Study on the determination of algal class's contributions to total-chlorophyll concentration from characteristic carotenoids of algal classes.

从藻类特征类胡萝卜素测定藻类对总叶绿素浓度的贡献的研究。

• 批准号: 03640560
• 负责人: KOHATA Kunio
• 金额: $ 1.28万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640560/
• 关键词: carotenoid; chlorophyll a; chlorophyll a contribution; high-performance liquid chromatography; HPLC; photosynthetic pigments; phytoplankton; 網別存在量; 高速液体クロマトグラフィ-

Algal photosynthetic pigments, chlorophyll (Chl) a, Chl c and carotenoids were determined by high-performance liquid chromatography (HPLC). A reversed-phase HPLC method was developed by optimizing a solvent system for the highly sensitive determination of algal pigments in marine in situ samples. The proposed HPLC system can handle injection volumes grater than 400 mul without a significant loss in the resolution between chlorophyllide a and chlorophyll c_1+c_2. The system also reduces the sample volumes required for the analysis of typical pigments of oligotrophic sea water to 500 ml. Carotenoids characteristic to algal classes such as the Bacillariophyceae, Dinophyceae, Haptophyceae, Cryptophyceae, and Raphidophyceae were analyzed by multiple regression analysis to obtain Chl a contributions of the algal classes to total Chl a. The Chl a contributions of diatoms, raphidophycean flagellates, and dinoflagellates were compared with those calculated from cell counts and observed cell sizes ; there was a good correlation for the estimated algal-classes abundances between pigment and cell count.

采用高效液相色谱法（HPLC）测定了藻类光合色素、叶绿素a、叶绿素c和类胡萝卜素的含量。通过优化溶剂体系，建立了反相高效液相色谱法测定海洋原位样品中藻色素的方法。所提出的HPLC系统可以处理大于400穆尔的进样体积，而叶绿素a和叶绿素c_1+c_2之间的分辨率没有明显的损失。该系统还将贫营养海水典型色素分析所需的样品体积减少到500 ml。利用多元回归分析方法对硅藻、甲藻、隐藻、针藻等藻类的类胡萝卜素特征进行分析，得到藻类对总叶绿素a的贡献。叶绿素a的贡献硅藻，针藻类鞭毛虫，甲藻进行了比较与那些计算从细胞计数和观察到的细胞大小，有一个很好的相关性估计藻类类丰度之间的色素和细胞计数。

项目成果

Kunio Kohata: "Highly sensitive determination of photosynthetic pigments in marine in situ samples by high-periormance liquid chromatography" Journal of Chromatography. 558. 131-140 (1991)

Kunio Kohata：“通过高效液相色谱法对海洋原位样品中的光合色素进行高灵敏度测定”《色谱杂志》。