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Studies on the biocompatibility of chitosan as biomaterials

壳聚糖生物材料的生物相容性研究

基本信息

批准号：
05045025
负责人：
NISHIMURA Shin-Ichiro
金额：
$ 3.84万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Synopsis. A novel and convenient method for the regioselective preparation of sulfated analogs of chitin and chitosan is described in relation with structure-biological activity study. Fully protected soluble derivatives were found to be useful intermediates for the syntheses of novel class of sulfated polysaccharides, (1*4)-2-acetamido-2-deoxy-3-O-sulfo-beta-D-glucopyranan and (1*4)-2-deoxy-2-sulfamido-3-O-sulfo-beta-D-glucopyranan. These compounds showed much higher inhibitory effect on AIDS virus infection in vitro than that by the known 6-O-sulfated derivative. This suggests that effective binding of chitin sulfates with gp 120 might be significantly affected by the sites of sulfation rather than the degree of substitution on sugar residues.An efficient and versatile solvent system for solubilization of chitin was found on the basis of calcium chloride-methanol systems and was applied to produce chitin fibers in the medical fields. These new fibers showed excellent properties as the coating materials of the artifitial organs owing to the biocompatibility and biodegradability.

概要。介绍了一种新的、简便的区域选择性制备甲壳素和壳聚糖硫酸酯类似物的方法，并对其结构和生物活性进行了研究。发现完全保护的可溶性衍生物是合成新型硫酸化多糖（1 × 4）-2-乙酰氨基-2-脱氧-3-O-磺基-β-D-吡喃葡萄糖和（1 × 4）-2-脱氧-2-磺酰胺基-3-O-磺基-β-D-吡喃葡萄糖的有用中间体。这些化合物在体外对艾滋病病毒感染的抑制作用比已知的6-O-硫酸酯衍生物高得多。这表明，甲壳素硫酸盐与糖蛋白120的有效结合可能受到硫酸化位点的影响，而不是糖残基上的取代度。在氯化钙-甲醇体系的基础上，发现了一种有效的和通用的溶解甲壳素的溶剂系统，并应用于制备医用甲壳素纤维。这些新型纤维具有良好的生物相容性和生物降解性，可作为人工器官的涂层材料。