Creation of human parvovirus B19 antigen for diagnostic use.

结果：在K562、KU-812-F、RO 10和JK-1等造血细胞系以及脐血和静脉血(其余成分输血)的单个核细胞中，病毒在脐带血单个核细胞中生长较好，但低于骨髓细胞。用限制性内切酶EcoR 1切割基因组DNA的两端，几乎不能在大肠杆菌中产生病毒蛋白。经间接酶联免疫吸附试验表明，新合成的5个多肽具有明显的特异性抗原性，优于Fridell报道的3293-3364和Sato报道的4106-4156。VP1区基因位置为2912-2956，VP2区为2892-2971，VP2区为4016-4081,4142-4189,4127-4201。全文正在准备中。

Aims ; To create human parvovirus B19 antigens.Methods ; Productions of virus particles in cell cultures, of antigenic protein by gene engineering, and of antigenic peptides by synthesis.Results ; Among the hematopoietic cell lines ; K562, KU-812-F, RO 10 and JK-1, and mononuclear cells in umbilical cord blood and in venous blood (rest of the component blood transfusion), virus grew better in mononuclear cells from umbilical cord blood, though less than in those from bone marrow cells. Virus protein in e.coli was hardly produced by almost full length of the genome DNA cut bilateral ends with a restriction enzyme EcoR 1. Novel five synthetic peptides were showed clear and specific antigenicity by indirect ELISA test, better than two, which were 3293 - 3364 (reported by Fridell), 4106- 4156 (reported by Sato). Gene positions of those were 2912 - 2956, 2892 - 2971 in the region of VP1, 4016 - 4081, 4142 - 4189, and 4127 - 4201 in VP2. The full paper is in preparation.