Inverted confocal-laser-scanning-microscope with an extended excitation-emission spectrum

LMU植物科学项目和植物遗传学实验室提出了一种具有扩展波长激发和发射探测能力的新型倒置共聚焦激光扫描显微镜。这两个部分都依赖于分别于2005年和2006年购买的两台徕卡SP5机器。由于缺少备件，徕卡在2022年终止了SP5机的服务。这个决定已经使一台机器失灵了。第二个SP5可能很快就会推出。在过去的几年中，在LMU Martinsried的生物中心校区建立了几个具有大量成像需求的研究小组。此外，由dfg资助的合作研究中心TRR对目前无法满足的最先进的体内成像方法提出了新的要求。这些需求包括但不限于基因编码传感器的研究。LMU植物科学单元当前和未来的研究重点是研究叶绿体在植物适应环境变化过程中的分子功能。因此，在我们新建的严格控制的环境室附近，复杂的共聚焦成像能力是必不可少的。提议的显微镜将包括一个触发单元，以允许各种环境压力触发的定义应用，例如高光处理。LMU的几个小组已经表达了对具有远红光吸收色素（如叶绿素f）的生物体成像的兴趣。因此，需要具有扩展激发发射特性的装置。我们对校园内或附近的所有设备进行了彻底的研究，发现LMU生物学目前缺乏所需的微观能力。为了填补这一空白，我们的八个主要用户小组已经编制了一个共聚焦设置，它将理想地满足所有当前和未来的成像要求。LMU高级光学显微镜中心（CALM）管理系统将保证完美的在线设备预订，并通过适当训练的科学家优化设备利用率。通过收取用户费用，我们将保证新获得的具有扩展波长激发和发射检测能力的倒置共聚焦激光扫描显微镜的使用寿命和可持续性。

The LMU plant science program and plant genetics labs propose the acquisition of a new inverted confocal laser scanning microscope with extended wavelength excitation and emission detection capabilities. Both sections have depended on two Leica SP5 machines acquired in 2005 and 2006, respectively. Due to the lack of spare parts, Leica terminated service of SP5 machines in 2022. This decision has rendered one machine dysfunctional already. The second SP5 may follow soon. Over the last years, several research groups with heavy imaging demand have been established at the Biocenter campus at LMU Martinsried. In addition, a the DFG-funded Collaborative Research Centre TRR has added new demands for state-of-the-art in vivo imaging methods which cannot be met currently. These demands include but are not limited to studies of genetically encoded sensors. The current and future research focus of the LMU plant science unit is to study the molecular function of chloroplasts in the plant acclimation process towards changes in the environments. Therefore, sophisticated confocal imaging capabilities in close proximity to our newly constructed strictly controlled environment chambers are indispensable. The proposed microscope will include a trigger unit to allow defined applications of various environmental stress triggers e.g., high light treatments. Several groups at LMU have expressed an interest to image organisms with far-red light absorbing pigments such chlorophyll f. Thus, a setup with extended excitation-emission features is needed. Our thorough research of all equipment on campus or in close proximity has revealed the current lack of the required microscopic capabilities at LMU biology. To fill this void, our group of eight primary users have compiled a confocal setup which would ideally satisfy all current and future imaging requirements. The LMU Center for Advanced Light Microscopy (CALM) management system will guarantee flawless online equipment booking and with that optimized equipment utilization by properly trained scientists. Through the collection of user-fees we will guarantee the longevity and sustainability of the newly acquired inverted confocal laser scanning microscope with extended wavelength excitation and emission detection capabilities.