Study on the determination of algal class's contributions to total-chlorophyll concentration from characteristic carotenoids of algal classes.

采用高效液相色谱法测定了藻类光合色素、叶绿素（Chl） a、Chl c和类胡萝卜素的含量。通过优化溶剂体系，建立了一种高灵敏度测定海洋原位样品中藻类色素的反相高效液相色谱法。所建立的高效液相色谱系统可以处理大于400 μ l的进样量，而叶绿素内酯a和叶绿素c_1+c_2的分辨率没有明显下降。该系统还将分析少营养海水中典型色素所需的样本量减少到500 ml。通过多元回归分析，分析了硅藻、甲藻、附藻、潜藻和刺藻等藻类类的类胡萝卜素特征，得到了藻类类Chl a对总Chl a的贡献。并比较由细胞计数和观察到的细胞大小计算的鞭毛藻；在色素和细胞数量之间，估计的藻类丰度有很好的相关性。

Algal photosynthetic pigments, chlorophyll (Chl) a, Chl c and carotenoids were determined by high-performance liquid chromatography (HPLC). A reversed-phase HPLC method was developed by optimizing a solvent system for the highly sensitive determination of algal pigments in marine in situ samples. The proposed HPLC system can handle injection volumes grater than 400 mul without a significant loss in the resolution between chlorophyllide a and chlorophyll c_1+c_2. The system also reduces the sample volumes required for the analysis of typical pigments of oligotrophic sea water to 500 ml. Carotenoids characteristic to algal classes such as the Bacillariophyceae, Dinophyceae, Haptophyceae, Cryptophyceae, and Raphidophyceae were analyzed by multiple regression analysis to obtain Chl a contributions of the algal classes to total Chl a. The Chl a contributions of diatoms, raphidophycean flagellates, and dinoflagellates were compared with those calculated from cell counts and observed cell sizes ; there was a good correlation for the estimated algal-classes abundances between pigment and cell count.