Molecular interactions between microfibrils and basement membranes

微原纤维和基底膜之间的分子相互作用

• 批准号: 5250556
• 负责人: Dr. Dieter Reinhardt
• 金额: --
• 依托单位: Department of Anatomy an Cell Biology
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5250556?language=en
• 关键词: Molecular; interactions; between; microfibrils; basement

Anchorage of microfibrils to basement membranes is a universal principle of multicellular organisms. The specific subjects of this proposal focus on molecular mechanisms of microfibril-basement membrane interactions. We propose the following approaches:1. Ligand binding between microfibrillar and basement membrane proteins will be examined with highly purified proteins from either recombinant or tissue sources. 2. To detect protein interactions not covered under "1", we will develop a new surface display technology and use it to screen for ligand binding interactions between microfibrillar and basement membrane proteins.3. A model system with human lens capsules will be developed. This system allows us to test anchorage and growth of microfibrils within basement membranes.4. Electron microscopic characterization of the microfibril-basement membrane interface.From these projects, we expect important contributions for (i) the understanding of functional relationships of basement membranes with their environment, (ii) new strategies in tissue engineering (e.g. blood vessels and skin equivalents), and (iii) the development of therapeutic concepts for diseases characterized by distrubed microfibrils-basement membrane interfaces.

微纤维在基底膜上的锚定是多细胞生物的普遍原理。该提案的具体主题集中在微纤维基底膜相互作用的分子机制。我们提出以下方法：1.微纤维和基底膜蛋白之间的配体结合将用来自重组或组织来源的高度纯化的蛋白质进行检查。2.为了检测“1”中未涵盖的蛋白质相互作用，我们将开发一种新的表面展示技术，并利用它来筛选微纤维和基底膜蛋白之间的配体结合相互作用.将开发具有人类透镜胶囊的模型系统。该系统允许我们测试微纤维在基底膜内的锚定和生长。微纤维-基底膜界面的电子显微镜表征。从这些项目中，我们期望对（i）基底膜与其环境的功能关系的理解，（ii）组织工程（例如血管和皮肤等效物）的新策略，以及（iii）以微纤维-基底膜界面紊乱为特征的疾病的治疗概念的发展做出重要贡献。