Cytotoxicity of Superoxide and Nitric Oxide (NO)
超氧化物和一氧化氮 (NO) 的细胞毒性
基本信息
- 批准号:05045052
- 负责人:
- 金额:$ 3.2万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for international Scientific Research
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. MEASUREMENT OF SUPEROXIDE,NITRIC OXIDE (NO) and ONOO^-The generation of superoxide, NO and ONOO^- from human polymorphonuclear leukocytes were measured. For superoxide cytochrome c reduction and MCLA chemiluminescence methods were used. For NO,end uprodct of NO,nitrite+nitrate was measured by Griess method after reducing nitrite to nitrate. For ONOO^- we established a stable method by modifying the previously reported HPA-NO_2-HPLC method. Using these methods, the roles of these active molecules in various diseases were investigated and we found that the plausible cytotoxic molecules in polymorphonuclear leukocytes-mediated injury appears to be ONOO^- when the target of leukocytes is cardiac myocytes but superoxide is the major cytotoxic molecule when the target of leukocytes is endothelial cells.2. REAL-TIME MEASUREMENT OF SUPEROXIDE,NO and ONOO^-The real-time measurement of above mentioned molecules from isolated organs were also performed to investigate the cytotoxic mechanisms u … More nderlying the ischemia-reperfusion injury. Among several methods applied, we found that the oxymyoglobin method was applicable for that purpose.3. DEMONSTRATION OF ONOO^--MEDIATED INJURYTo demonstrate ONOO^--mediated tissue damage in various diseases such as liver cirrhosis, stomach ulcer and intestinal cancer, nitrotyrosine which is the reaction product of ONOO^- and tyrosine residues of protein was measured. Plasma and tissue concentration of nitrite+nitrate were also measured. The immunohistological staining using nitrotyrosine monoclonal antibodies was also performed for tissue samples obtained at the time of resection or through biopsy. The nitrotyrosine and nitrite+nitrate concentrations were significantly elevated in ulcer tissue. The increase correlated with severity ulcer. In contrast these levels remained low in the center of cancer and slightly elevated in the marginal portion of cancer. The nitrotyrosine staining method gave some positive staining in diseased portion but the specificity was shown to need further improvement. Less
1. 测定了人多形核白细胞中超氧化物、一氧化氮(NO)和ONOO^-的生成。超氧化物细胞色素c还原和MCLA化学发光方法。将亚硝酸盐还原为硝酸盐后,采用Griess法测定NO、NO末端根、亚硝酸盐+硝酸盐的含量。对已有报道的HPA-NO_2-HPLC法进行了改进,建立了稳定的ONOO^-测定方法。利用这些方法,我们研究了这些活性分子在各种疾病中的作用,我们发现在多形核白细胞介导的损伤中,当白细胞的目标是心肌细胞时,可能的细胞毒性分子是ONOO^-,而当白细胞的目标是内皮细胞时,超氧化物是主要的细胞毒性分子。超氧化物、一氧化氮和一氧化氮的实时测量-我们还对来自离体器官的上述分子进行了实时测量,以探讨其细胞毒性机制,从而进一步揭示缺血再灌注损伤的潜在机制。在应用的几种方法中,我们发现氧肌红蛋白法适用于该目的。证明ONOO^-介导的损伤为了证明ONOO^-在肝硬化、胃溃疡和肠癌等各种疾病中介导的组织损伤,测量了ONOO^-和酪氨酸残基蛋白的反应产物硝基酪氨酸。同时测定血浆和组织中亚硝酸盐+硝酸盐浓度。使用硝基酪氨酸单克隆抗体对切除或活检时获得的组织样本进行免疫组织学染色。溃疡组织中硝基酪氨酸和亚硝酸盐浓度显著升高。这种增加与溃疡的严重程度相关。相比之下,这些水平在癌症中心仍然很低,在癌症边缘部分略有升高。硝基酪氨酸染色法在病变部位有部分阳性染色,但特异性有待进一步提高。少
项目成果
期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakazawa H,Fukuyama H: "Is free radical generation an important component of ischemia/reperfusion injury? Part 1." Redox Report. 1. 177-179 (1995)
Nakazawa H、Fukuyama H:“自由基的产生是缺血/再灌注损伤的重要组成部分吗?第 1 部分。”
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- 影响因子:0
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Minhaz U,Tanaka M.Tukamoto H,Watanave K,Koide S,Shotsu A,Nakazawa H: ":Effect of MCI-186 on postishemic reperfusion injury in isolated rat heart." Free Rad Res. (in press).
