Animal Model of Male Sterility ; Differentiation of Germ Cell in Sl, W Mutant Mice.

雄性不育动物模型；

• 批准号: 01480511
• 负责人: NISHIMUNE Yoshitake
• 金额: $ 2.94万
• 依托单位: Research Institute for Microbiological Diseases, Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480511/
• 关键词: Differentiation; Cryptorchidism; mutant mouse; animal model; spermatogenesis; germ cell; W gene; Sl gene

The effects of Steel-Dickie (Sl^d) mutation and W mutations on testicular germ cell differentiation were investigated using experimental cryptorchidism and its surgical reversal. In Sl^d/+ cryptorchid testes the maintenance of undifferentiated type-A spermatogonia was impaired and their numbers decreased. In contrast, the proliferative activity of type-A spermatogonia in the cryptorchid testis of mutant mice appeared normal as judged by their progression through the cell cycle. Surgical reversal of cryptorchidism resulted in regenerative differentiation of type-A spermatogonia which remained in Sl^d/+ cryptorchid testes was strongly impaired, particularly at two steps of cellular differentiation, from type-A spermatogonia to intermediate or type-B spermatogonia and at meiotic division.The effect of W, W^f, W^<sh>, and W^v mutations on spermatogenesis in mice was also examined by using experimental cryptorchidism and its surgical reversal. The results indicate that the W gene also exerts its effect on spermatogenic cell differentiation at the same steps as SI gene does. These results of regenerative differentiation of testicular germ cells in both mutant mice indicate that signal transduction between Sl (ligand) and W (c-kit receptor) gene products is important in spermatogenesis of adult mouse.

利用实验性隐睾及其手术逆转研究Steel-Dickie（Sl^d）突变和W突变对睾丸生殖细胞分化的影响。在Sl^d/+隐睾中，未分化的A型精原细胞的维持受损，其数量减少。与此相反，A型精原细胞在突变小鼠隐睾睾丸的增殖活性出现正常的判断，通过细胞周期的进展。隐睾手术逆转导致Sl^d/+隐睾中A型精原细胞的再生分化受到严重损害，特别是在A型精原细胞向中间型或B型精原细胞分化和减数分裂两个细胞分化阶段<sh>。结果表明，W基因在生精细胞分化中的作用与SI基因相同。这两种突变小鼠睾丸生殖细胞再生分化的结果表明，S1（配体）和W（c-kit受体）基因产物之间的信号转导在成年小鼠精子发生中是重要的。

项目成果

H. Kuroda et al.: "Differentiation of germ cells in seminiferous tubules transplanted to testes of germ cell-deficient mice of W/W^v and Sl/Sl^d genotypes." J. Cell Physiol. 139. 329-344 (1989)

H. Kuroda 等人：“移植到 W/W^v 和 Sl/Sl^d 基因型生殖细胞缺陷小鼠睾丸中的生精小管中生殖细胞的分化。”

H.Kuroda et al.: "Differention of germ cells in sイminiferous tubules transplanted to testes of germ cellーdeficient mice of W/(W^v) and (Sl)/(Sl^d) genofype" J. Cell. Physiol.139. 329-344 (1989)

H. Kuroda 等人：“移植到 W/(W^v) 和 (Sl)/(Sl^d) 基因型生殖细胞缺陷小鼠的测试中的生殖细胞的差异”J. Cell。 139. 329-344 (1989)

Y.Nishimune et al.: "Secretion of plasminogen activator in response to Follicle-Stimulating Hormone in culture medium of human testicular cells from biopsy specimens." J.of Andrology. 10. 283-288 (1989)

Y.Nishimune 等人：“活检标本中人睾丸细胞培养基中纤溶酶原激活剂响应卵泡刺激素的分泌。”

Y. Tajima et al.: "SteelーDickie(Sl^d)mutation affects both maintenance and differentiation of testicular germ cells" J. Reprod. Fertil.

Y. Tajima 等人：“Steel-Dickie(Sl^d) 突变影响睾丸生殖细胞的维持和分化”J. Reprod。

M.Maekawa et al.: "Plasminogen activator secreted by Sertoli cells in response to Retinoids" Mol.Androl.1. 331-336 (1989)

M.Maekawa 等人：“支持细胞响应类维生素A而分泌的纤溶酶原激活剂”Mol.Androl.1。