Study on the way of protection and prevention of HFRS in laboratory animal experiments.

实验动物实验中HFRS的防护和预防途径研究

• 批准号: 05306003
• 负责人: NISHIMUNE Yoshitake
• 金额: --
• 依托单位: Osaka University Research Institute for Microbial Diseases,
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05306003/
• 关键词: HFRS,; hantavirus infection,; zoonoses; nude rat,; 腎症候性出血熱(HFRS); 低抗体価rat; 検査法の確立; 抵抗体価rat

We found that nude rat was very sensitive in the hantavirus infection. In order to study the mechanism of hantavirus infection, nude rats were used as an indicator of the detection of infection.First of all, we have produced rats having different antibody titers by injecting different number of active viruses. In general, rats having lower antibody titers showed lower infectivity to indicator nude rats in comparison with the one having higher titers. However, almost all of the rats experimentally infected with hantavirus B-1 strain showed infectivity to nude rats except the rats having the antibody titer less than x128 for all the time observed. To check the virus genome RNA,RT-PCR was performed. The detection limit was 0.1 pfu in our experimental condition. Genome RNA was detected in the early phase of viral infection and also in reactivation phase. However, it was not detected at the later stage of infection even if the antibody titer was high. In this experimentally infected rats, all showed positive in western blotting were infective to nude rats.

结果表明，裸鼠对汉坦病毒感染非常敏感。为了研究汉坦病毒感染的机制，我们使用裸大鼠作为检测感染的指标。首先，我们通过注射不同数量的活性病毒来产生具有不同抗体滴度的大鼠。一般而言，与具有较高滴度的大鼠相比，具有较低抗体滴度的大鼠对指示裸大鼠显示出较低的感染性。然而，几乎所有用汉坦病毒B-1株实验感染的大鼠都显示出对裸大鼠的感染性，除了在所有观察时间内抗体滴度低于× 128的大鼠。为了检查病毒基因组RNA，进行RT-PCR。在我们的实验条件下，检测限为0.1 pfu。在病毒感染的早期和再激活期都能检测到基因组RNA。但在感染后期，即使抗体滴度高，也未检出。在本实验感染的大鼠中，所有免疫印迹阳性的大鼠均能感染裸鼠。

项目成果

Dhomae K.,Nishimune Y: "Protection against Hantavirus infection by dams immunity transferred vertically to neonates." Archives Virology. (in press).

Dhomae K.，Nishimune Y：“通过垂直转移给新生儿的母体免疫力来防止汉坦病毒感染。”

Yoshimatsu,K.,Arikawa,J.and Kariwa,H.: "Application of recombinant baculovirus expressed hantavirus nucleoc apsid protein as a di agnostic antigen in IFA test:cross reactive among the three different serotypes of hemorrhagic fever with renal syndrome(HFRS

Yoshimatsu,K.、Arikawa,J.和Kariwa,H.：“重组杆状病毒表达的汉坦病毒核壳蛋白作为IFA试验中的诊断抗原的应用：肾综合征出血热（HFRS）三种不同血清型之间的交叉反应

有川 二郎: "わが国のゲッ歯るいにおけるハンタウイルス感染の疫学的研究と血清診断" 臨床とウイルス. 23. :11-18 (1995)

Jiro Arikawa：“日本大麦汉坦病毒感染的流行病学研究和血清诊断”《临床和病毒学》23。：11-18（1995）。

Dohmae K.,Koshimizu U.,Nishimune Y.: "In udtero and mammary transfer of Hantavirus antibody from dams to infant rats." Laboratory Animal Science. (in Press).

Dohmae K.、Koshimizu U.、Nishimune Y.：“在子宫和乳房中，汉坦病毒抗体从母鼠转移到幼鼠。”

有川 二郎: "わが国の野性げっ歯類におけるハンタウイルス感染の疫学的研究と血清診断" 臨床とウイルス. 24. (1994)

有川次郎：“日本野生啮齿动物汉坦病毒感染的流行病学研究和血清诊断”《临床和病毒学》24。（1994）