Minhaz U、Tanaka M.Tukamoto H、Watanave K、Koide S、Shotsu A、Nakazawa H:“:MCI-186 对离体大鼠心脏缺血后再灌注损伤的影响。”
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- 影响因子:0
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- 通讯作者:
Ichimori K,Ishida H,Fukahori M,Nakzawa H,Murakami E: "Practical nitric oxide measurement employing a nitric oxide-selective electrode" Rev Sci Instrum. 65. 2714-2718 (1994)
Ichimori K、Ishida H、Fukahori M、Nakzawa H、Murakami E:“使用一氧化氮选择性电极进行实用一氧化氮测量”Rev Sci Instrum。
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- 影响因子:0
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Fukahori M,Ichimori K,Ishida H,Nakazawa H,Okino H: "Nitric oxide reversibly suppresses xanthine oxidase activity" Free Rad Res. 21. 203-212 (1994)
Fukahori M、Ichimori K、Ishida H、Nakazawa H、Okino H:“一氧化氮可逆地抑制黄嘌呤氧化酶活性”Free Rad Res。
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- 影响因子:0
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- 通讯作者:
Cadzheva V,Ichimori K,Nakazawa H,Raikov Z: "Superoxide scavenging activity of spin-labeled nitrosousa and triazene derivatives." Free Rad Res. 21. 177-186 (1994)
Cadzheva V、Ichimori K、Nakazawa H、Raikov Z:“自旋标记的亚硝基和三氮烯衍生物的超氧化物清除活性。”
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- 影响因子:0
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NAKAZAWA Hiroe其他文献
NAKAZAWA Hiroe的其他文献
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{{ truncateString('NAKAZAWA Hiroe', 18)}}的其他基金
Redox-regulation by active oxygen species / nitric oxide in cardiovascular system
心血管系统中活性氧/一氧化氮的氧化还原调节
- 批准号:
15390066 - 财政年份:2003
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Role of Inducible NO Synthase (iNOS) on Atherosclerosis -Study of iNOS-Knock out Mice-
诱导型一氧化氮合成酶 (iNOS) 对动脉粥样硬化的作用 -iNOS 的研究 - 敲除小鼠 -
- 批准号:
11838018 - 财政年份:1999
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of Electron Flow in Nitric Oxide Synthase
一氧化氮合酶中的电子流分析
- 批准号:
10045076 - 财政年份:1998
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Dynamics of nitric oxide (NO) in biological miliue and mechanism of NO-induced injury.
生物环境中一氧化氮(NO)的动态及NO诱导损伤的机制。
- 批准号:
09470174 - 财政年份:1997
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Investigation to develop a nitric oxide-selective electrode
研究开发一氧化氮选择性电极
- 批准号:
07557006 - 财政年份:1995
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Interaction and Cytotoxic Effect of Superoxide and NO in Reperfusion Injury
超氧化物和 NO 在再灌注损伤中的相互作用和细胞毒性作用
- 批准号:
05670635 - 财政年份:1993
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
The Clarification of Mechanisms for Electron Transfer and Identification of Radicals in Myocardium
心肌中电子传递机制的阐明和自由基的识别
- 批准号:
62570404 - 财政年份:1987
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Peroxynitrite Decomposition Catalyst and Nitric Oxide Donor for Endotoxemia
过氧亚硝酸盐分解催化剂和一氧化氮供体治疗内毒素血症
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8248638 - 财政年份:2012
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$ 3.2万 - 项目类别:
MUTATIONAL SPECTRA INDUCED BY NITRIC OXIDE, PEROXYNITRITE AND REACTIVE OXIDANTS
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6563792 - 财政年份:2002
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Direct and indirect effects of nitric oxide on nociceptive signal transduction at the trigeminal nucleus caudalis and mechanism for the regulation
一氧化氮对三叉神经尾核伤害性信号转导的直接和间接影响及其调节机制
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12470390 - 财政年份:2000
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Nitric oxide and butyrate affect HIF-1 activity and modulate function of tight junction in intestinal epithelial cell monolayers
一氧化氮和丁酸盐影响 HIF-1 活性并调节肠上皮细胞单层紧密连接的功能
- 批准号:
12671151 - 财政年份:2000
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$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
REACTIONS BETWEEN THIOLS, NITRIC OXIDE & PEROXYNITRITE
硫醇、一氧化氮之间的反应
- 批准号:
6307861 - 财政年份:2000
- 资助金额:
$ 3.2万 - 项目类别:
Superoxide and the nitric oxide-peroxynitrite pathway in renal ischaemia-reperfusion injury
肾缺血再灌注损伤中的超氧化物和一氧化氮-过亚硝酸盐途径
- 批准号:
nhmrc : 102554 - 财政年份:2000
- 资助金额:
$ 3.2万 - 项目类别:
NHMRC Project Grants
Interaction of nitric oxide and reactive oxygen on ADP-ribose-polymerase activation DNA damage
一氧化氮和活性氧相互作用对 ADP-核糖聚合酶激活 DNA 损伤的影响
- 批准号:
12671186 - 财政年份:2000
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$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MUTATIONAL SPECTRA INDUCED BY NITRIC OXIDE, PEROXYNITRITE AND REACTIVE OXIDANTS
一氧化氮、过氧亚硝酸盐和活性氧化剂诱导的突变谱
- 批准号:
6300203 - 财政年份:2000
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$ 3.2万 - 项目类别:
Nitric Oxide (NO) Production by Plant Nitrate Reductase
植物硝酸还原酶产生一氧化氮 (NO)
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12660048 - 财政年份:2000
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$ 3.2万 - 项目类别:
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REACTIONS BETWEEN THIOLS, NITRIC OXIDE & PEROXYNITRITE
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6118833 - 财政年份:1999
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