Study on the way of protection and prevention of HFRS in laboratory animal experiments.

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We found that nude rat was very sensitive in the hantavirus infection. In order to study the mechanism of hantavirus infection, nude rats were used as an indicator of the detection of infection.First of all, we have produced rats having different antibody titers by injecting different number of active viruses. In general, rats having lower antibody titers showed lower infectivity to indicator nude rats in comparison with the one having higher titers. However, almost all of the rats experimentally infected with hantavirus B-1 strain showed infectivity to nude rats except the rats having the antibody titer less than x128 for all the time observed. To check the virus genome RNA,RT-PCR was performed. The detection limit was 0.1 pfu in our experimental condition. Genome RNA was detected in the early phase of viral infection and also in reactivation phase. However, it was not detected at the later stage of infection even if the antibody titer was high. In this experimentally infected rats, all showed positive in western blotting were infective to nude rats.