DEVELOPMENT OF NEW SUSPENTION CULTURE MEDIUMS BY USING COLLOIDAL BUFFERS

使用胶体缓冲液开发新的悬浮培养基

基本信息

批准号：
04506001
负责人：
FUKUYAMA Toshio
金额：
$ 28.16万
依托单位：
EHIME UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04506001/
关键词：
Hydroponic solution Culture solution Tis ; sue culture Culture medium Zeolite Ion permeability Blossom-end rot Suspension culture 養液栽培法水耕栽培バッファー剤植物水分計測植物工場イオン透過性ゼオライト

项目摘要

Although hydroponic culture has been used more in recent years as a cultivation method, there are problems for caltivating crops for commercially high quality products. In higher concentrations of nutrient solutions, physiological disorders can be easily induced in crops grown under hydroponic cultivation conditions although soil culture does not cause damages in crops.In the present study, new culture mediums for hydroponic culture and tissue culture were developed by using colloidal buffer agents. When the concentration of nutrient solution was incresed in hydroponic culture for tomato cultivation, blossom-end rot could be induced significantly although more sugars accumulated in fruits. When zeolite was added to the hydroponic solution, the occurrence of the blossom-end rot was prevented significantly.A new tissue culture medium was developed by mixing colloidal buffer agents together with tissue culture medium solution. Powers of zeolite, bentonite and charcoal were added to the medium solution as colloidal buffer agents. By adding colloidal buffer agents, the buffering capacity of pH in the medium increased significantly against both acidification and alkalization. When embryos were grown in mediums containing colloidal buffer agents, the length of plantlets formed from the embryos became larger and the dry weight of the plantlets became heavier than plantlets grown in mediums containing no buffering agents.These findings suggest that colloidal buffer agents stabilize the pH of the culture medium and sustain cell elongation and growth of plantlets for growing period during tissue culture.Furthermore, the addition of colloidal buffers in culture mediums had an additional benefit of preventing the degradation of organic substances in the culture media while the media were heated for the purpose of sterilization.

尽管近年来水培培养物已被用作一种培养方法，但仍有为商业高质量产品造成农作物的问题。在较高浓度的养分溶液中，尽管土壤培养不会在农作物中造成损害，但在水文培养条件下生长的农作物中很容易诱导生理疾病。在本研究中，通过使用胶体缓冲剂使用胶质缓冲剂，开发了用于水培培养和组织培养的新的培养基。当在水培培养物中增加养分溶液的浓度以进行番茄培养时，尽管在水果中积累了更多的糖，但可以显着诱导开花端腐烂。当将沸石添加到水培溶液中时，明显预防开花末端腐烂的发生。通过将胶体缓冲剂与组织培养基培养基混合在一起，开发了一种新的组织培养基。将沸石，膨润土和木炭的功率添加到中溶液中，作为胶体缓冲剂。通过添加胶体缓冲剂，培养基中pH的缓冲能力与酸化和碱化化显着增加。当在含有胶体缓冲剂剂的培养基中生长胚胎时，由胚胎形成的植物长度变大，植物群的干重比在没有缓冲剂的培养基中生长的植物会更重。这些发现表明，这些发现表明胶体缓冲剂在培养基和维持培养基的pH中稳定植物的生长，使培养基的pH稳定在培养基中成长。培养基中的胶体缓冲液具有额外的好处，可以防止培养基在培养基中加热培养基中有机物质的降解。