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THE STUDY OF RAPID DIAGNOSIS TECHNIQUE OF VIRUS DISEASES IN VEGETATIVELY PROPAGATIVE CROPS

无性繁殖作物病毒病快速诊断技术的研究

基本信息

批准号：
05556007
负责人：
KAMEYA(IWAKI) Mitsuro
金额：
$ 4.93万
依托单位：
FACULTY OF AGRICULTURE,YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05556007/
关键词：
Vegetatively propagative crops Plant viruses Rapid diagnosis Serology Latex Filter paper

项目摘要

Rapid immunofilter paper assay (RIPA) was applied for the diagnosis of the virus occurring in vegetatively propagative crops. RIPA was developed using latex beads coated with antibody to virus. White latex-coated antibody was immobilized as a solid in a line on the surface near the bottom of a filter paper strip (8x0.5cm). The bottom end of this paper strip was dipped for 1 min into a leaf extract from infected plant and then for 2 min into dyed latex coated with the virus antibody. A colored band appeared on the line where the white latex had been immobilized. RIPA is suitanlble for field diagnosis because that does not requie specialized equipment and is time-saving.Konjak mosaic virus, dasheen mosaic virus and cucumber mosaic virus in konjak, chrysanthemum virus B in chrysanthemum, potato leaf roll virus, potato virus X and potato virus Y in potato, odontoglossum ringspot virus and cymbidium mosaic virus in orchids, garlic latent virus and onion yellow dwaft virus in welsh onion and strawberry pseudo mild yellow edge virus in strawberry were detected by RIPA.Simple method of RIPA was developed by plotting antibody-coated white and dyed latexes on filter strip before testing. Another modification of RIPA to flow horizontally leaf extract and antibody-coated dyed latex was developed to detect of viruses from plants infected with multiple viruses.

应用快速免疫滤纸试验(RIPA)对发生在无性繁殖作物上的病毒进行了诊断。RIPA是用涂有病毒抗体的乳胶珠开发的。白色乳胶包被抗体以固体形式固定在滤纸条底部附近的表面(8×0.5 cm)。将试纸底端浸泡于病株叶片提取液中1min，再浸入涂有病毒抗体的染色乳胶中2min。白色乳胶固定的那条线上出现了一条彩色带子。魔芋上的魔芋花叶病毒、黄瓜花叶病毒和黄瓜花叶病毒，菊花上的菊花病毒B，马铃薯上的马铃薯卷叶病毒，马铃薯上的马铃薯X病毒和马铃薯Y病毒，兰花上的兰花环斑病毒和兰花叶病毒，大葱上的大蒜潜伏病毒和洋葱黄花叶病毒，草莓上的大蒜潜伏病毒和洋葱黄边病毒。RIPA的另一个改进是水平流动的叶提取物和抗体包被的染色胶乳，用于检测感染多种病毒的植物中的病毒。