The role of stress protein-reactive gammadeltaT cells in infection

应激蛋白反应性γδT细胞在感染中的作用

• 批准号: 05044178
• 负责人: MATSUZAKI Goro
• 金额: $ 3.2万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044178/
• 关键词: gammadeltaT cells; stress protein; bacterial infection; 自己反応性; hsp60

We have reported that gammadeltaT cells reactive to stress protein increase at early stage of Listeriainfection. We also found that stress protein-reactive gammadeltaT cells increase in syngeneic mixed lymphocyte reaction (MLR). From the observations, we estimated that self stress protein/bacterial stress protein-cross reactive gammadeltaT cells increase after bacterial infection. Therefore we analyzed self-reactive gammadeltaT cells to know further characteristics of stress protein-reactive gammadeltaT cells.We establisher 8 self-reactive gammadeltaT cell hybridomas. Four hybridomas expressed Vdelta5 and four hybridomas expressed Vdelta6. Response of seven out of eight self-reactive gammadeltaT cell hybridomas was blocked by anti stress protein (Hsp60) -monoclonal antibody ML30. Since response of gammadeltaT cell hybridomas with different T cell receptor was blocked with the ML30 antibody, we reasoned that Hsp60 molecule, but not ML30 epitope of Hsp60, participate in antigen recognition by the gammadeltaT cell hybridomas.

我们已经报道，在李斯特林感染的早期阶段，对应激蛋白有反应的GammadeltaT细胞增加。我们还发现，在同基因混合淋巴细胞反应(MLR)中，应激蛋白反应性伽马德兰T细胞增加。通过观察，我们估计在细菌感染后，自身应激蛋白/细菌应激蛋白-交叉反应性伽马德兰T细胞增加。因此，我们对自身反应性的GammadeltaT细胞进行了分析，以进一步了解应激蛋白反应性的GammadeltaT细胞的特征。4株杂交瘤表达Vdelta5，4株杂交瘤表达Vdelta6。抗应激蛋白(HSP60)-单抗ML30阻断了8个具有自身反应性的γ-T细胞杂交瘤的反应。由于具有不同T细胞受体的T细胞杂交瘤的反应被ML30抗体阻断，我们推测HSP60分子而不是HSP60的ML30表位参与了T细胞杂交瘤的抗原识别。

项目成果

Ivanyi,I.,Norton,P.,and Matsuzaki,G.: "Stress Proteins in Medicine" (in press), (1995)

Ivanyi,I.、Norton,P. 和 Matsuzaki,G.：“医学中的应激蛋白”（印刷中），（1995 年）

Ivanyi, J., Norton, P., and Matsuzaki, G.: (in press). Stress Proteins and Medicine, (1995)

Ivanyi, J.、Norton, P. 和 Matsuzaki, G.：（正在出版）。