Molecular mechanism that induces cytokinesis

诱导胞质分裂的分子机制

• 批准号: 19370080
• 负责人: MABUCHI Issei
• 金额: $ 9.4万
• 依托单位: Gakushuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19370080/
• 关键词: 遺伝子; 細胞・組織; シグナル伝達; 生体分子; 蛋白質; 細胞; タンパク質; 電子顕微鏡; 細胞質分裂; 収縮環; アクチンフィラメント; 細胞分裂; アクチン; ミオシン; 細胞骨格; アクチン調節タンパク質; 分裂シグナル

Actin was purified from fission yeast cells for the first time, and it was shown to polymerize into filaments that are indistinguishable from muscle actin filaments. The contractile ring actin filaments were visualized and their directionality was determined. Filaments were shown to shorten during contraction of the ring. We proposed that actin first polymerizes at one point in the division plane to both directions, and then the directionality was mixed in the ring prior to contraction. Using cultured fly cells we showed that myosin molecules in the cytoplasm accumulate at the division cortex during cytokinesis.

首次从分裂酵母细胞中纯化肌动蛋白，并证明其聚合成细丝，与肌动蛋白细丝没有区别。收缩环肌动蛋白丝可见并确定其方向性。细丝在环收缩过程中缩短。我们提出肌动蛋白首先在分裂平面上的一点向两个方向聚合，然后在收缩之前将方向性混合在环中。利用培养的蝇细胞，我们发现细胞质中的肌球蛋白分子在细胞分裂过程中聚集在分裂皮层。

项目成果

In vivo phosphorylation of regulatory light chain of myosin II in sea urchin eggs and its role in controlling myosin localization and function during cytokinesis.

• DOI: 10.1002/cm.20246
• 发表时间: 2008-02
• 期刊: Cell motility and the cytoskeleton
• 作者: Ryota Uehara;H. Hosoya;I. Mabuchi

Calyculin-A induces cleavage in a random plane in unfertilized sea urchin eggs

Calyculin-A 在未受精的海胆卵中诱导随机平面的裂解

• 发表时间: 2009
• 期刊: Biological Bulletin 216
• 作者: Fujimoto;H.;太田智彦;Makoto Goda
• 通讯作者: Makoto Goda

Formation and structure of the contractile ring in fission yeast and animal cells

裂殖酵母和动物细胞中收缩环的形成和结构

• 发表时间: 2008
• 作者: Kashiwazaki;J.;Issei Mabuchi;Issei Mabuchi;馬渕一誠;Yukako Shirato;Yuka Oiwa;Issei Mabuchi
• 通讯作者: Issei Mabuchi

酵母細胞のアクチン細胞骨格

酵母细胞的肌动蛋白细胞骨架

• 发表时间: 2007
• 作者: 高木智子;青山一弘;馬渕一誠;大隅正子
• 通讯作者: 大隅正子

Roles of Midl/Dmfl and Plol in formation of the contractile ring in fission yeast.

Midl/Dmfl 和 Plol 在裂殖酵母收缩环形成中的作用。

• 发表时间: 2008
• 作者: Horita;Y.;Ohashi;K.,and Mizuno;K.;Yukako Shirato
• 通讯作者: Yukako Shirato