Studies on the structure and function of photosystem I reaction center complex

光系统I反应中心复合体的结构与功能研究

• 批准号: 03640567
• 负责人: HOSHINA Satoshi
• 金额: $ 1.28万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640567/
• 关键词: Cross-linking; Iron-sulfur center; P700; Photoinhibition; Photosystem I; Polypeptide; Reaction center; Trypsin; 活性酵素; クロロフィル; 閃光分光; ESRスペクトル; 鉄ー硫黄クラスタ-; 熱; エチレングリコ-ル処理

1. Photosystem I particles (PSI-200) prepared by the solubilization of spinach thylakoids with Triton X-100 have been treated with a cross-linker, dithiobis(succinimidylpropionate) (DSP) which reacts specifically with amino groups. The cross-linking with DSP is concluded to give a simple method for stabilization of the iron-sulfur centers A and B in PSI-200.2. Irradiation of PSI-200 with high intensity of white light caused a decrease of the electron transport activity from DCIPH_2 to methylviologen and an irreversible bleaching of chlorophyll. The content of P700 was not affected by this treatment. No photoinhibition was, however, shown in PSI complex (Chl/P700 = 40) which was prepared by the solubilization of PSI-200 with Triton X-100, followed by column chromatography on hydroxyl-apatite gel. The column chromatography may give the simple method of PSI preparation for studies on the primary photochemistry.3. PSI reaction center complex was prepared by the heat-treatment of PSI-200 in the presence of ethylene glycol. The complex was mainly composed with three proteins, PSI-A, PSI-B and PSI-K. The trypsin-treatment of the complex reconstituted into liposomes resulted in the formation of three chlorophyll-protein bands with smaller molecular weight than that of the reaction center complex. Two of them contained P700 and A_1. Characterization of the bands thus obtained is now under investigation.

1. 用 Triton X-100 溶解菠菜类囊体制备的光系统 I 颗粒 (PSI-200) 已用交联剂二硫代双(琥珀酰亚胺基丙酸酯) (DSP) 处理，该交联剂可与氨基发生特异性反应。通过 DSP 交联得出了一种稳定 PSI-200.2 中铁硫中心 A 和 B 的简单方法。高强度白光照射 PSI-200 导致从 DCIPH_2 到甲基紫精的电子传递活性降低，并导致叶绿素不可逆漂白。 P700的含量不受该处理的影响。然而，PSI 复合物（Chl/P700 = 40）中没有显示出光抑制作用，该复合物是通过用 Triton X-100 溶解 PSI-200，然后在羟基磷灰石凝胶上进行柱色谱来制备的。柱层析可为初级光化学研究提供简单的PSI制备方法。 3． PSI反应中心复合物是通过在乙二醇存在下对PSI-200进行热处理来制备的。该复合物主要由PSI-A、PSI-B和PSI-K三种蛋白组成。重组脂质体复合物的胰蛋白酶处理导致形成三个叶绿素蛋白带，其分子量比反应中心复合物的分子量更小。其中两个包含 P700 和 A_1。目前正在研究由此获得的条带的表征。

项目成果

Watanabe,S., Tamai,N., Hoshina,S., Sanada,Y., Nishida,K. and Wada,K.: "The respiration dependent malate accumulation in leaves of a crassulacean acid metabolism plant, Kalanchoe daigremontiana Hamet et Perr." J. Plant Physiol.140. 129-133 (1992)

渡边，S.，玉井，N.，星名，S.，真田，Y.，西田，K.

Hoshina,S.and Itoh,S.: "Photosystem I reaction center in oxygenic photosynthetic organisms:Current vicws and future.in Photosynthesis:Photorcactions to Plant Productivity" Oxford & IBH Publishing Co.(eds.,Y.P.Abrol,P.Mohanty and Govindjee), 32 (1993)

Hoshina,S. 和 Itoh,S.：“含氧光合生物中的光系统 I 反应中心：光合作用的当前现状和未来：光反应对植物生产力的影响”牛津

Hoshina,S., Sue,S., Wada,K., Enami,I. and Itoh,S.: "Stabilization of iron-sulfur centers A and B in photosystem I particles by chemical cross-linking." Research in Photosynthesis (ed. N.Murata). (1993)

星名，S.，苏，S.，和田，K.，江波，I。

Watanabe,S.,Tamai,N.,Hoshina,S.,Sanada,Y.,Nishida,K.and Wada,K.: "The respiration dependent malate accumulation in lcaves of a crassulaccan acid mctabolism plant,Kalanchoe daigremontiana Hamet et Perr." J.Plant Physiol.140. 129-133 (1992)

Watanabe，S.，Tamai，N.，Hoshina，S.，Sanada，Y.，Nishida，K. 和 Wada，K.：“景天酸代谢植物 Kalanchoe daigremontiana Hamet et Perr 的洞穴中呼吸依赖性苹果酸积累

Hoshina,S.and Itoh,S.: "Photosystem I reaction center in oxygenic photosynthetic organisms:Current views and future. in Photosyntesis:Photoreactions to Plant Productivity" Oxford & IBH Publishing Co.(eds.,Y.P.Abrol,P.Mohanty and Govindjee), 32 (1993)

Hoshina,S. 和 Itoh,S.：“含氧光合生物中的光系统 I 反应中心：当前观点和未来。光合作用：光反应对植物生产力的影响” 牛津