Fibroblast growth factor in saliva and oral lesions

唾液和口腔病变中的成纤维细胞生长因子

• 批准号: 04557077
• 负责人: SAKU Takashi
• 金额: $ 5.63万
• 依托单位: Niigata University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557077/
• 关键词: fibroblast growth factor (FGF); acidic FGF; basis FGF; immunochemistry; saliva; salivary gland; oral wound healing; epulides; 線維芽細胞成長因子; 唾液線; ELISA; in-site ハイブリダイゼーション; ウェスタンブロット; 歯周疾患; マイクロファージ; in-situハイブリダイゼーション; マクロファージ; ドットブロット

We have carried out the following experiments and determined the immunolocalization acidic/basic fibroblast growth factors (aFGF/bFGF) in various pathophysiologic conditions and the salivary FGF levels.Salivary FGF level ; Using ELISA method, we determined bFGF in the human saliva and the bovine submandibular gland tissue. We could realize the detection sensitivity of 0.1 ng/ml of bFGF by the sandwith ELISA using two kinds of monoclonal antibodies. bFGF was enriched in salivary gland tissues at concentration of 1.1 ng/ml, that was 5 to 6 times of the normal human serum level. However, bFGF was not detected in the saliva with this system.In order to determine the role of bFGF in gingival wound healing, we examined surgical materials of epulides containing various stages of granulation tissue, using the immunoperoxidase and immunofluorescence and formalin fixed paraffin sections. bFGF and heparan sulfate proteoglycan were co-immunolocalized in newly synthesized stroma in the middle organizing stage of granulation tissue. bFGF was also immunolocalized in macrophages, mast cells and vascular endothelial cells. bFGF-positive macrophages mostly increased in number at the middle stage. These findings suggest that bFGF is released from some of macrophages and mast cells into the extracellular milieu when granulation tissue is active in neovascularization but not yet for fibrosis.Application of FGF determination to the clinical examination has not yet developed. However, we need more fundamental preparation before it is really applied to the saliva test.

我们进行了以下实验，测定了不同病理生理条件下免疫定位酸性/碱性成纤维细胞生长因子（aFGF/bFGF）和唾液中FGF的水平。唾液FGF水平；采用ELISA法测定人唾液和牛颌下腺组织中bFGF的含量。两种单克隆抗体均可实现ELISA检测bFGF 0.1 ng/ml的灵敏度。bFGF在唾液腺组织中富集，浓度为1.1 ng/ml，是正常人血清水平的5 ~ 6倍。然而，该系统未在唾液中检测到bFGF。为了确定bFGF在牙龈伤口愈合中的作用，我们使用免疫过氧化物酶、免疫荧光和福尔马林固定石蜡切片检查了含有不同阶段肉芽组织的手术材料。在肉芽组织形成中期，bFGF和硫酸肝素蛋白多糖在新合成的基质中共同免疫定位。bFGF在巨噬细胞、肥大细胞和血管内皮细胞中也有免疫定位。bfgf阳性巨噬细胞多数在中期增多。这些发现表明，当肉芽组织在新生血管形成中活跃时，bFGF从一些巨噬细胞和肥大细胞释放到细胞外环境中，但尚未用于纤维化。FGF测定在临床检查中的应用尚未发展。然而，在真正应用于唾液测试之前，我们还需要更多的基础准备。

项目成果

Murata M,Hara K,Yamamoto K,Saku T.: "Immunohistochemical localization of basic fibroblast growth factor in gingival granulation tissue" Jpn J Oral Biol.36(S). 195 (1994)

Murata M，Hara K，Yamamoto K，Saku T.：“牙龈肉芽组织中碱性成纤维细胞生长因子的免疫组织化学定位”Jpn J Oral Biol.36（S）。

Saku T,Rintoku S,Tsuda A,Sasaki H,Koiwai k.: "Immunohistochemical localization of heparan suafate proteoglycan and fibroblast growth factor in the fibroadenoma of mammary gland" Tr Soc Pathol Jpn.80(1). 155 (1991)

Saku T、Rintoku S、Tsuda A、Sasaki H、Koiwai k.：“乳腺纤维腺瘤中乙酰肝素硫磺酸蛋白聚糖和成纤维细胞生长因子的免疫组织化学定位”Tr Soc Pathol Jpn.80(1)。

Saku T,Sasaki H.: "Immunohistochemistry of basic fibroblast growth factor binding and its application for oral mucosal legion" Tr Soc Pathol Jpn.81(1). 114 (1992)

Saku T，Sasaki H.：“碱性成纤维细胞生长因子结合的免疫组织化学及其在口腔粘膜军团中的应用”Tr Soc Pathol Jpn.81（1）。

Murata M,Hara K,Saku T.: "Immunohistochemical localization of bFGF in gingival wound healing" J Dent Res.74(S). 508 (1995)

Murata M、Hara K、Saku T.：“bFGF 在牙龈伤口愈合中的免疫组织化学定位”J Dent Res.74(S)。

程,〓 他: "唾液腺腺様嚢胞癌における線維芽細胞増殖因子の免疫組織化学的局在" 歯科基礎医学会雑誌. 33. 254 (1991)

Cheng 等：“唾液腺腺样囊性癌中成纤维细胞生长因子的免疫组织化学定位”日本基础牙科医学会杂志 33. 254 (1991)。