Inverted confocal-laser-scanning-microscope with an extended excitation-emission spectrum

具有扩展激发发射光谱的倒置共焦激光扫描显微镜

• 批准号: 525029482
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525029482?language=en
• 关键词: Inverted; confocal; laser; scanning; microscope

The LMU plant science program and plant genetics labs propose the acquisition of a new inverted confocal laser scanning microscope with extended wavelength excitation and emission detection capabilities. Both sections have depended on two Leica SP5 machines acquired in 2005 and 2006, respectively. Due to the lack of spare parts, Leica terminated service of SP5 machines in 2022. This decision has rendered one machine dysfunctional already. The second SP5 may follow soon. Over the last years, several research groups with heavy imaging demand have been established at the Biocenter campus at LMU Martinsried. In addition, a the DFG-funded Collaborative Research Centre TRR has added new demands for state-of-the-art in vivo imaging methods which cannot be met currently. These demands include but are not limited to studies of genetically encoded sensors. The current and future research focus of the LMU plant science unit is to study the molecular function of chloroplasts in the plant acclimation process towards changes in the environments. Therefore, sophisticated confocal imaging capabilities in close proximity to our newly constructed strictly controlled environment chambers are indispensable. The proposed microscope will include a trigger unit to allow defined applications of various environmental stress triggers e.g., high light treatments. Several groups at LMU have expressed an interest to image organisms with far-red light absorbing pigments such chlorophyll f. Thus, a setup with extended excitation-emission features is needed. Our thorough research of all equipment on campus or in close proximity has revealed the current lack of the required microscopic capabilities at LMU biology. To fill this void, our group of eight primary users have compiled a confocal setup which would ideally satisfy all current and future imaging requirements. The LMU Center for Advanced Light Microscopy (CALM) management system will guarantee flawless online equipment booking and with that optimized equipment utilization by properly trained scientists. Through the collection of user-fees we will guarantee the longevity and sustainability of the newly acquired inverted confocal laser scanning microscope with extended wavelength excitation and emission detection capabilities.

LMU植物科学计划和植物遗传学实验室提出，具有扩展波长激发和排放检测能力的新倒置共聚焦激光扫描显微镜。这两个部分均分别取决于2005年和2006年收购的两台Leica SP5机器。由于缺乏备件，Leica在2022年终止了SP5机器的服务。该决定已经使一台机器功能失调。第二个SP5很快可能会跟随。在过去的几年中，在LMU Martinsried的BieCenter校园内建立了一些具有沉重成像需求的研究小组。此外，DFG资助的协作研究中心TRR增加了对当前无法满足的体内成像方法的新需求。这些需求包括但不限于遗传编码传感器的研究。 LMU植物科学单位的当前和未来研究重点是研究植物适应过程中叶绿体的分子功能，以改变环境的变化。因此，与我们新建造的严格控制环境室的复杂共聚焦成像能力是必不可少的。所提出的显微镜将包括一个触发单元，以允许定义的各种环境应力触发器的应用，例如高光处理。 LMU的几组对具有远红色光吸收色素的图像生物表示了兴趣，例如叶绿素f。因此，需要具有扩展激发发射功能的设置。我们对校园或紧邻设备的所有设备的详尽研究表明，目前缺乏LMU生物学所需的微观能力。为了填补这一空白，我们的八个主要用户组已经编制了一个共焦设置，理想情况下可以满足当前和将来的所有成像要求。 LMU高级光学显微镜（平静）管理系统将确保完美的在线设备预订，并通过经过适当培训的科学家进行优化的设备利用。通过收集用户费用，我们将保证具有扩展的波长激发和排放检测功能的新获得的倒置共聚焦激光扫描显微镜的寿命和可持续性。