Studies on te regulatory mechanisms of gene expression in early embryos

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1) Gene expression during oocyte maturationA) Maturation medium influences the fluctuation pattern of H1K activity and the biological characteristics of porcine oocytes cultured in vitro.B) Oocyte-cumulus cell complexes influence the structure of the oviductal wall during the passage in the oviduct.2) Gene expression during fertilizationA) Male mice homozygous for a targeted mutation in the mouse acrosin gene were still fertile.B) Supplementing the fertilization medium with soluble factor (s) vortexed out of cumulus-oocyte complexes improved the rate of fertilization when spermatozoa were preincubated in BSA-supplemented medium.C) Oocytes from c-mos-deficient mice matured to the second meiotic metaphase, but were activated without fertilization.3) Gene expression during early embryogenesisA) A steroid, progesterone, was potent to induce parthenogenetic development of unfertilized eggs.B) ET-1^<-/-> homozygous mice die of respiratory failure at birth and have morphological abnormalities of the pharyngeal-arch-derived craniofacial tissues and organs.