Establishment of prenatal diagnosis of epidermolysis bullosa in Japan

日本大疱性表皮松解症产前诊断的建立

基本信息

  • 批准号:
    04557045
  • 负责人:
  • 金额:
    $ 5.57万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

With the Grant-in-Aid for Scientific Research (B), we have made a prenatal diagnosis of epidermolysis bullosa for the first time in Japan. Prenatal diagnosis of epidermolysis bullosa using fetal skin biopsy specimens has been successfully performed in Europe and America, but this technique has not previously been attempted in Asia. We, for the first time in Asia, attempted tomake a prenatal diagnosis of epidermolysis bullosa in high-risk fetus by fetal skin biopsy. A skin biopsy was obtained from the fetus as risk of gravis type ofjunctional epidermolysis bullosa of Herlitz. The biopsy specime was studied by electron microscopy and by immunohistochemistry using various monoclonal antibodies against epidermal basement membrane zone. There were were no ultrastructural abnormalities in the basement membrane zone, including the hemidesmosomes. Indirect immunofluorescence study showed normal expression of GB3 antigen. The pregnancy was continued, and a normal, healthy infant was born. The prenatal diagnosis of epidermolysis bullosa is now available in Tokyo. This clinical diagnostic service is available to families from various parts of Asia.
通过科学研究补助金(B),我们在日本首次进行了大疱性表皮松解症的产前诊断。使用胎儿皮肤活检标本进行大疱性表皮松解症的产前诊断已在欧洲和美国成功进行,但该技术此前在亚洲尚未尝试过。我们在亚洲首次尝试通过胎儿皮肤活检对高危胎儿进行大疱性表皮松解症产前诊断。对胎儿进行了皮肤活检,发现其有患重症型赫利茨交界性大疱性表皮松解症的风险。通过电子显微镜和免疫组织化学使用针对表皮基底膜区域的各种单克隆抗体对活检标本进行研究。基底膜区(包括半桥粒)没有超微结构异常。间接免疫荧光研究显示GB3抗原表达正常。妊娠继续进行,并出生了一个正常、健康的婴儿。东京现已提供大疱性表皮松解症的产前诊断。这项临床诊断服务可供来自亚洲各地的家庭使用。

项目成果

期刊论文数量(39)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Akiyama,M.: "Ultrastructural localization of Brazilian pemphigus foliaceus(fogo selvagem)antigens in cultured human squamous cell carcinoma cells." Br J Dermatol. 128. 378-383 (1993)
Akiyama,M.:“培养的人鳞状细胞癌细胞中巴西落叶型天疱疮(fogo selvagem)抗原的超微结构定位。”
  • DOI:
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    0
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Shimizu, H., Ishida-Yamamoto, A., Eady, R.A.J.: "The use of silver-enhanced 1-nm gold probes for light and electron microscopic localization of intra- and extracellular antigens in skin." J Histochem Cytochem. 40(6). 883-888 (1992)
Shimizu, H.、Ishida-Yamamoto, A.、Eady, R.A.J.:“使用银增强 1 nm 金探针进行皮肤细胞内和细胞外抗原的光和电子显微镜定位。”
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    0
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西川 武二: "皮膚の疾患 最新の治療" 南江堂, 2 (1993)
西川武二:“皮肤病的最新治疗方法” Nankodo,2 (1993)
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    0
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Ebihara, T., Hashimoto, T., Kudoh, J., Gamou, S., Shimizu, N., Nishikawa, T.: "Detection of the 170-kDa bullous pemphigoid antigen by immunoprecipitation." J Invest Dermatol. 100(2). 176-179 (1993)
Ebihara, T.、Hashimoto, T.、Kudoh, J.、Gamou, S.、Shimizu, N.、Nishikawa, T.:“通过免疫沉淀法检测 170 kDa 大疱性类天疱疮抗原。”
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    0
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Shimizu, H., Onodera, Y., Ikeda, S., Ogawa, H., Suzumori, K., Nishikawa, T.: "Prenatal diagnosis of epidermolysis bullosa : First successful trial in Asia." Dermatology. 188(1). 46-49 (1994)
Shimizu, H.、Onodera, Y.、Ikeda, S.、Okawa, H.、Suzumori, K.、Nishikawa, T.:“大疱性表皮松解症的产前诊断:亚洲首次成功试验。”
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NISHIKAWA Takeji其他文献

NISHIKAWA Takeji的其他文献

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{{ truncateString('NISHIKAWA Takeji', 18)}}的其他基金

