Parallel bioreactor system

平行生物反应器系统

• 批准号: 525666046
• 金额: --
• 依托单位: Universität des Saarlandes
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525666046?language=en
• 关键词: Parallel; bioreactor; system

The applying consortium deal with the development of novel processes for the discovery, synthesis, structure elucidation and production of bioactive natural products of microbial origin. In recent years, more than 100 new bioactive natural substances have been discovered within the consortium, incuding several novel scaffolds. However, the newly discovered natural substances can often only be produced in small quantities. The isolated small amounts are usually only sufficient for carrying out the structural elucidation of a new natural substance, but not for detailed bioactivity studies as an essential step in evaluating the potential and possible further pharmaceutical development. Quantities in the higher milligram range are required for the componds development. So far, these quantities have been produced by massive parallelization in non-optimized shake flask cultures. This requires up to 200 parallel batches with a flask volume of 100 – 1000 mL. An increase in the isolated amounts of material by further increasing the working volumes is not possible with the existing infrastructure. Therefore, an optimized production of the substances in controlled bioreactors is essential for the efficient implementation and further development of research. The working groups have two individual fermenters (4 and 8 liters), which are in principle suitable for production, but can only be used suboptimal due to the lack of an infrastructure for optimizing the production conditions. A direct transfer of the production conditions from a shake flask to such a bioreactor is not possible. A system with several small fermenters is therefore required for optimization and process development, which enables the systematic adjustment of cultivation parameters in an experiment. Such a facility is not available in the working groups and, due to the large number of the experiments to be carried out, is also not accessible through cooperation. In order to close this equipment gap, the purchase of a system with 8 parallel bioreactors is requested. The number of individual reactors results from the need to be able to investigate the influence of several cultivation conditions on production in one experiment and to have sufficient biological replicates available. The fermentation system applied for should have standard and essential equipment parts such as temperature, pH and level controls, measurement of dissolved oxygen and redox potential as well as fermentation gases, gassing with mass flow meter and pump modules for feeding of various compounds, which impact the process (lye, acid, antifoam agent) and nutrients.

申请财团处理新工艺的开发，用于发现，合成，结构鉴定和微生物来源的生物活性天然产物的生产。近年来，在该研究组中发现了100多种新的生物活性天然物质，其中包括几种新型支架。然而，新发现的天然物质往往只能少量生产。分离的少量通常仅足以进行新天然物质的结构解析，而不足以进行详细的生物活性研究，作为评价潜在和可能的进一步药物开发的必要步骤。在更高的毫克范围内的杂质是成分开发所必需的。到目前为止，这些数量是通过非优化摇瓶培养中的大规模平行化产生的。这需要多达200个平行批次，容量瓶体积为100 - 1000 mL。在现有的基础设施下，通过进一步增加工作体积来增加隔离的材料量是不可能的。因此，在受控生物反应器中优化物质的生产对于有效实施和进一步发展研究至关重要。工作组有两个单独的发酵罐（4升和8升），原则上适用于生产，但由于缺乏优化生产条件的基础设施，只能使用次优。不可能将生产条件从摇瓶直接转移到这种生物反应器。因此，需要具有几个小发酵罐的系统进行优化和工艺开发，这使得能够在实验中系统地调整培养参数。工作组没有这种设施，而且由于要进行大量实验，也无法通过合作获得这种设施。为了弥补这一设备缺口，需要购买一个有8个平行生物反应器的系统。单个反应器的数量是由于需要能够在一个实验中研究几种培养条件对生产的影响，并具有足够的生物重复。所申请的发酵系统应具有标准和必要的设备部件，如温度、pH值和液位控制，溶解氧和氧化还原电位以及发酵气体的测量，使用质量流量计和泵模块进行充气，用于进料各种化合物，这些化合物会影响工艺（磷酸盐、酸、消泡剂）和营养素。