Studies on te regulatory mechanisms of gene expression in early embryos

1)卵母细胞成熟过程中的基因表达a)成熟培养基影响体外培养猪卵母细胞H1K活性的波动规律和生物学特性。B)卵母细胞-积云细胞复合物在输卵管内通过时影响输卵管壁的结构。2)受精过程中的基因表达a)与小鼠顶蛋白基因靶向突变纯合的雄性小鼠仍可生育。B)当精子在补充bsa的培养基中预孵育时，在受精培养基中添加从卵母细胞复合物中旋出的可溶性因子提高了受精率。c) c-mos缺陷小鼠的卵母细胞成熟到第二个减数分裂中期，但在没有受精的情况下被激活。A)甾体黄体酮能诱导未受精卵孤雌发育。B) ET-1^<-/->纯合子小鼠出生时死于呼吸衰竭，咽弓源性颅面组织和器官形态异常。

1) Gene expression during oocyte maturationA) Maturation medium influences the fluctuation pattern of H1K activity and the biological characteristics of porcine oocytes cultured in vitro.B) Oocyte-cumulus cell complexes influence the structure of the oviductal wall during the passage in the oviduct.2) Gene expression during fertilizationA) Male mice homozygous for a targeted mutation in the mouse acrosin gene were still fertile.B) Supplementing the fertilization medium with soluble factor (s) vortexed out of cumulus-oocyte complexes improved the rate of fertilization when spermatozoa were preincubated in BSA-supplemented medium.C) Oocytes from c-mos-deficient mice matured to the second meiotic metaphase, but were activated without fertilization.3) Gene expression during early embryogenesisA) A steroid, progesterone, was potent to induce parthenogenetic development of unfertilized eggs.B) ET-1^<-/-> homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs.