Establishment of immotalized cell lines from transgenic mouse nephron segments

从转基因小鼠肾单位片段建立永生化细胞系

基本信息

项目摘要

The results that we could obtain according to our plan are as follows.1)Establishment of immortalized cultured cell lines from transgenic mouse kidneys.A ts-SV40 large T-antigen gene was injected into pronuclei of fertilized eggs of C57BL/6 mice, and mice of first generation were abtained. Various nephron segments were microdissected from collagenase-treated kidneys and cultivated. Cells thus made can be maintained over 100 passages.2)Characterization of established cell lines.Several characters like nephron segment-specific enzymes, metabolisms, and hormone recepters in the cell lines were compared with those in freshly isolated respective nephron segments. These cell lines maintained most site-specific characters except gamma-GTP activity, gluconeogenesis and porathyroid hormone receptor.3)Application of these cell lines for pharmacological and toxicological studies.Cells originated from the terminal proximal tubule could be successfully applied for the evaluation of cisplatin-induced nephrotoxicity. Since ATP-purinoceptor was highly expressed in cells form outer medullary collecting duct, these cells might be suitable sample for cloning cDNA of ATP receptr protein (s) . This trial is now under investigation.
1)建立转基因小鼠肾脏永生化培养细胞系将ts-SV 40大T抗原基因注入C57 BL/6小鼠受精卵原核,获得第一代小鼠。从胶原酶处理的肾脏显微切割各种肾单位片段并培养。2)建立的细胞系的特性:将该细胞系中的肾单位节段特异性酶、代谢和激素受体等特性与新鲜分离的相应肾单位节段进行比较。这些细胞系除γ-GTP活性、促性腺激素生成和甲状腺激素受体外,其余均保持了大部分位点特异性特征。3)这些细胞系在药理学和毒理学研究中的应用:来源于终末近曲小管的细胞可成功用于评价顺铂的肾毒性。由于ATP-嘌呤受体在外髓集合管细胞中高表达,这些细胞可能是克隆ATP受体蛋白cDNA的合适样本。此案正在调查中。

项目成果

期刊论文数量(43)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takeda,M.: "Intranephron distribution of creatine content in rats" Biochemistry and Molecular Biology International. 32. 435-440 (1994)
Takeda,M.:“大鼠肌酸含量的肾单位分布”国际生物化学和分子生物学。
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Takeda, M. , Hosoyamada, M. , Shirato, I. , Obinata, M. , Suzuki, M. and Endou, H.: "Establishment of vasopressin-responsive early proximal tubular cell lines derived from transgenic mice harboing temperature-sensitive simian virus 40 large T-antigen gene
Takeda, M.、Hosoyamada, M.、Shirato, I.、Obinata, M.、Suzuki, M. 和 Endou, H.:“建立源自温度敏感猿猴的转基因小鼠的加压素响应性早期近端肾小管细胞系
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遠藤 仁(丸茂文昭、黒川清、越川昭三、藪田敬次郎、小川龍、北島政樹 編集): "輸液ハンドブック" 中外医学社, 479 (1994)
远藤仁(丸茂文明、黑川清、越川正三、薮田敬二郎、小川龙、北岛正树编):《输液手册》中外医学社,479(1994)
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Kanai, Y,and Endou, H: "Roles of glutamate transporters in neuronal cell death" J.Toxicol.Sci. 19. 295-297 (1994)
Kanai, Y 和 Endou, H:“谷氨酸转运蛋白在神经元细胞死亡中的作用”J.Toxicol.Sci。
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Takeda, M., Hosoyamada, M., Shirato, I., Obinata, M., Suzuki, M.and Endou, H: "Establishment of vasopressin-responsive early proximal tubular cell lines derived from transgenic mice harboing temperature-sensitive simian virus 40large T-antigen gene." Bioc
Takeda, M.、Hosoyamada, M.、Shirato, I.、Obinata, M.、Suzuki, M.和 Endou, H:“建立源自携带温度敏感猿病毒的转基因小鼠的加压素反应性早期近端肾小管细胞系
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ENDOU Hitoshi其他文献

ENDOU Hitoshi的其他文献

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{{ truncateString('ENDOU Hitoshi', 18)}}的其他基金

Development of novel anti-uricosuric agents based on the genomic strategy.
基于基因组策略开发新型抗尿酸排泄药物。
  • 批准号:
    14207004
  • 财政年份:
    2002
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Genetic Abnormality of Renal Proximal Tubule-Specific Transporters as Causes of Sudden Death Syndrome in South-Eastern Asia
肾近端小管特异性转运蛋白的遗传异常是东南亚猝死综合症的原因
  • 批准号:
    13376004
  • 财政年份:
    2001
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Identification of transporter genes regulating systemic kinetics of drugs and foreign compounds and their genetic polymorphism
调节药物和外来化合物全身动力学的转运蛋白基因的鉴定及其遗传多态性
  • 批准号:
    12357016
  • 财政年份:
    2000
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular mechanisms of drug transport across cell membrane
药物跨细胞膜转运的分子机制
  • 批准号:
    11694310
  • 财政年份:
    1999
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular cloning and functional expression of kidney-specific organic anionic drug transporters
肾脏特异性有机阴离子药物转运蛋白的分子克隆及功能表达
  • 批准号:
    09470025
  • 财政年份:
    1997
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Role of vanadium in endemic diseases in Northeast Thailand
钒在泰国东北部地方病中的作用
  • 批准号:
    07041167
  • 财政年份:
    1995
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Endemic Primary Distal Renal Tubular Acidosis in Thailand
泰国地方性原发性远端肾小管酸中毒
  • 批准号:
    04041041
  • 财政年份:
    1992
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Establishment of screening systems for evaluating drug actions in the kidney
建立评估肾脏药物作用的筛选系统
  • 批准号:
    01870111
  • 财政年份:
    1989
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
ULTRAMICRO METHODS FOR DETERMINING ENZYME ACTIVITIES AND SUBSTRATES USING COMBINED BIOLUMINESCENT ASSAY WITH ENZYMATIC CYCLING
使用生物发光测定与酶循环相结合测定酶活性和底物的超微方法
  • 批准号:
    62870009
  • 财政年份:
    1987
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Intrarenal actions of atrial natriuretic peptides
心房钠尿肽的肾内作用
  • 批准号:
    61570094
  • 财政年份:
    1986
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Development of inner blood-retinal barrier cell lines from transgehic rats harboring temperature-sensitive SV40 large T-antigen gene
从携带温度敏感的SV40大T抗原基因的转基因大鼠中开发内血视网膜屏障细胞系
  • 批准号:
    12557226
  • 财政年份:
    2000
  • 资助金额:
    $ 11.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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