbed on the column.The substance solved in pH3 water could be adsorbed on the column, and eluted by 20% acetonitrile.Using anion exchange pre-column, the substance could be eluted in the acidic fraction.These results suggested that the substance should be a week organic acid.Advanced isolation of the acidic fraction by C18 column HPLC exhibited that the substance was isolated in the single peak at 18.5-21.5% acetonitrile fraction.

当在pH 4培养生菜幼苗时，在生菜根上诱导了高密度的根毛。材料。对，提取方法进行了研究。丙酮提取约为ewcientx，与水的萃取相比。对于生物测定，构造了微小的丙烯酸树脂培养工具。丙酮提取物的活性不受热处理的影响（100 c，10 mm）。结果，这种结果是，该活性物质是使用替代的替代水平的替代水平的，该替代水平的水平。通过C18预遗前，部分纯化了溶剂分级的结果。当该物质在PH6水中溶液时，该物质不能在色谱柱上吸附。在ph3水中溶液的物质可以吸附在色谱柱上，可以在乙腈前洗脱，并在乙二苯甲酸上进行洗脱，该乙烯量会在per niion Expection中降低。有机酸。通过C18列HPLC将酸性馏分分离出来，表明该物质是在18.5-21.5％乙腈馏分中在单个峰中分离出来的。

项目成果

Takahashi, H.: "Isolation and characterization of the ACC synthase genes from lettuce (Lactuca sativa L.), and the involvement in low pH-induced root hair initiation."Plant Cell Physiol.. 44(1). 62-69 (2003)

Takahashi, H.：“生​​菜 (Lactuca sativa L.) ACC 合酶基因的分离和表征，以及参与低 pH 诱导的根毛起始。”植物细胞生理学.. 44(1)。

Takahashi, H., T.Iwasa, I.Shinkawa, A.Kawahara, T.Kurusu, Y.Inoue: "Isolation and characterization of the ACC synthase genes from lettuce(Lactuca sativa L.), and the involvement in low pH-induced root hair initiation."Plant Cell Physiol.. 44(1). 62-69 (20

Takahashi, H., T.Iwasa, I.Shinkawa, A.Kawahara, T.Kurusu, Y.Inoue：“生菜 (Lactuca sativa L.) ACC 合酶基因的分离和表征，以及低 pH 值的参与

Takahashi, H.: "Randomization of cortical microtubules in root epidermal cells induces root hair initiation in lettuce (Lactuca sativa L.) seedlings."Plant Cell Physiol.. 44(3). 350-359 (2003)

Takahashi, H.：“根表皮细胞中皮质微管的随机化诱导生菜 (Lactuca sativa L.) 幼苗根毛的萌生。”植物细胞生理学.. 44(3)。

Takahashi, T. et al.: "Isolation and characterization of the ACC synthase genes from lettuce (Lactuca sativa L.), and the involvement in low pH-induced root hair initiation"Plant Cell Physiol.. 44(1). 62-69 (2003)

Takahashi, T. 等人：“生菜 (Lactuca sativa L.) 中 ACC 合酶基因的分离和表征，以及参与低 pH 诱导的根毛起始”植物细胞生理学 44(1)。

Takahashi, H., K.Hirota, A.Kawahara, E.Hayakawa, Y.Inoue: "Randomization of cortical microtubules in root epidermal cells induces root hair initiation in lettuce (Lactuca sativa L.)seedlings."Plant Cell Physiol.. 44(3). 350-359 (2003)

Takahashi, H.、K.Hirota、A.Kawahara、E.Hayakawa、Y.Inoue：“根表皮细胞皮质微管的随机化诱导生菜 (Lactuca sativa L.) 幼苗根毛的萌生。”植物细胞生理学..