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STUDIES ON NEW TREATMENT METHOD FOR LIPIDCONTAINIG WASTEWATER USING BACTERIA

含脂废水细菌处理新方法的研究

基本信息

批准号：
05556012
负责人：
IZAKI Kazuo
金额：
$ 8.58万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05556012/
关键词：
Theatment of lipid-containing wastewater Lipase-producing bacteria Pseudomonas fragi A water circulation system Lipase-requlating protein Gene engineering of bacteria Cloning of lipase gene Shuttle vector リパーゼB遺伝子のクローン化リパーゼB構造遺伝子リパーゼ

项目摘要

1.Taxonomic studies on No.6 strain, which is effective for treatment of lipid-containing wastewater were carried out and No.6 strain was considered to belong to Genus Pseudomonas from the following results ; rod, gram-negative, polar flagella, motile, no spore, oxidase-negative and acid formed without gas from glucose.2.Effects of temperature and concentration of NaCl on growth of two lipid-assimilating strains of bacteria, Bacillus No.351 and Pseudomonas No.6 were studied. No.6 strain was resistant to high temperature and high concentration of NaCl compared to No.351 strain. Effective removal of lipid from lipid-containing wastewater could be done even in the presence of NaCl and under high temperatue using No.6 strain.3.New lipid-assimilating bacteria were isolated from various sources in Hachinohe city. One strain had a quite high lipase activity at 37ﾟC and the other strain had stronger activity at 20ﾟC than 37ﾟC.Further work should be done for treatment of lipid-containing wastewater using the new isolated strains.4.In order to improve lipid-assimilating activity of Pseudomonas, we cloned a lipase B gene of Pseudomonas fragi 22-39B strain and derermined the nucleotide sequences of the lipase B gene. We discovered a new open reading frame (ORF1) which is located down stream of the structural gene of the lipase B.We found that both the structural gene of the lipase and the ORF1 are required for the expression of the lipase B activity. Further work should be done for clarifying the role of the ORF1 on the lipase activity and breeding of a new strain which has both strong lipase and lipid-assilmilating activities using gene engineering techniques. The new strain will be useful for the treatment of lipid-containing wastewater.

1.对处理含油脂废水的6号菌株进行了分类学研究，结果表明6号菌株属于假单胞菌属; 2.温度和NaCl浓度对两株脂肪同化细菌生长的影响，对芽孢杆菌351号和假单胞菌6号进行了研究。6号菌株比351号菌株耐高温、耐高浓度NaCl。6号菌株在高温和NaCl条件下也能有效去除含油脂废水中的油脂。3.从八户市不同来源分离到一株新的油脂同化菌。为了提高假单胞菌的油脂同化活性，克隆了Fragi假单胞菌22- 39 B菌株的脂肪酶B基因，并对脂肪酶B基因进行了核苷酸序列测定。我们发现了一个新的开放阅读框（ORF 1），它位于脂肪酶B结构基因的下游，发现脂肪酶B活性的表达需要脂肪酶结构基因和ORF 1。进一步研究ORF 1在脂肪酶活性中的作用以及利用基因工程技术选育兼具脂肪酶和类脂酶活性的新菌株是今后的工作重点。该菌株可用于油脂废水的处理。