AUTOMATED MULTI-CHANNEL LUMINOMETER FOR BIOLUMINESCENCE,DESIG AND ITS APPLICATION FOR CIRCADIAN RHYTHMICITY

采用16路外壳、光电倍增管、袖口计算机和Macintosh计算机制作了自动多路发光计，并编制了控制程序和数据分析程序。这种仪器可以跟踪两种不同生物的昼夜节律。生物发光鞭毛藻Gonyaulax polydra在生物发光、细胞分裂和光合活性等多种非本能生物事件中表现出昼夜节律性。在恒定光照条件下，它表现出节律性，周期约为23 h，但我们的光度计无法检测到肌酸的缩短周期作用。由于影响节律性的因素很多，我们无法在各种条件下（光照强度、监测时间、温度等）重现所报告的影响。基因修饰的蓝藻（引入细菌荧光素酶）显示出生物发光。光照强度低于鞭毛藻，但完全具有昼夜节律性，并具有温度补偿等重要特征。

Automated mutli-channel luminometer was made by using 16 channel housing, photomultiplier, pocket computer, and Macintosh computer and developted the programs for controlling the machine and analyzing the obtained data. This instrument allowed to follow circadian rhythmicity of two different organisms. Bioluminescent dinoflagellate Gonyaulax polyedra is well know to show circadian rhythmicities in several dinstinct biological events including bioluminescence, cell devision and photosynthetic activity. Under constant light conditions, it showed rhythmicity with a period of about 23 h, however, we could not detect the period-shortening effect of creatine by our luminometor. Since there are many factors influence on the rhythmicity, we could not reproduce the reported effects under various conditions (light intensity, monitoring time, temperature etc.) examined. Gene manipulated cyanobacteria (introducing bacteria luciferase) showed bioluminescence. The light intensity is lower than dinoflagellate but exactly showed circadian rhythmicity with several important characters such as temperature compensation.