Development and Application of Reconstruction System for Morphological Study by Using Workstation

我们一直在为形态学研究者开发一个三维重建系统。在开发过程中，我们阐明了各种问题，我们正在逐步解决这些问题。计算机重建是一个耗时的过程，包括数字数据的传输、处理和管理。这给了我们艰苦的工作。为了改善这些问题，我们开发了一个综合系统。利用工作站的优势，机器通过局域网相互连接，局域网进一步扩展到互联网（由日本教育、科学和文化部信息中心管理的SINET）。这使我们能够与世界上许多研究人员进行广泛的合作。在网络计算与形态学研究者重构系统的整合过程中，我们注意到了形而上学的问题。为了寻求解决方案，我们发布了我们的经验，想法和提示。由于彩色图像数据量巨大，我们将其作为模拟数据存储到激光视频记录器（LVR）中，在工作站控制的帮助下检索它们，并将它们作为数据库，通过模拟/数字转换在工作站上获得数字数据。利用工作站控制显微镜载物台和LVR，将组织切片的大面积全彩色图像自动分割成多幅图像，存储在LVR中，并进行缩小处理，以蒙太奇图像的形式在工作站显示器上重新显示。显示器上感兴趣结构的轮廓通过样条函数将代表点互连而获得，并且通过指定轮廓的内部部分而产生三维mataball数据。通过使用RPC（远程过程调用）将耗时的进程分发到高性能的CPU服务器。

We have been developing a three-dimensional reconstruction system for morphological researchers. In the development process, we elucidated various issues, which we are resolving step by step. The computer reconstruction is a time consuming process including the transportation, handing and management of digital data. This gives us laborious works. To improve them, we have developed an integrated system. Taking advantages of workstations, machines are connected with each other via a local area network, which is further extended to the Internet (SINET run by the Information Center of the Ministry of Education, Science and Culture in Japan). THis enabled us to cooperate with many researchers in wide area on the world. In the process of the integration of the Internet computing and the reconstruction system for the morphological researchers, we noticed metaphysical issues. In search of the resolution, we published our experiences, idea and tips. Due to the immense color image data, we stored them as analog data into a laser video recoder (LVR), retrieved them with the help of workstation control, and made them as a database to get digital data on workstations via analog/digital conversion. By controlling the stage of microscope and the LVR with the workstation, the wide area full color image of histological section are automatically splited into many pieces, which are stored into the LVR,miniaturized and redisplayd as a montage image on the workstation display. The outlines of structures of interest on the display are obtained by interconnecting representative points via spline function, and three-dimensional mataball data are produced by designating the inner portion of the outline. The time-consuming processes are distributed to high performance CPU servers by using RPC (Remote Procedure call).