Investigation of the prototype organisms of the chromophytes.

有色植物原型生物的研究。

• 批准号: 05044119
• 负责人: HORI Terumitsu
• 金额: $ 3.2万
• 依托单位: Institute of Biological Sciences, Univ.of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044119/
• 关键词: Algae; Chromophyte; Chrysophyta; Flagellar Apparatus; Phylogeny; Prototype; Sarcinochrysidales; 鞭毛装置; 摂食行動; 摂食装置; 鞭毛根系; 食胞; MTOC; 核分裂

It is generally believed in green plants that highly diverse ultrastructure of the cell including flagellar apparatus architecture is a reflection of their early evolutionary diversification. In this study we investigated many chrysophytes, including new marine species, ochromonadalean and sarcinochrysidalean algae on their cell structures and molecular basis to find out the prototype organisms of the chromophytes, since they have been believed to be an ancestral stock of heterokontes. Analysis of absolute configuration of the flagellar apparatus and other cell ultrastructure suggested that, 1) the order Sarcinochrysidales (sensu Gayral et Billard 1977) is probably an artificial taxon and consists of two monophyletic groups, one is directly linked to the pelagophycean algae and the other is closely related to the brown and yellowgreen algae, 2) a new species, Sulcochrysis biplastida, is a possible descendant of ancestral organisms that gave rise to the Pelagophyceae, Sarcinochrysidales sensu stricto, Pedinellophyceae and Dictyochophyceae, 3) another new species, Phaeomonas parva, is one of the most primitive heterokonts. The comparison of partial sequence data of the 18S rRNA suported it.

人们普遍认为，在绿色植物中，细胞的高度多样化的超微结构（包括鞭毛器结构）反映了它们早期进化的多样化。在这项研究中，我们研究了许多金藻，包括新的海洋物种、ochromonadalean 和 sarcinochrysidalean 藻类的细胞结构和分子基础，以找出有色植物的原型生物，因为它们被认为是异角藻的祖先。对鞭毛器和其他细胞超微结构的绝对构型的分析表明，1）Sarcinochrysidales目（sensu Gayral et Billard 1977）可能是一个人工分类单元，由两个单系群组成，一个与远洋藻类直接相关，另一个与褐藻和黄绿藻密切相关，2）一个新种，Sulcochrysis biplastida，可能是产生了 Pelagophyceae、Sarcinochrysidales sensu stricto、Pedinellophyceae 和 Dictyochophyceae 的祖先生物的后代，3) 另一个新物种，Phaeomonas parva，是最原始的异质藻之一。 18S rRNA部分序列数据的比较也支持了这一点。

项目成果

M.Kawachi and I.Inouye: "Ca^<2+>-mediated induction of the coiling of the haptonema in Chrysochromulina hirta(Prymnesiophyta=Haptophyta)." Phycologia. 33. 53-57 (1994)

M.Kawachi 和 I.Inouye：“Ca ^ 2 介导的 Chrysochromulina hirta（Prymnesiophyta = Haptophyta）中的 haptonema 卷曲诱导。”

S.Fujiwara,M.Sawada,N.Minaka,M.Kawachi and I.Inouye: "Molecular phylogenetic analysis of rbcL in the Prymnesiophyta." Journal of Phycology. 30. 863-871 (1994)

S.Fujiwara、M.Sawada、N.Minaka、M.Kawachi 和 I.Inouye：“Prymnesiophyta 中 rbcL 的分子系统发育分析”。

S.Fujiwara,M.Kawachi,I.Inouye and J.Someya: "The gene for ribosomal protein L27 is located on the plastid rather than the nuclear genome of the chlorophyll c-containing alga Pleuro-chrysis carterae." Plant Molecular Biology. 24. 253-257 (1994)

S.Fujiwara、M.Kawachi、I.Inouye 和 J.Someya：“核糖体蛋白 L27 的基因位于质体上，而不是含叶绿素 c 的藻类 Pleuro-chrysis carterae 的核基因组上。”

M.Kawachi and I.Inouye: "Functional roles of the haptonema and the spine scales in the feeding process of Chrysochromulina spinifera(Fournier)Pienaar et Norris (Haptophyta=Prymnesiophyta)." Phycologia. 34(印刷中). (1995)

M.Kawachi 和 I.Inouye：“Chrysochromulina spinifera(Fournier)Pienaar et Norris (Haptophyta=Primnesiophyta) 的摄食过程中的功能作用 (Haptophyta=Primnesiophyta) (1995)。”

T.Hori and J.C.Green: Oxford University Press. Mitosis and cell division. In "The Haptophyte Algae".(Eds.J.C.Green and B.S.C.Leadbeater), 91-109 (1994)

T.Hori 和 J.C.Green：牛津大学出版社。