Real-time imaging analysis of cell adhesion molecules of epidermal keratinocytes
表皮角质形成细胞细胞粘附分子的实时成像分析
  • 批准号:
    14370262
  • 财政年份:
    2002
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Basic studies for development of disease-specific therapeutic strategies against autoimmune diseases
开发针对自身免疫性疾病的疾病特异性治疗策略的基础研究
  • 批准号:
    12470181
  • 财政年份:
    2000
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Evaluation of immune suppressive therapy using autoimmune model mouse
使用自身免疫模型小鼠评价免疫抑制治疗
  • 批准号:
    12557072
  • 财政年份:
    2000
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of novel mouse model for autoimmune diseases using autoantigen knockout mice
使用自身抗原敲除小鼠开发新型自身免疫性疾病小鼠模型
  • 批准号:
    10470189
  • 财政年份:
    1998
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Elucidation of Pathogenetic Mechanisms of Severe Ichthyoses and Establishment of New Method for the Diagnosis and Prenatal Disease Detection
阐明重度鱼鳞病发病机制及建立诊断及产前疾病检测新方法
  • 批准号:
    10557082
  • 财政年份:
    1998
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of novel therapy against bullous diseases
针对大疱性疾病的新疗法的开发
  • 批准号:
    10044318
  • 财政年份:
    1998
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Development of a novel diagnostic tool using recombinant pemphigus antigens with the proper native conformation.
使用具有正确天然构象的重组天疱疮抗原开发新型诊断工具。
  • 批准号:
    07557064
  • 财政年份:
    1995
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Clarification of pathogenesis in pemphigus : Establishment of new techniques for diagnosis.
阐明天疱疮发病机制:建立诊断新技术。
  • 批准号:
    05404036
  • 财政年份:
    1993
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Basic studies for the pathogenesis and diagnosis of the autoimmune bullous diseases
自身免疫性大疱性疾病发病机制及诊断的基础研究
  • 批准号:
    05044186
  • 财政年份:
    1993
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Application of up-to date immunoelectron microscopy for the study of pathogenesis of skin blistering disease.
应用最新免疫电子显微镜研究皮肤水疱病的发病机制。
  • 批准号:
    03454275
  • 财政年份:
    1991
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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Study of the Safety, Tolerability, and Efficacy of an iPS Cell-based Therapy for Recessive Dystrophic Epidermolysis Bullosa Delivered with a Spray on Skin Device
使用皮肤喷雾装置治疗隐性营养不良性大疱性表皮松解症的 iPS 细胞疗法的安全性、耐受性和有效性研究
  • 批准号:
    10721324
  • 财政年份:
    2023
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    $ 5.57万
  • 项目类别:
Identifying and overcoming factors contributing to delayed wound healing in epidermolysis bullosa.
识别并克服导致大疱性表皮松解症伤口愈合延迟的因素。
  • 批准号:
    23H02928
  • 财政年份:
    2023
  • 资助金额:
    $ 5.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Epigenetic predisposition and pathogenic mechanisms of recessive dystrophic epidermolysis bullosa
隐性营养不良性大疱性表皮松解症的表观遗传倾向和致病机制
  • 批准号:
    10673176
  • 财政年份:
    2022
  • 资助金额:
    $ 5.57万
  • 项目类别:
Epigenetic predisposition and pathogenic mechanisms of recessive dystrophic epidermolysis bullosa
隐性营养不良性大疱性表皮松解症的表观遗传倾向和致病机制
  • 批准号:
    10453091
  • 财政年份:
    2022
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    $ 5.57万
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Cutaneous neoplasias in epidermolysis bullosa: molecular characterization and application of augmented intelligence
大疱性表皮松解症中的皮肤肿瘤:分子特征和增强智能的应用
  • 批准号:
    459731775
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    2021
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    $ 5.57万
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    WBP Fellowship
A Pivotal Phase 3 Study of FCX-007 (Genetically-Modified Autologous Human Dermal Fibroblasts) for Recessive Dystrophic Epidermolysis Bullosa
FCX-007(转基因自体真皮成纤维细胞)治疗隐性营养不良性大疱性表皮松解症的关键 3 期研究
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    10544379
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    2021
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    $ 5.57万
  • 项目类别:
Therapeutic Application of CRISPR-Cas3 for dominant dystrophic Epidermolysis Bullosa
CRISPR-Cas3在显性营养不良性大疱性表皮松解症中的治疗应用
  • 批准号:
    21K16230
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    2021
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    $ 5.57万
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Study for developmental origin of mesenchymal blood cells and their regenerative activity in epidermolysis bullosa
大疱性表皮松解症间充质血细胞的发育起源及其再生活性研究
  • 批准号:
    19H03682
  • 财政年份:
    2019
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    $ 5.57万
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探索隐性营养不良性大疱性表皮松解症的替代 iPS 细胞疗法
  • 批准号:
    9811321
  • 财政年份:
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Homeostasis of the epidermal cells and its dysfunction in epidermolysis bullosa
大疱性表皮松解症中表皮细胞的稳态及其功能障碍
  • 批准号:
    19H03449
  • 财政年份:
    2019
  • 资助金额:
    $ 5.57万
  • 项目类别:
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