Changes of excitatory and inhibitory amino acid responses in the relay neurons of lateral geniculate nucleus by optic nerve denervation

视神经去神经后外侧膝状核中继神经元兴奋性和抑制性氨基酸反应的变化

• 批准号: 07407002
• 负责人: AKAIKE Norio
• 金额: $ 11.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407002/
• 关键词: lateral geniculate nucleus neuron; retrograde tracer DiI; excitatory amino acid response; inhibitory amino acid response; optic nerve denervation; neurotrauma; aging; intracellular chloride; シナプス・ブートン標本; 自発性シナプス後電流; 抑制性シナプス後電流; GABA; 興奮性アミノ酸受容

In order to clarify the factors contributing to the activities of dorsal lateral geniculate nucleus (LGNd) neurons, we analyzed the physiological properties of excitatory and inhibitory amino acid receptors of the neurons freshly dissociated from animals with or without optic nerve denervation.(1) The LGNd neurons identified by injection of a retrograde tracer, DiI,were prepared for the electrophysiological study using nystatin perforated patch recording mode under voltageclamp condition.(2) The effect of excitatory amino acids on the neurons was stronger in the order of KA>NMDA>AMPA>Glu.(3) A neurotransmitter candidate at retino-geniculate synapses, NAAG,mainly acted at NMDA receptors with a weak KA-like action on non-NMDA receptors.(4) The values of relative permeability of Ca^<2+> for Cs^+ (Pc_a/Pc_S) were 9.57 and 0.16 for NMDA-and KA-induced responses, respectively.(5) Intracellular C1^- concentration increased with aging : i. e. 35 mM for 0-2-day-old rat neuron, 22 mM for 2-week-old one, and 15 mM for 6-month-old one. Consequently, GABA depolarizes the neurons of immature rat.(6) Denervation induced the rise of intracellular C1^- concentration and also decreased Mg^<2+>blockade of NMDA response.(7) It was found in the study using 'synaptic bouton' preparation that NMDA,AMPA,KA receptors existed on GABAergic nerve endings. THese receptores facilitated tha GABA release from its nerve terminals. Both GABA_B and mGlu receptors also exist on GABA ergic nerve endigs and act as autoreceptors.

为了阐明外膝体背核（LGNd）神经元活动的影响因素，我们分析了视神经失神经和未失神经的动物新鲜分离的LGNd神经元兴奋性和抑制性氨基酸受体的生理特性。(1)用制霉菌素穿孔膜片记录法，在电压灯条件下，对经逆行示踪剂DiI标记的LGNd神经元进行电生理研究。(2)兴奋性氨基酸对神经元的作用顺序为KA>NMDA>AMPA>Glu。(3)视神经-膝状体突触的神经递质候选物NAAG主要作用于NMDA受体，对非NMDA受体有弱的KA样作用。(4)NMDA和KA诱导的Ca^2+对Cs^+的相对渗透率（Pc_a/Pc_S）分别为9.57和0.16。(5)细胞内C1^-浓度随衰老而增加：i. e.对于0-2天大的大鼠神经元，35 mM，对于2周龄的神经元，22 mM，对于6月龄的神经元，15 mM。结果表明，GABA使未成熟大鼠神经元去极化。(6)失神经支配引起细胞内C1^-浓度升高，也降低了Mg^<2+>对NMDA反应的阻断作用。(7)用“突触终末”法研究发现，GABA能神经末梢上存在NMDA、AMPA、KA受体。这些受体促进GABA从其神经末梢释放。GABA_（B）和mGlu受体也存在于GABA能神经末梢上，作为自身受体发挥作用。

项目成果

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Akaike, N.& Rhee, J.S.: "Age-related functional changes of the glutamate receptor channels in the rat Meynert neurons." J.Physiol.(London). 504.3. 665-681 (1997)

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