权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of the bioreactor to produce poly-D-3-hydroxybutyric acid from carbon dioxide

开发利用二氧化碳生产聚-D-3-羟基丁酸的生物反应器

基本信息

批准号：
04556011
负责人：
ISIZAKI Ayaaki
金额：
$ 12.29万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04556011/
关键词：
crbon dioxide poly-D-3-hydroxybutyric acid PHB biodegradable plastic bioreactor hydrogen bacteria two stage culture autotroph バイオリアクタ-

项目摘要

The bioprocess to produce poly-D-3-hydroxybutyric acid from carbon dioxide has been investigated by the collaboration with the research laboratory of Saibu Gas Co.Ltd., (Fukuoka) to tackle the two global environmental tasks, green house effect and pollution with plastics. Hydrogen bacteria Alcaligenes eutrophus which is autotrophically cultured under oxygen limitation produces poly-D-3-hydroxybutyric acid, biodegradable polyester, which can be processed into new plastics. To success this project, however, needs the high level safety system which can absolutely avoid gas explosion and the bioreactor which can provide the high gas mass transfer capacity. The condition of the substrate gas supply to guarantee process safety, growth, and PHB production was determined by the empirical stoichiometry derived from the autotrophic culture of this microorganism. The very high level explosion-proof bench plant in which the jar fermentor of K_La 2790 h^<-1> was used was installed in the research laboratory of Saibu Gas Co.Ltd. This bioprocess attained 62 g/1 of PHB production at 91 g/1h of dried cell weight (DCW) under the explosion free substrate gas composition (oxygen below 6.9%). However, many bottlenecks were still unsolved for the industrial application of this bioprocess. We, therefore, developed the two stage culture process wich is consisting of the heterotroph culture stage for cell growth and the autotroph culture stage for PHB production. Thus, this process gave the satisfactory PHB productivity of 0.6 g/1h by the jar fermentor of K_La 340 h^<-1>. The pilot plant in which the air lift type bioreactor was used was installed in the research laboratory of Saibu Gas Co.Ltd.

研究了以二氧化碳为原料生产聚d -3-羟基丁酸的生物工艺。（福冈）致力于解决温室效应和塑料污染这两项全球环境任务。富营养化氢细菌Alcaligenes在限氧条件下自养培养，产生聚d -3-羟基丁酸，可降解聚酯，可加工成新型塑料。然而，该项目的成功需要高水平的安全系统，绝对避免气体爆炸和生物反应器，可以提供高的气体传质能力。通过该微生物自养培养的经验化学计量学确定了保证工艺安全、生长和PHB生产的基质供气条件。采用K_La 2790 h^<-1>罐式发烧罐的超高级防爆台架装置安装在赛步燃气有限公司研究实验室。在无爆炸底物气体组成（氧含量低于6.9%）下，该生物工艺在干燥细胞重量（DCW）为91 g/1h的条件下，PHB的产量达到62 g/1。然而，这种生物工艺的工业应用仍有许多瓶颈尚未解决。因此，我们开发了两阶段的培养过程，即细胞生长的异养培养阶段和产生PHB的自养培养阶段。结果表明，该工艺在K_La 340 h^<-1>的罐式发酵罐上获得了令人满意的PHB产率0.6 g/1h。气升式生物反应器的中试装置安装在赛步燃气股份有限公司的研究实验室。