Parallel bioreactor system

在可重复和可控制的条件下平行培养微生物是描述微生物特性的重要组成部分。在我的工作组中，我们处理生产生物基材料和化学品的工艺和菌株的优化问题。重点主要放在生物基和可生物降解的多糖上，我们使用活体工程来实现。通过这种方法，我们创造了许多不同的菌株变种，我们检查它们在可重现和可控制的条件下的产物形成。由于新的胞外多糖变异体的化学结构的改变，在生产率上也有很大的差异。由于多糖的生物合成高度依赖于能量，从而依赖于氧气，因此这些变体中的许多只能在生物反应器中的最佳条件下生产。在下文中，我们描述了这些新的多糖变异体的基本结构-功能关系，从而衍生出新功能的方法。在进一步的步骤中，对生物合成途径进行特定菌株的优化，以提高生产率。使用的菌株组合包括革兰氏阴性和革兰氏阳性细菌，但也包括丝状真菌和微藻。对于微藻来说，在可重复的条件下在光生物反应器中进行培养是必不可少的，可以使用不同波长的靶向照明，以分析光强度和波长对生长和产物形成的影响。除了对微生物多糖的研究外，我们最近还在开发利用C1化合物的菌株，这种化合物可以从合成气体中生产出来。为此，我们使用了例如甲烷芽孢杆菌，它以前被认为是非常难以遗传的。然而，基于我们开发的分子生物学工具(CRISPR-CAS)，高效的处理现在似乎是成功可行的。培养甲烷假单胞菌需要50°C的稳定温度，而培养容器不会蒸发，这极大地干扰了产品和物流的核算。所要求的并行生物反应器系统满足了所有这些要求，并提供了一个气体混合站、废气分析、稳定的温度控制和使用光棒的可能性，可以服务于我们工作组所有必要的并行培养实验。

The parallel cultivation of microorganisms under reproducible and controllable conditions is an essential component for the characterization of microorganisms. In my working group we deal with the optimization of processes and strains for the production of biobased materials and chemicals. The focus is mainly on bio-based and biodegradable polysaccharides, for which we use in vivo engineering. By this approach, we create many different strain variants, which we examine for their product formation under reproducible and controllable conditions. Due to the modified chemical structures of the new exopolysaccharide variants, there are also strong differences in productivity. Due to the highly energy- and thus oxygen-dependent biosynthesis of the polysaccharides, many of these variants can only be produced under optimal conditions in bioreactors. In the following, we characterize the basic structure-functional relationships of these new polysaccharide variants, and thus derive approaches for new functionalities. In further steps, the strain-specific optimization of the biosynthesis pathways is carried out in order to increase productivity. The portfolio of strains used includes Gram-negative and Gram-positive bacteria, but also filamentous fungi and microalgae. For microalgae, cultivation in photobioreactors under reproducible conditions is essential, with the possibility of targeted illumination with different wavelengths, in order to analyze the influence of light intensity and wavelength on growth and product formation. In addition to research on microbial polysaccharides, we are recently working on the development of strains which utilize C1 compounds, which can be produced from e.g. synthesis gas. For this purpose, we use e.g. Bacillus methanolicus, which was previously considered to be very difficult to genetic accessibility. Based on molecular biology tools (CRISPR-Cas) developed by us, however, efficient processing now seems to be successfully feasible. For the cultivation of B. methanolicus stable temperatures of 50 °C are necessary, without evaporation from the cultivation vessels, which massively disturbs the accounting of the products and material flows.The requested parallel bioreactor system meets all these requirements and offers with a gas mixing station, exhaust gas analysis, stable temperature control and the possibility of using light rods a device that can serve all necessary parallel cultivation experiments in our working group.