Localized NMR spectroscopy and functional mapping of the brain.

局部核磁共振波谱和大脑功能图谱。

• 批准号: 05558098
• 负责人: YAMADA Kazuhiro
• 金额: $ 3.9万
• 依托单位: Oita Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05558098/
• 关键词: magnetic resonance spectroscopy; laser doppler flowmetry; regional lactate; regional blood flow; rabbit brain; hypoxia; oxygen delivery; 筋肉; 脳; 非侵襲的方法; 脳の局在機能; Nアセチルアスパラギン酸; 低酸素

Simultaneous applications of localized proton magnetic resonance spectroscopy (1H-MRS) and laser doppler flowmetry (LDF) allowed us to observe concurrent changes in regional lactate level and regional blood flow (rBF) in the anesthetized rabbit brain. The voxel (8mm-cubic) for 1H-MRS was located mostly in the thalamic area, and the rBF was recorded in the same area contralaterally. The localization in the 1H-MRS was assessed by using a phantom model and was proved to be practically satisfactory. During hypoxia, lactate increased gradually, while the rBF increased rapidly. Despite the increase in rBF,the oxygen delivery, which was the oxygen content in arterial blood (O2 CT) multiplied by the rBF,decreased. There was a linear correlation between the increase in lactate in last ten minutes during the thirty minutes hypoxia and deficiency in the oxygen delivery during hypoxia (correlation coefficience ; r2=0.68, n=14, P<0.001). In the subsequent recovery period, the rBF decreased slowly, while the O2 CT increased rapidly back to the control level. This rate of rFB recovery after hypoxia was linearly correlated with the increase in lactate (partial correlation coefficience ; r2=0.72, n=14, p<0.001).

同时应用局部质子磁共振波谱（1H-MRS）和激光多普勒血流仪（LDF），我们可以观察麻醉家兔脑区域乳酸水平和区域血流量（rBF）的同步变化。1H-MRS的体素（8mm立方）主要位于丘脑区域，rBF记录在同一区域的对侧。采用幻影模型对1H-MRS的定位进行了评估，结果令人满意。缺氧时乳酸逐渐升高，rBF迅速升高。尽管rBF增加，但动脉血中氧含量（O2 CT）乘以rBF的给氧量减少。缺氧30min时最后10min乳酸升高与缺氧时氧输送不足呈线性相关（相关系数r2=0.68, n=14， P<0.001）。在随后的恢复期，rBF下降缓慢，而O2 CT迅速回升至对照水平。缺氧后rFB恢复率与乳酸浓度的增加呈线性相关（偏相关系数；r2=0.72, n=14, p<0.001）。

项目成果

Yanai S,Nishimaru N,Soeda T,Yamada K: "Accumulation of intracellular lactate induces an increase in oxygen delivery during recovery from hypoxia." Fourth IBRO world congress of neuroscience.(Abstr.). 151. (1995)

Yanai S、Nishimaru N、Soeda T、Yamada K：“细胞内乳酸的积累会导致缺氧恢复过程中氧气输送量的增加。”

Yanai S,Nishimaru N,Soeda T,Yamada K: "Concurrent measurement of regional cerebral blood flow and metabolism using laser doppler flowmetry and volume selected NMR spectroscopy." Jpn J Physiol (Abstr.). 44. S260 (1994)

Yanai S、Nishimaru N、Soeda T、Yamada K：“使用激光多普勒血流计和体积选择核磁共振波谱同时测量局部脑血流量和代谢。”

Yanai,S.: "Accumulation of intracellular lactate induces on increase in oxygen delivery during recovery from hypoxia." Fourth IBRO World Congress of Neurosciences. Abstracts :. P151-B1.6 (1995)

Yanai,S.：“细胞内乳酸的积累会导致缺氧恢复过程中氧气输送量的增加。”

Yanai S,Nishimaru N,Soeda T,Yamada K: "Concurrent measurements of oxygen delivery and lactate production in rabbit thalamus." Neurosci Res (Abstr.). Suppl 19. S152 (1994)

Yanai S、Nishimaru N、Soeda T、Yamada K：“同时测量兔子丘脑的氧气输送和乳酸产生。”

S.Yanai,N.Nisimaru,T.Soeda,K.Yamada: "Concurrent measurments of regional cerebral blood flow and metabolism using laser duppler flowmetry and volume selected NMR spectroscopy during hypoxia." Japanese Journal of Physiology,Proceedings of the Annual. Suppl

S.Yanai、N.Nisimaru、T.Soeda、K.Yamada：“在缺氧期间使用激光双普勒流量计和体积选择核磁共振波谱同时测量局部脑血流量和代谢。”