Studies on the Structure and Function of Photosystem I Reaction Center Complex.

光系统I反应中心复合体结构与功能的研究。

• 批准号: 05640728
• 负责人: HOSHINA Satoshi
• 金额: $ 1.41万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640728/
• 关键词: alpha-helix; Amino acid sequence; Femtosecond spectroscopy; Photoinhibition; Photosystem I; Plastocyanin; Reaction center; Trypsin; α‐ヘリックス; P700; 活性酸素; トリプシン

Structure and function of photosystem I (PSI) complexes from spinach thylakoid membranes have been studied.1. Photoinhibitory illumination of PSI complexes caused an inactivation of the secondary electron acceptor, Al and the degradation of the reaction center proteins with no loss of the primary donor, P700.2. To investigate the interaction between plastocyanin and PSI complex, chemical cross-linking technique with EDC,and immunostaining with anti-PSI-F-and antiplastocyain antisera were used for a probe of their accessibility. The data suggest the presence of two plastocyanin binding sites on PSI-F subunit.3. Femtosecond transient absorption spectroscopy has been used to investigate the primary energy and electron transfer processes in PSI complexes from spinach, green algae and cyanobacterria.4. PSI reaction center (RC) complex reconstituted into phosphatidylcholine liposomes was treated with trypsin and the polypeptide fragments, thus obtauned, were analyzed by amino acid sequencer. The results support the new folding model for PSI RC proteins proposed from X-ray crystallography.

研究了菠菜类囊体膜光系统I（PSI）复合物的结构和功能. PSI复合物的光抑制照明引起的二次电子受体，铝的失活和降解的反应中心蛋白质没有损失的主要供体，P700.2。为了研究质体蓝素与PSI复合物之间的相互作用，采用EDC化学交联技术和抗PSI-F-和抗质体蓝素抗血清的免疫染色来探测它们的可及性。这些数据表明PSI-F亚基上存在两个质体蓝素结合位点.利用飞秒瞬态吸收光谱研究了菠菜、绿色藻类和蓝藻PSI复合物中的初级能量和电子转移过程.用胰蛋白酶处理PSI反应中心（RC）复合物与磷脂酰胆碱脂质体的复合物，所得多肽片段用氨基酸序列分析仪分析。这些结果支持了X射线晶体学提出的PSI RC蛋白质的新折叠模型。

项目成果

Hoshina,S.and Itoh,S.: "Photosystem I reaction center in oxygenic photosynthetic organisms:Current views and the future.in Photosynthesis Photoreactions to Plant Productivity." Oxford & IBH Publishing Co.,New Delhi.(Eds.,Y.P.Abrol,P.Mohanty & Govindjee),

Hoshina,S. 和 Itoh,S.：“含氧光合生物中的光系统 I 反应中心：光合作用光反应对植物生产力的当前观点和未来。”