STUDIES ON DYNAMIC PROPERITIES OF CYTOSKELETAL FILAMENT SYSTEMS

细胞骨架丝系统的动力学特性研究

• 批准号: 04304057
• 负责人: MABUCHI Issei
• 金额: $ 8.32万
• 依托单位: UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04304057/
• 关键词: Cytokinesis; Action filaments; Microtubules; Intermediate filaments; Contractile ring; Mitotic apparatus; Dynein; Kinesin; 細胞接着装置; ミオシン; リン酸化

1) Cytokinesis : It has been shown that both protein phosphorylation and a low molecular weight protein rho are involved in the organization of the contractile ring. 2) In vitro motility : Tropomyosin-troponin enhances efficiency of chemical-mechanical energy transfomation in the actomyosin interaction. Kinesin head domain has been shown to be able to slide microtubules (MTs). 3) Structure-function relationship of actin : Chimera actins have been produced from Tetrahymena and Dictyostelium. A segment spanning from res. 38-83 in the actin sequence has been shown to interact with DNase I.4) Membrance-skeleton interaction : ERM family proteins are involved in constitution of the actin cytoskeleton. These proteins directly interact with the membrane protein CD44.5) Mitotic apparatus : Hexylene glycol, a MT-stabilizer, enlarges metaphase spindle at concentrations less than 3%, and slows chromosome movements. 6) Motility in chromatophores : Ca ion concentration raises prior to aggregation of pigments in melanophores. Pigments moves through interaction between motor proteins and MTs. 7) Brain MTs : An epitope for anti-tubulin antibody E3B8 is present in res. 432-452 which is common in all alpha-tubulins. 8) Dynamic properties of MTs : MTs switch from depolymerization phase to polymerization phase at the site of MAPs binding. The MT-satsbilizing ability of MAPs is abolished by phosphorylation by MPF.22S dynein binds to MTs in an ATP-sensitive manner and thereby stabilizes the MTs. 9) Intermediate filaments : Type 2A phosphatase associates with neurofilaments in the spinal cord and stabilizes the neurofilaments. It ia likely that CaM-kinase II is a intermediate filament-kinase during cell division.

1)胞质分裂：已经表明，蛋白磷酸化和低分子量蛋白rho都参与收缩环的组织。2)体外运动：原肌球蛋白-肌钙蛋白增强肌动球蛋白相互作用中化学-机械能代谢的效率。驱动蛋白头部结构域已被证明能够滑动微管（MT）。3)肌动蛋白的结构与功能关系：四膜虫和网骨藻中已经产生了嵌合肌动蛋白。已显示肌动蛋白序列中从res.38 -83跨越的片段与DNA酶I相互作用。4）膜-骨架相互作用：ERM家族蛋白参与肌动蛋白细胞骨架的构成。这些蛋白质直接与膜蛋白CD 44相互作用。5）有丝分裂装置：己二醇，一种MT稳定剂，在浓度低于3%时扩大中期纺锤体，并减慢染色体运动。6)色素细胞中的运动性：在黑色素细胞中的色素聚集之前，钙离子浓度升高。色素通过马达蛋白和MT之间的相互作用移动。7)脑MT：抗微管蛋白抗体E3 B8的表位存在于res.432 -452中，其在所有α-微管蛋白中是常见的。8)MT的动态特性：MT在MAPs结合位点从解聚阶段切换到聚合阶段。MPF.22S动力蛋白以ATP敏感的方式与MT结合，从而稳定MT。9)中间丝：2A型磷酸酶与脊髓中的神经丝结合并稳定神经丝。CaM-激酶II可能是细胞分裂过程中的一种中间激酶。

项目成果

Y.Matsuoka: "Two different protein kinases act on a different time schedule as glial filament kinase during mitosis" EMBP Journal. 11. 2895-2902 (1992)

Y.Matsuoka：“两种不同的蛋白激酶在有丝分裂过程中以不同的时间安排发挥作用，就像神经胶质丝激酶一样”EMBP 杂志。

Issei Mabuchi: Springer-Verlag, 264 (1992)

马渊一诚：施普林格出版社，264 (1992)

Mabuchi,I.(執筆分担): "Guidebook to the Cytoskeletal and Motor Proteins" Oxford University Press, 296 (1993)

Mabuchi, I.（合著者）：《细胞骨架和运动蛋白指南》牛津大学出版社，296 (1993)

Mabuchi,I.et al.: "A rho-like protein is involved in the organization of the contractile ring in dividing sound dollar eggs." Zygote. 1(印刷中). (1993)

Mabuchi, I. 等人：“一种 rho 样蛋白参与了分裂健全美元卵的收缩环的组织。”（正在出版）。

Naruki Sato: "A gene family consisting of ezrin,radixin,and moesin. Its specific localization at actin/plasma membrune assoiation sites." Journal of Cell Science. 103. 131-143 (1992)

Naruki Sato：“由 ezrin、radixin 和 moesin 组成的基因家族。其特定定位于肌动蛋白/质膜关联位点。”