THE CROSS TALK OF SIGNAL TRANSMISSION BETWEEN THE RECEPTOR AND THE CHANNEL

接收器与通道之间信号传输的串扰

• 批准号: 04304028
• 负责人: AKAIKE Norio
• 金额: $ 3.2万
• 依托单位: KYUSYU UNIVERSITY (1993)Tohoku University (1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304028/
• 关键词: Receptor; Ion Channel; Hydrolysis of membrane lipid; Cross talk; Second messenger; Intracellular calcium; Phosphorilation of protein; mRNA; cDNA; 生体電気シグナル; 神経細胞

The second messenger system of the metabotropic glutamate, serotonin_2, TRH and muscarinic_3 receptors-induced responses and relationship among them was investigated. It was suggested that neurons selects and integrates the inputs from different path ways at the 2nd messenger level (Akaike). The excitability of neurons were strongly depended upon the glucose metabolism (Akasu). Depolarization at no-oxygen and no-glucose condition was caused by the increase of Ca^<2+>-dependent cation permeability. Hyperpolarization at the low oxygen condition was cased by activation of Ca^<2+>/CaM kinase II and C kinase (Higashi). Synthesis of cyclic ADP-ribose was involved in the Ca^<2+>-induced Ca^<2+> release and new mechanism of that from mitochondria were also found (Kuba). Intracellular Na^+ regulate the intracellular Ca^<2+> concentration (Sorimachi). Ca^<2+> binds to CaM which activates the myosin light chain kinase. This seems to be involved in the presynaptic release of neurotransmitterrs (Kobayashi). The warm-sensitive neurons of thermoregulation center induced Na^+ current passed through "sleepy" like channel at the high temperature. PC-12 cells seems to be useful model for the study of central cool-sensitive neurons (Kiyohara). It was also studied that the process of initial development of nervous system (Takahashi), voltage-dependent intracellular Ca^<2+> release mechanism (Inoue), the role of ion channel for digestion and absorption (Okada, Kitazato), modulation of axonal flow by second messenger (Takenaka), the automatic contraction of stomach smooth muscle (Tomita), endothelial cell dependent hyperpolarization of vascular smooth muscle (Suzuki), Cl^- of squid (Yamagishi), Na^+ channel (Seyama, Noma) and gap junction (Tonosaki).

本文研究了代谢型谷氨酸受体、促肾上腺皮质激素受体_2、促甲状腺激素释放激素受体和毒蕈碱受体_3诱导反应的第二信使系统及其相互关系。这表明神经元在第二信使水平（Akaike）选择和整合来自不同路径的输入。神经元的兴奋性强烈依赖于葡萄糖代谢（Akasu）。无氧无糖条件下的去极化是由Ca^<2+>依赖性阳离子通透性增加引起的。低氧条件下的超极化由Ca^<2+>/CaM激酶II和C激酶（Higashi）的激活引起。环ADP核糖的合成参与了Ca^<2+>诱导的Ca^<2 +>释放，并发现了线粒体释放Ca ^<2 +>的新机制（Kuba）。细胞内Na^+调节细胞内Ca^<2+>浓度（Sorimachi）。Ca^<2+>与激活肌球蛋白轻链激酶的CaM结合。这似乎与神经递质的突触前释放有关（小林）。体温调节中枢的温敏神经元在高温下通过“睡眠”样通道诱发Na^+电流。PC-12细胞似乎是研究中枢冷敏感神经元的有用模型（Kiyohara）。研究了神经系统发育的初始过程（高桥），电压依赖性细胞内Ca^<2+>释放机制（井上），离子通道对消化和吸收的作用（Okada，Kitazato），第二信使对轴突流的调节（Takenaka），胃平滑肌的自动收缩（Tomita），血管平滑肌的内皮细胞依赖性超极化（Suzuki），鱿鱼（Yamagishi）的Cl^-、Na^+通道（Seyama、Noma）和缝隙连接（Tonosaki）。

项目成果

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Kobayashi,H.：“缓慢突触事件的细胞内转导机制”Can.J.Physiol.Pharmacol.70。

Ebihara, S.: "Thyrotropin-releasing hormone operated potassium currents in dissociated CA1 pyramidal neurons of rat hippocampus" J.Physiol.472. 689-710 (1993)

Ebihara, S.：“促甲状腺素释放激素控制大鼠海马 CA1 锥体神经元中的钾电流”J.Physiol.472。

Matsuda, T.: "Thermal and PGE2 sensitivity of the organum vasculosum lamina teminalis region and preoptic area in the rat brain slices" J.Physiol.454. 197-212 (1992)

Matsuda, T.：“大鼠脑切片中血管器终层区域和视前区域的热和 PGE2 敏感性”J.Physiol.454。

Iwasa,J.: "Synthesis of the photoaffinity-labeled grayanotoxins for studying the gating mechanism of Na channel" Biosci.Biotech.Biochem.56. 1169-1171 (1992)

Iwasa，J.：“用于研究Na通道门控机制的光亲和标记的灰藻毒素的合成”Biosci.Biotech.Biochem.56。

Ebihara,S.: "Thyrotropin-releasing hormone operated potassium currents in dissociated CAl pyramidal neurones of rat hippocampus" J.Physiol.472. 689-710 (1993)

Ebihara,S.：“促甲状腺激素释放激素在大鼠海马分离的 CA1 锥体神经元中控制钾电流”J.Physiol.472。