Molecular interactions between microfibrils and basement membranes

微纤维与基底膜的锚定是多细胞生物的普遍原理。这一建议的具体主题集中在微原纤维-基底膜相互作用的分子机制上。我们提出了以下方法：1.用来自重组或组织来源的高纯度蛋白来检测微纤维和基底膜蛋白之间的配基结合。2.为了检测“1”未涉及的蛋白质相互作用，我们将开发一种新的表面展示技术，并将其用于筛选微纤维和基底膜蛋白之间的配体结合作用。将开发一个带有人类晶状体胶囊的模型系统。该系统使我们能够测试基底膜内微纤维的锚定和生长情况。从这些项目中，我们期望对(I)了解基底膜与其环境的功能关系，(Ii)组织工程中的新策略(例如，血管和皮肤替代物)，以及(Iii)以分散的微纤维-基底膜界面为特征的疾病的治疗概念的发展做出重要贡献。

Anchorage of microfibrils to basement membranes is a universal principle of multicellular organisms. The specific subjects of this proposal focus on molecular mechanisms of microfibril-basement membrane interactions. We propose the following approaches:1. Ligand binding between microfibrillar and basement membrane proteins will be examined with highly purified proteins from either recombinant or tissue sources. 2. To detect protein interactions not covered under "1", we will develop a new surface display technology and use it to screen for ligand binding interactions between microfibrillar and basement membrane proteins.3. A model system with human lens capsules will be developed. This system allows us to test anchorage and growth of microfibrils within basement membranes.4. Electron microscopic characterization of the microfibril-basement membrane interface.From these projects, we expect important contributions for (i) the understanding of functional relationships of basement membranes with their environment, (ii) new strategies in tissue engineering (e.g. blood vessels and skin equivalents), and (iii) the development of therapeutic concepts for diseases characterized by distrubed microfibrils-basement membrane interfaces.