PRECISE QUANTIZATION OF PHASE CONTRAST MICROSCOPE FOR OBSERVATION OF A BIOLOGICAL SAMPLE USING LIQUID CRYSTAL PHASE MODULATOR

使用液晶相位调制器对生物样品观察用相衬显微镜进行精确量化

• 批准号: 04555011
• 负责人: TOYOOKA Satoru
• 金额: $ 3.52万
• 依托单位: SAITAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04555011/
• 关键词: Phase contrast microscope; Phase modulation device; Optical nonlinearity; Liquid crystals; Green tea extra; Phase shift method; Deconvolution; 強誘電性液晶; 反強誘電性液晶; 液晶位相変調素子; 生物細胞; ネマティック液晶

Phase contrast microscope (PCM) is a key device to observe biological samples. But conventional PCM is not suited for quantitative observation. We have had an idea to quantize phase contrast image by using a variable phase shifter such as liquid crystal devices instead of a fixed phase plate. To realize the idea, following investigations have been done.1) A common path interferometer with nematic liquid crystal (LC) phase shifter was equipped to quantize phase distribution of a 2-D phase object. Accuracy of 30/lambda was attained.2) To observe live biological samples, high speed data access is required. Surface stabilized ferro-electric LC and antiferro-electric LC were investigated as high speed phase shifters. We found that these devices prepare required characteristics for phase shifters in PCM.3) Nonlinear optical properties of green tea extra and resultant phase modulation properties were investigated. Full range phase modulation from 0 to 2pi is possible by changing absorbed power. Applications of this phenomena as a self-align phase filter were demonstrated.4) A PCM made on experimental basis having an equivalent pupil plane out of the optical tube separated from the objective lens was equiped. The LC phase shifter having a ring-shape electrode corresponding to the ring slit is placed on the pupil plane. Phase quantization method based on deconvolution of a subtracted image between +pi/2 and -pi/2 phase shift images was proposed and analyzed results were demonstrated.

相差显微镜（PCM）是观察生物样品的关键设备。但传统的PCM不适合于定量观测。我们提出了用可变移相器（如液晶器件）代替固定相位板来再现相衬像的想法。为了实现这一思想，我们进行了以下研究：1）采用共光路干涉仪和液晶相移器，测量了二维位相物体的位相分布。达到30/lambda的精度。2）为了观察活体生物样品，需要高速数据访问。研究了表面稳定的铁电和反铁电液晶作为高速移相器。研究了绿色茶的非线性光学特性及其相位调制特性。通过改变吸收功率，可以实现从0到2 pi的全范围相位调制。4）在实验的基础上研制了一种在光管外有等效光瞳面的PCM，该等效光瞳面与物镜透镜分离。具有与环形缝隙相对应的环形电极的LC移相器放置在光瞳平面上。提出了一种基于+pi/2和-pi/2相移图像之间的减影图像去卷积的相位量化方法，并给出了分析结果。

项目成果

豊岡了 他: "強誘電性液晶の位相変調特性(2)" 第40回応用物理学関係連合講演会予稿集III. 897 (1993)

Ryo Toyooka等：“铁电液晶的相位调制特性(2)”第40届应用物理联席会议论文集III 897(1993)。

H.Kadono and S.Toyooka: "High precision common path interferometer using a liquid crystal phase modulator" Proc.Int.Conf.from Galileo's "Ochhialino" to optelectronics. 895-900

H.Kadono 和 S.Toyooka：“使用液晶相位调制器的高精度共路干涉仪”Proc.Int.Conf.，从伽利略的“Ochhialino”到光电子学。

豊岡了 他: "液晶位相変調器とその干渉計測への応用" 理研シンポジウム「新しい光応用技術IX」講演予稿集. 5-8 (1993)

Ryo Toyooka 等人：“液晶相位调制器及其在干涉测量中的应用”RIKEN 研讨会“新光学应用技术 IX”论文集 5-8 (1993)。

H.Kadono, R.Uma Maheswari, R.Silvenoinen, and T.Jaaskelainen: "Self-align phase filter using optical properties of chinese tea" Extended Abstracts of JSAP. 40. 881 (1993)

H.Kadono、R.Uma Maheswari、R.Silvenoinen 和 T.Jaaskelainen：“利用中国茶光学特性的自对准相位滤波器”JSAP 扩展摘要。

豊岡了 他: "強誘電性液晶の位相変調特性(3)" 第54回応用物理学会講演予稿集III. (1994)

Ryo Toyooka 等：“铁电液晶的相位调制特性（3）”第 54 届日本应用物理学会会议论文集 III（1994 年）。