Studies on Safety and Effectiveness of Dentin Pretreatment Agents, Pulp Capping Agents and Base Materials

牙本质预处理剂、盖髓剂及基材的安全性和有效性研究

• 批准号: 05304043
• 负责人: SHIMOKOBE Hirokata
• 金额: $ 14.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304043/
• 关键词: Dentin Pretreatment Material; Antibacterial Capping Agent; Base Cement; Dentin Primer; Phytic Acid; EDTA LaNa; Tannic Acid; Cytotoxity; 抗菌性覆髄剤; ベース材

1.A new cytotoxic test of dental materials on human neutrophils was established by determining the production of O_2^- and the release of LDH.The addition of collagen-combined BMP induces differentiation of bone marrow stromal cells into osteoblasts.(shimokobe, Matsui and Inoue)2.The antibacterial capping agent(alpha-TCP with mixed antibacterial drugs)effectively disinfected bacterially contaminated pulp tissue without destroying surrounding pulp tissue(Iwaku).3.The dentin priming by the aqueous solution of the ethylene glycol or 1,6-hexanediol was considered to be desirable because the contraction gap formation of the light activated resin compostite was prevented. And the possible side effect on the skin tissue by the methacrylate derivatives was avoided by using the diol solutions.(Hisamitsu)4.When the dentin surface was treated with chelating agent(phytic acid)and metallic ion(Sn^<2+>), shear bond strength of resin to dentin did not change after 1,000 thermo-cycled(Kohno and Sakamo … More to).5.Glass ionomer lining materials showed the strongest cellular growth of ten pulp capping materials tested which did not show any mutagenic potential using UMU-test kit(Aono).6.The dentin surface treated with La-containing conditioner showed higher hardness, better acid resistance and higher bond strength with resin bonding agent(Hara and Senda).7.Among three experimental penetrometers of Al, Cu or Ti for objective measuring of radiopacity of base cements, Ti-penetrometer revealed the best results(Fujii and Narikawa).8.Increased concentration of tannic acid and increased incubation times in the acid effectively decreased the dissolved amount of dentin collagen(Shintani).9.Not only calcium hydroxide but also alkyl salicylates influenced the antimicrobial activity of the set calcium hydroxide cements(Nagasawa).10.The grinding of tetracalcium phosphate(TTCP)increased the mcchanical strength of the cement based on polymethyl vinyl ether-maleic acid and TTCP,decreased its solubility, and accelerated the hydroxyapatite formation(Matsuya). Less

1.建立了一种新的牙科材料对中性粒细胞的细胞毒性试验方法，通过测定中性粒细胞产生O_2^-和释放乳酸脱氢酶来检测牙科材料对中性粒细胞的细胞毒性。添加胶原结合的BMP可以诱导骨髓基质细胞向成骨细胞分化。2.抗菌盖盖剂(α-磷酸三钙和混合抗菌药物)有效地消毒了被细菌污染的牙髓组织，而不破坏周围的牙髓组织(Iwaku)。3.用乙二醇水溶液或1，6-己二醇水溶液进行牙本质预充是理想的，因为防止了光活化树脂组分收缩间隙的形成。当牙本质表面用螯合剂(植酸)和金属离子(Sn2+)处理时，树脂与牙本质的剪切粘结强度在1000次热循环后没有变化(Kohno和Sakamo…5.玻璃离子衬里材料在所测试的10种盖髓材料中细胞生长最强，UMU-test Kit(Aono)未显示出任何诱变潜力。6.经含La修饰剂处理的牙本质表面显示出更高的硬度、更好的耐酸性以及与树脂粘结剂(Hara和Senda)更高的粘结强度。7.在三种用于客观测量基牙本质放射不透明度的实验渗透仪中，钛渗透仪显示效果最好的是Fujii和Narikawa。8.增加单宁酸的浓度和增加在酸中孵育的时间有效地减少牙本质胶原(Shintani)的溶解量。9.不仅氢氧化钙而且烷基水杨酸盐也影响固定的氢氧化钙粘固剂(长泽)的抗菌活性。10.磷酸四钙(TTCP)的研磨增加了聚甲基乙烯基醚-马来酸和TTCP基水泥的机械强度，降低了其溶解度，促进了羟基磷灰石(Matsuya)的形成。较少

项目成果

Kunihiro Yoshiba: "Effects of Antibacterial Capping Agents on Dental Pulps of Monkeys Mechanically Exposed to Oral Microflora." Journal of Endodontics. 21(in press). (1995)

Kunihiro Yoshiba：“抗菌加盖剂对机械暴露于口腔微生物群的猴子牙髓的影响。”

Chihiro Tani: "Effect of Filler Content on Bonding Efficacy of 4-META MMA/TBB Bonding Agent." Dental Materials Journal. 13. 131-137 (1994)

Chihiro Tani：“填料含量对 4-META MMA/TBB 粘结剂粘结功效的影响。”

薄井秀樹: "キレート化剤と金属イオンを組み合わせたプライマーの各種ボンディング剤への応用" 日本歯科保存学雑誌. 36. 1031-1053 (1993)

Hideki Usui：“结合螯合剂和金属离子的底漆在各种粘合剂中的应用”日本保守牙科杂志 36. 1031-1053 (1993)。

Kanamori K.: "Effect of Dentin Pretreatment with EDTA-La-Na on Resin-Dentin Bonding" Aichi-Gakuin Dental Science. 6. 17-23 (1993)

Kanamori K.：“EDTA-La-Na 牙本质预处理对树脂-牙本质粘合的影响”爱知学院牙科科学。

Yoko Matsuya: "Effect of abietic acid and poly(methyl methacrylate) on the dissolution process of zinc oxide-eugenol cement" Biomaterials. 15. 307-314 (1994)

Yoko Matsuya：“松香酸和聚甲基丙烯酸甲酯对氧化锌-丁子香酚水泥溶解过程的影响”生物材料。