新生児脳内の近赤外領域拡散スペクトルの研究

新生儿脑近红外扩散谱研究

• 批准号: 05557055
• 负责人: ONISHI Shoju
• 金额: $ 7.36万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557055/
• 关键词: continuous near infrared spectroscopy; diffusion spectra; multi-point method; cerebral Hb oxygen saturation; redox state of cytochrome c oxidase; neonate; premature infant; apne; HbO_2; cytochrome c oxidase; 脳酸素飽和; 多プロット化; 差スペクトル; cytochrome

In order to prevent and manage hypoxic-ischemic encephalopathy in neonates, it is very important to determine the oxygenated state of Hb, oxygen saturation (SbO_2), the redox state of cytochrome c oxidase, and cerebral blood volume in the cerebral tissue correctly and at real time. Using continuous near infrared spectroscopy (cNIRS) with multi-point method, we converted both the flattened and nonlinear diffusion spectra to both the non flattened and linear spectra and normalized different light pathlengths between respective spectra. Upon calculation of molar ratios of individual chromophore components existing in the light path of a given biological material, it was feasible to determine oxyhemoglobin, deoxyhemoglobin, SbO_2, and the redox state of cytochrome c oxidase in the cerebral tissue in vivo.Comparing the results measured on the scalp with the result measured directly on the dura of the piglets, a close agreement was proven to exist between the results of indirect and direct measurements. We studied the oxygenation of hemoglobin and the redox state of cytochrome c oxidase in full term and premature infants. SbO_2 was 60-70% in normal term infants and 50-75% in premature infants. The asphyxiated infant had a significantly higher total Hb and SbO_2 than the normal neonates controls. In apneic spell, the oxidized form of cytochrome c oxidase decreased after SbO_2.Thus, this study demonstrates that continuous bedside monitoring of cerebral hemoglobin oxygenation and the redox state of cytochrome c oxidase might become a useful index for evaluating cerebral hemodynamics and energy state.

准确、实时地测定新生儿脑组织Hb氧合状态、血氧饱和度（SbO_2）、细胞色素c氧化酶氧化还原状态和脑血容量，对预防和治疗新生儿缺氧缺血性脑病具有重要意义。利用多点连续近红外光谱（cNIRS）技术，将平面化和非线性扩散光谱转换为非平面化和线性光谱，并对光谱之间的不同光程进行归一化处理。通过计算给定生物材料光路中存在的单个发色团组分的摩尔比，可以测定活体脑组织中氧合血红蛋白、脱氧血红蛋白、SbO_2和细胞色素c氧化酶的氧化还原状态。将在头皮上测量的结果与直接在仔猪硬脑膜上测量的结果进行比较，证明间接测量和直接测量的结果非常一致。我们研究了足月和早产儿血红蛋白的氧合和细胞色素c氧化酶的氧化还原状态。正常足月儿SbO_2为60-70%，早产儿SbO_2为50-75%。窒息婴儿的总Hb和SbO_2明显高于正常新生儿。在无氧状态下，SbO_2后细胞色素c氧化酶的氧化形式减少。因此，本研究表明，连续床边监测脑血红蛋白氧合和细胞色素c氧化酶氧化还原状态可能成为评估脑血流动力学和能量状态的有用指标。

项目成果

Kenichi Isobe: "Clinical application of continuous near infrared spectroscopy-cerebral tissue oxygenation status of a severely asphyxiated infant-" Photomed Photobiol. 17. 67- (1995)

Kenichi Isobe：“连续近红外光谱的临床应用-严重窒息婴儿的脑组织氧合状态-”Photomed Photobiol。

Kenichi Isobe, Takashi Kusaka, Yoshiyasu Yaguchi, Kou Kawada, Mami Ishii, Tadashi Imai, Susumu Itoh, Shoju Onishi and Konomu Hirao: "Clinical application of continuous near infrared spectroscopy -cerebral tissue oxygenation status of a severely asphyxiate

Kenichi Isobe、Takashi Kusaka、Yoshiyasu Yaguchi、Kou Kawada、Mami Ishii、Tadashi Imai、Susumu Itoh、Shoju Onishi 和 Konomu Hirao：“连续近红外光谱的临床应用 - 严重窒息者的脑组织氧合状态

Takashi Kusaka, Kenichi Isobe, Kou Kawada, Yoshiro Ishii, Tadashi Imai, Susumu itoh, Shoju Onishi and Konomu Hirao: "Study of oxygenated state of hemoglobin and the redox state of cytochrome c oxidase in cerebral tissue under insulin-induced hypoglycemia

Takashi Kusaka、Kenichi Isobe、Kou Kawada、Yoshiro Ishii、Tadashi Imai、Susumu itoh、Shoju Onishi 和 Konomu Hirao：“胰岛素诱导低血糖下脑组织血红蛋白氧合状态和细胞色素 c 氧化酶氧化还原状态的研究

Kenichi Isobe, Tadashi Imai, Shoju Onishi, Ryo Fukuzaki, Mayumi Kondo, Masaki Hashimoto, Hitoshi Okada, Yoshiyasu Yaguchi, Susumu Itoh & Konomu Hirao: "Clinical application of continuous near infrared spectroscopy -Measurements of the oxygenated state of

矶部健一、今井正、大西翔十、福崎亮、近藤真由美、桥本正树、冈田均、矢口义康、伊藤进

今井 正: "新生児中枢神経障害の診断と管理" 産婦人科治療. 68. 22-23 (1994)

Tadashi Imai：“新生儿中枢神经系统疾病的诊断和治疗”妇产科治疗。 68. 22-23 (1994)。