Studies on te regulatory mechanisms of gene expression in early embryos

早期胚胎基因表达调控机制的研究

• 批准号: 04404016
• 负责人: TOYODA Yutaka
• 金额: $ 11.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404016/
• 关键词: oocyte maturation; fertilization; cyclin; acrosin; endothelin; parthenogenesis; chimeras; adenovirus; 初期胚; 遺伝子発現; 性分化; 体外受精; 性判別; 卵丘; 減数分裂; レポーター遺伝子; 胚培養; 顕微注入; メタロチオネイン; ベータガラクトシダーゼ

1) Gene expression during oocyte maturationA) Maturation medium influences the fluctuation pattern of H1K activity and the biological characteristics of porcine oocytes cultured in vitro.B) Oocyte-cumulus cell complexes influence the structure of the oviductal wall during the passage in the oviduct.2) Gene expression during fertilizationA) Male mice homozygous for a targeted mutation in the mouse acrosin gene were still fertile.B) Supplementing the fertilization medium with soluble factor (s) vortexed out of cumulus-oocyte complexes improved the rate of fertilization when spermatozoa were preincubated in BSA-supplemented medium.C) Oocytes from c-mos-deficient mice matured to the second meiotic metaphase, but were activated without fertilization.3) Gene expression during early embryogenesisA) A steroid, progesterone, was potent to induce parthenogenetic development of unfertilized eggs.B) ET-1^<-/-> homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs.

1)卵母细胞成熟过程中的基因表达A）成熟培养基影响体外培养的猪卵母细胞的H1 K活性波动模式和生物学特性。B）卵母细胞-卵丘细胞复合体影响在输卵管中传代过程中输卵管壁的结构。2）受精过程中的基因表达A）小鼠顶体酶基因中靶向突变的纯合子雄性小鼠仍然具有生育能力。B）当精子在补充BSA的培养基中预孵育时，用从卵丘-卵母细胞复合物中涡旋出来的可溶性因子补充受精培养基提高了受精率。C）来自c-mos缺陷小鼠的卵母细胞成熟至第二减数分裂中期，但在没有受精的情况下被激活。3）早期胚胎发生期间的基因表达A）类固醇，孕酮，B）ET-1^<-/->纯合子小鼠在出生时死于呼吸衰竭，并且具有咽弓衍生的颅面组织和器官的形态学异常。

项目成果

豊田 裕: "Sperm from mice carrying a targeted mutatiou of the acrosin gene can penetrate the oocyte 20na pellucida and ettect tertilization" Journal of Biological Chemistry. 269. 31845-31849 (1994)

Yutaka Toyoda：“携带顶体素基因定向突变的小鼠的精子可以穿透卵母细胞 20na 透明并实现受精”《生物化学杂志》269. 31845-31849 (1994)。

久和 茂: "Apoptosis induced in mouse hepatitis virusinfected cells by virus-specific CD8^+ cytotoxic T-lymphocyte clone" Journal of Virology. 68. 7540-7545 (1994)

Shigeru Hisawa：“病毒特异性 CD8^+ 细胞毒性 T 淋巴细胞克隆诱导小鼠肝炎病毒感染细胞凋亡”《病毒学杂志》68. 7540-7545 (1994)。

岩倉洋一郎: "Augmentation of c-fos and c-jun expression in tumors in transgenic mice carrying the human T-cell leukemiq virus gene" Virus Gene. (印刷中). (1995)

Yoichiro Iwakura：“携带人类 T 细胞白血病病毒基因的转基因小鼠肿瘤中 c-fos 和 c-jun 表达的增强”病毒基因（1995 年出版）。

豊田 裕: "Effcuto F priongeel sperm princabatin and elevated calaium cmcentratias on firtiligatin J cunnluo-free manoe eqqs in SEO" Trsrnal j Reprodu ction and Xevehpmenr. 38. 219-224 (1992)

Yutaka Toyoda：“SEO 中 Firtiligatin J cunnluo-free manoeeqs 的 Effcuto F priongeel 精子 princabatin 和升高的钙浓度”Trsrnal j Reproduc ction 和 Xevehpmenr 38. 219-224 (1992)。

伊庭英夫: "Analysis of AP-I tunction in cellular transformation pathways" Journal of Virology. 68. 3527-3535 (1994)

Hideo Iba：“细胞转化途径中 AP-I 功能的分析”病毒学杂志 68. 3527-3535 (1994)。