Investigation to develop a nitric oxide-selective electrode

研究开发一氧化氮选择性电极

• 批准号: 07557006
• 负责人: NAKAZAWA Hiroe
• 金额: $ 3.33万
• 依托单位: Tokai University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557006/
• 关键词: superoxide; nitric oxide; peroxynitrite; hemoglobin; NO-electrode; Cytotoxicity; Cylotoxicity

An NO-selective electrode was developed as an easily applicable tool for a real-time nitric oxide (NO) measurement. The working electrode (0.2 mm diam) was made from Pt/Ir alloy coated with a three-layred membrane. The counterelectrode was made from a carbon fiber. When a stable NO donor, S-nitroso-N-acetyl-dl-penicillamine, was applied, the electrode current increased in a dose-dependent fashion. The current and calculated NO concentration showed a linear relationship in the range from 0.2 nM (S/N=1) to 1 muM of NO.The response of the electrode was 1.14(]SY.+-。[)0.09s. The effects of temperature, pH,and chemicals other than NO on the electrode current were also evaluated. Electrodes which were placed in the luminal side of rat aortic rings exhibited 30 pA of current due to NO generation induced by the addition of 10<@D1-6@>D1M of acetylcholine. The current was eliminated in the presence of 50 muM N<@D1G@>D1-monomethyl-L-arginine, an inhibitor of NO synthase. Thus, this NO-selective electrode is applicable to real-time NO assay in biological systems.NO+2H_2O*NO^-_+4H^++3e^-.A positive voltage of approximately 0.8 V was applied t discriminate against the current from possible contaminant

NO选择电极是一种易于应用的实时测量一氧化氮（NO）的工具。工作电极（直径0.2 mm）由镀有三层膜的Pt/Ir合金制成。对电极由碳纤维制成。当使用稳定的NO供体s -亚硝基-n -乙酰-dl-青霉胺时，电极电流呈剂量依赖性增加。电流与计算得到的NO浓度在0.2 nM (S/N=1) ~ 1 muM范围内呈线性关系。电极的响应为1.14(]SY.+- . [)0.09s。温度、pH值和除NO以外的化学物质对电极电流的影响也进行了评估。电极放置在大鼠主动脉环管腔侧，由于加入10<@D1-6@>D1M的乙酰胆碱诱导NO产生，电流为30 pA。电流在50 muM N<@D1G@> d1 -单甲基- l-精氨酸（NO合成酶抑制剂）存在下消除。因此，该NO选择电极适用于生物系统中实时NO测定。NO+2H_2O*NO^-_+4H^++3e^-。施加约0.8 V的正电压以区分来自可能污染物的电流

项目成果

Ishiyama S,et al: "Nitric Oxide contributes to the progression of myocardial damage in experimental autoimmune myocarditis in rats." Circulation. (in press).

Ishiyama S 等人：“一氧化氮会导致大鼠实验性自身免疫性心肌炎心肌损伤的进展。”

Minhaz U,Fukahori M,Nakazawa H: "Early reperfusion induces alveolar injury in pulmonary embolism." Chest. (in press).

Minhaz U、Fukahori M、Nakazawa H：“早期再灌注会导致肺栓塞中的肺泡损伤。”

Nakazawa H,Genka C,Fujishima M: "Pathological aspects of active oxygens/free radicals" Jpn J Physiol. 46 (1). 15-32 (1996)

Nakazawa H，Genka C，Fujishima M：“活性氧/自由基的病理学方面”Jpn J Physiol。

福山 直人,中澤 博江: "NOの多面的な作用機序" 医学のあゆみ. 9. 529-534 (1995)

Naoto Fukuyama、Hiroe Nakazawa：“NO 作用的多方面机制”《医学史》9. 529-534 (1995)。

福山直人、石田英之、ら: "Peroxynitrite(ONOO-)の心筋細胞障害性" 心筋の構造と代謝. 18. 331-334 (1995)

Naoto Fukuyama、Hideyuki Ishida 等人：“过氧亚硝酸盐 (ONOO-) 的心肌细胞毒性”《心肌结构和代谢》18. 331-334 (1995)。