权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Correlation between cerebral energy metabolism using ^<31>P-NMR and cerebral blood flow : Pathophisiological studies on hypoxic-ischemic encephalopathy in newborn piglets

^<31>P-NMR 脑能量代谢与脑血流量的相关性：新生仔猪缺氧缺血性脑病的病理生理学研究

基本信息

批准号：
05670974
负责人：
ONISHI Shoju
金额：
$ 1.34万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

For the pathophysiological study of hypoxic-ischemic encephalopathy, using the experimental condition of 15 min of graded hypoxia, we measured cerebral energy metabolism using phosphorus 31 nuclear magnetic resonanse spectroscopy (<@D131@>D1P-NMR) and cerebral blood flow (total CBF) with colored microspheres in seven newborn piglets within 24 hours after birth, which resemble human newborns in terms of cerebral growth. Total CBF before hypoxic insulte (control) was 50.2(]SY.+-。[)6.5 ml/min/100g. At 15 minutes after the start of hypoxic insulte (FiO<@D22@>D2=0.08), CBF increased to 90.1(]SY.+-。[)19.1ml/min/100g and became 1.8 times as much as the control. However, at 45 minutes after hypoxia (FiO<@D22@>D2=0.05), total CBF decreased to 42.2(]SY.+-。[)12.0 ml/min/100g and no significant difference was found compared to control. With regards to bradycardia (HR<100), total CBF was significantly decreased to 18.3(]SY.+-。[)9.4 ml/min/100g. On brain stem (pons, mid brain and medulla), blood flow was significantly increased at 45minutes, too, and was not significantly decreased on bradycardia. Compared to total CBF,considerably more blood flow was measured at 45 minutes and on bradycardia.There was a significant correlation between mean arterial blood pressure (MABP) and CBF (r=0.861, P<0.001). The autoregulation of total CBF was proved to be disturved. The Phosphocreatinine (PCr) /inorganic phosphate (Pi) and intracellular pH (pHi) did no change until total CBF reached levels below the levels control. The PCr/Pi and pHi suddenly decreased when total CBF decreased below the control. There was a significant correlation between total CBF and the PCr/Pi or pHi (r=0.843, p<0.001 and r=0.796, P<0.001). There was no significant correlation between PaO_2 and the PCr/Pi.Therefore, these results suggest that CBF is the most important factor in maintaining cerebral energy metabolism on hypoxic-ischemic encephalopathy.

为探讨缺氧缺血性脑病的病理生理学机制，在15 min分级缺氧的实验条件下，对7头出生后24 h内的新生仔猪（与人类新生儿脑发育相似），采用~（31）P核磁共振（<@D131@> D1 P-NMR）和彩色微球技术测定脑能量代谢和脑血流量。缺氧前（对照组）总CBF为50.2 ± 0.01（SY）。[)6.5 ml/min/100g。缺氧后15分钟（FiO<@D22@>D2=0.08），脑血流量增加至90.1 ± 0.01（SY ± 0.01）。[）19.1ml/min/100 g，是对照的1.8倍。然而，在缺氧后45分钟（FiO<@D22@>D2=0.05），总CBF下降至42.2（± SD）。[）12.0 ml/min/100 g，与对照组相比无显著性差异。对于心动过缓（HR<100），总CBF显著降低至18.3（± SD）。[)9.4 ml/min/100g。脑干（脑桥、中脑和延髓）的血流量在45分钟时也显著增加，心动过缓时无显著减少。平均动脉压（MABP）与CBF呈显著正相关（r=0.861，P<0.001）。总CBF的自动调节被证明受到干扰。磷酸肌酸酐（PCr）/无机磷酸盐（Pi）和细胞内pH值（pHi）没有变化，直到总CBF达到低于对照水平的水平。PCr/Pi和pHi在总CBF下降到对照组以下时突然下降。总CBF与PCr/Pi和pHi呈显著相关（r=0.843，P<0.001和r=0.796，P<0.001）。PaO_2与PCr/Pi无明显相关性，提示脑血流量是维持缺氧缺血性脑病脑能量代谢的重要因素。