Induction of the polyploid silkworms, Bombyx mori, and their cytogenetical analysis

多倍体家蚕 Bombyx mori 的诱导及其细胞遗传学分析

• 批准号: 04304015
• 负责人: OSHIKI Toshikazu
• 金额: $ 9.6万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304015/
• 关键词: Polyploid; triploid; tetraploid; hexaploid; heteroploid; chromosome; silkworm; 単為発生; 還元分裂; 混数体; 家蚕; 性クロマチン; 性染色体構成; 不還元型卵核; 雌核発生; 胚致死; 胚子; 不妊

Indction of polyploidy : Triploid, tetraploid and hexaploid silkworms were induced by the high-or low-temperature treatment to the inseminated eggs immediately after oviposition. The uncleus (ZW) of the diploid egg uncleus fused with the pronucleus (Z) of the haploid sperm leading to the formation of triploid female zyogote, and hexaploid silkworm was for the duplication of ZZW triploid zyogote. Disjunction of chromosomes : Triploid progenies whith hatched from one batch oviposited by tetraploid female crossed with a diploid male were indicated the abnormal segregation of sex or the blood color. These results suggested that non-disjunction of sex-chromosomes and autosomes occurred in the tetraploid oocyte of the tetraploid silkworm. Embryonic death : Some eggs oviposited by a triploid female crossed with a diploid were ferilized, none of the fertilized eggs hatched out. The embryos in these eggs were heteroploid (2.5n). Histological observation of the embryogenesis revealed of the presence of severe abnormalities and the eggs eventuelly died during subsequent embryonic development.

多倍性指数：用产卵后立即进行高温或低温处理的受精卵，诱导出三倍体、四倍体和六倍体家蚕。二倍体卵核的核（ZW）与单倍体精子的原核（Z）融合，形成三倍体雌性合子，六倍体家蚕是为了复制ZZW三倍体合子。染色体分离：四倍体雌虫与二倍体雄虫杂交，一次产卵孵化出的三倍体后代，其性别分离或血液颜色异常。这些结果表明，四倍体家蚕卵母细胞中存在性染色体和常染色体的不分离现象。胚胎死亡：三倍体雌虫与二倍体雌虫杂交产生的部分卵受精，无受精卵孵化。这些卵中的胚胎为异倍体（2.5n）。胚胎发生的组织学观察显示存在严重的异常，并且卵最终在随后的胚胎发育过程中死亡。

项目成果

小林 正彦: "ガットウルガイランド合意後の蚕糸業への提言-明日のための蚕糸学研究21GC08:蚕糸科学と技術" 34. 14-17 (1994)

小林正彦：《Gat乌鲁盖土地协议后对丝绸业的建议 - 明天的蚕业研究21GC08：蚕业科学与技术》34. 14-17 (1994)

T.Narumi: "The fine structure of cocoon filament in wild silkmoth,Loepa katinka sakaei Inoue" Int.J.Wild Silkmoth and Silk. 1. 22-25 (1994)

T.Narumi：“野蚕蛾茧丝的精细结构，Loepa katinka sakaei Inoue” Int.J.野蚕蛾和丝绸。

A.Promboom: "Randon amplified polymorphic DNA(RAPDs)in silkmoths" Int.J.Wild Silkmoth and Silk. 1. 171-176 (1994)

A.Promboom：“蚕蛾中兰登扩增多态性 DNA (RAPD)”Int.J.Wild Silkmoth and Silk。

K.S.Ryu: "The histopathological changes and virus mataration infat body cells in fected with Pieris rapae" J.Kor.Soc.Virology. 24. 35-42 (1994)

K.S.Ryu：“感染菜青虫的脂肪体细胞中的组织病理学变化和病毒成熟”J.Kor.Soc.Virology。

野口 洋子: "ポリメラーゼ連鎖反応による飼育残沙を含めた大量試料からの核多角体病蚕の検出" 日本蚕糸学雑誌. 63. 399-406 (1994)

Yoko Noguchi：“通过聚合酶链反应从大样本中检测核多角体蚕，包括饲养残留物”日本血清学杂志 63. 399-406 (1994)。