Investigation to develop a nitric oxide-selective electrode

一氧化氮（NO）选择性电极的开发作为一个易于应用的工具，用于实时测量。工作电极（0.2mm直径）由涂覆有三层膜的Pt/Ir合金制成。反电极由碳纤维制成。当一个稳定的NO供体，S-亚硝基-N-乙酰基-dl-青霉胺，施加，电极电流增加，在一个剂量依赖性的方式。NO浓度在0.2 nM（S/N=1）~ 1 μ M范围内与电流呈线性关系，电极响应值为1.14（μ S ± N）。[）0.09s。温度，pH值，和化学品以外的NO对电极电流的影响也进行了评价。将电极置于大鼠主动脉环的腔侧，由于加入10<@D1-6@> D 1 M的乙酰胆碱诱导产生NO，因此显示出30 pA的电流。该电流在50 μ M NO合酶抑制剂N<@D1G@>D1-单甲基-L-精氨酸存在下被消除。NO+2H_2O*NO^-+4 H ^++3e ^-.在0.8V左右的正电压下，可将电流与可能的污染物区分开来

An NO-selective electrode was developed as an easily applicable tool for a real-time nitric oxide (NO) measurement. The working electrode (0.2 mm diam) was made from Pt/Ir alloy coated with a three-layred membrane. The counterelectrode was made from a carbon fiber. When a stable NO donor, S-nitroso-N-acetyl-dl-penicillamine, was applied, the electrode current increased in a dose-dependent fashion. The current and calculated NO concentration showed a linear relationship in the range from 0.2 nM (S/N=1) to 1 muM of NO.The response of the electrode was 1.14(]SY.+-。[)0.09s. The effects of temperature, pH,and chemicals other than NO on the electrode current were also evaluated. Electrodes which were placed in the luminal side of rat aortic rings exhibited 30 pA of current due to NO generation induced by the addition of 10<@D1-6@>D1M of acetylcholine. The current was eliminated in the presence of 50 muM N<@D1G@>D1-monomethyl-L-arginine, an inhibitor of NO synthase. Thus, this NO-selective electrode is applicable to real-time NO assay in biological systems.NO+2H_2O*NO^-_+4H^++3e^-.A positive voltage of approximately 0.8 V was applied t discriminate against the current from possible contaminant